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In-vitro rapid propagation method for gordon euryale seeds

A Gorgon fruit, in vitro technology, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of increasing the workload and difficulty of breeders, unstable characters of the offspring of Gorgon, and achieves improved breeding efficiency and shortened time. The effect of breeding years

Active Publication Date: 2021-11-02
江苏绿冶苗木科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the breeding of new varieties of Gorgon fully relies on natural mutagenesis and hybrid breeding. The traits of the offspring of Gorgon obtained by the above methods are extremely unstable, which greatly increases the workload and difficulty of breeders. However, the in vitro regeneration optimization system of Gorgon can be widely used in breeding Rapid propagation and genetic transformation of seedlings, and provide a fast and effective method for the preservation of Gorgon seed resources, break the dormancy of Gorgon seeds, break the constraints of seasons on Gorgon R&D and production, and meet market needs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] For explant treatment, take 100 Gorgon seed peeled embryo seeds (full, complete and disease-free with a diameter of more than 1.0cm) and wash them for germination induction culture. Soak the seeds in pure water and store them sealed in a refrigerator at 4°C. Wash them for 3 to 5 days during the period Once and replace the pure water, take it out after 1 month, remove the shell with a special sheller for Gorgon fruit, peel off the seed shell of the selected seeds, and keep the complete seed embryo, endosperm and transparent protective film on the surface, and the peeled seed shell is complete The seeds were rinsed with tap water and soaked in 800 times 50% carbendazim solution for 20 minutes, then rinsed with running water for 1-2 hours, then transferred to sterile tissue culture bottles, and moved to the ultra-clean workbench. The disinfection sequence was 75% Disinfect with alcohol for 45 seconds, rinse with sterile water for 3 to 5 times, disinfect with 0.1% HgCl2 solu...

Embodiment 2

[0040] For explant treatment, take 100 Gorgon seed peeled embryo seeds (full, intact and disease-free with a diameter of more than 1.0cm) and wash them for germination induction culture. Soak the seeds in pure water and store them in a refrigerator at 0°C. Wash them for 3 to 5 days during the period. Once and replace the pure water, take it out after 1 month, rinse with tap water, soak in 800 times 50% carbendazim solution for 20min, then rinse with running water for 1h-2h, then transfer to a sterile tissue culture bottle, and move to ultra-clean For the workbench, the sequence of disinfection is 75% alcohol disinfection for 45 seconds, rinsing with sterile water for 3 to 5 times, disinfection with 0.1% HgCl2 solution for 12 minutes, and cleaning with sterile water for 6 to 8 times. Blot the surface moisture with bacterial filter paper, and dry the surface moisture with sterile air.

[0041] Seed dormancy breaking and germination treatment, shelled with a special sheller for G...

Embodiment 3

[0045] For explant treatment, take 100 Gorgon seed peeled embryo seeds (full, intact and disease-free with a diameter of more than 1.0cm) and wash them for germination induction culture. Soak the seeds in pure water and store them in a refrigerator at 5°C, and wash them for 3 to 5 days Once and replace the pure water, take it out after 1 month, remove the shell with a special sheller for Gorgon fruit, peel off the seed shell of the selected seeds, and keep the complete seed embryo, endosperm and transparent protective film on the surface, and the peeled seed shell is complete The seeds were rinsed with tap water and soaked in 800 times 50% carbendazim solution for 20 minutes, then rinsed with running water for 1-2 hours, then transferred to sterile tissue culture bottles, and moved to the ultra-clean workbench. The disinfection sequence was 75% Disinfect with alcohol for 45 seconds, rinse with sterile water for 3 to 5 times, disinfect with 0.1% HgCl2 solution for 12 minutes, an...

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Abstract

The invention relates to an in-vitro rapid propagation method for gordon euryale. The in-vitro rapid propagation method for the euryale ferox comprises the following steps of firstly, selecting seeds, namely selecting euryale ferox seeds newly harvested in the current year; carrying out seed shell treatment, carrying out explant treatment, establishing a gordon euryale seed sterile rapid propagation system, and carrying out gordon euryale seed tissue culture rooting seedling domestication and transplanting; Instantly breaking dormancy of the gordon euryale seeds, removing seed shells and ectosperm of the seeds, and conducting germination acceleration on embryos by using a tissue culture technology, so that obtaining gordon euryale seedlings; completely establishing the gordon euryale seed sterile rapid propagation system from seed primary generation to tissue culture rooting seedling transplanting; according to the method, euryale ferox seed dormancy can be broken at any time throughout the year for the first time, and constraints of seasons on research, development and production of Euryale ferox are thoroughly break through.

Description

technical field [0001] The invention relates to a method for in vitro rapid propagation of Gorgon fruit. Background technique [0002] Gorgon fruit (Euryale ferox Salisb.) belongs to the Nymphaeaceae Euryale genus and is an annual large-scale aquatic herb. In Southeast Asia, it is mostly distributed in lakes, ponds and beaches. Gorgon is listed as one of the "Suzhou Water Eight Immortals", and it is also a high-quality authentic medicinal material in Suzhou. [0003] Gorgon contains a lot of protein, calcium, phosphorus, iron, fat, starch, vitamin B1, vitamin B2, vitamin C, crude fiber, carotene, amino acids, etc., containing as many as 18 kinds of amino acids, including threonine, phenylalanine, Lysine and other amino acids necessary for the human body not only have high nutritional value, but also have therapeutic effects such as nourishing blood and calming the nerves, nourishing the kidney and strengthening essence, removing dampness and invigorating the spleen, and st...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/00Y02P60/40
Inventor 问涛李吉春曲爱爱
Owner 江苏绿冶苗木科技有限公司
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