Culture solution for improving yield of stem cell exosomes

A technology for culture medium and stem cells, which is applied in the direction of cell culture active agent, culture process, animal cells, etc., can solve the problems of synergy, no disclosure of exosome production, no disclosure, etc., and achieves improved production and good application prospects. , the effect of low cost

Active Publication Date: 2021-11-02
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the process does not disclose the yield of exosomes obtained, and the process does not give any inspiration on how to make the components in the cell culture medium play a synergistic effect on increasing the yield of exosomes

Method used

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  • Culture solution for improving yield of stem cell exosomes
  • Culture solution for improving yield of stem cell exosomes
  • Culture solution for improving yield of stem cell exosomes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1: Preparation of umbilical cord mesenchymal stem cell culture medium of the present invention

[0059] The preparation steps of 1L umbilical cord mesenchymal stem cell culture medium are as follows:

[0060] Add 100mL serum substitute and 20mL fetal bovine serum to 880mL DMEM / F12 basal medium. After the color of the medium is stable, add 10ng interleukin-6, phosphorylated MARCKS Peptide10mmol, 5μmol Quercetin D5, and mix well to obtain, 4 Store at ℃.

Embodiment 2

[0061] Embodiment 2: Preparation of umbilical cord mesenchymal stem cell culture solution of the present invention

[0062] The preparation steps of 1L umbilical cord mesenchymal stem cell culture medium are as follows:

[0063] Add 110mL of serum substitute and 10mL of fetal bovine serum to 880mL of DMEM / F12 basal medium. After the color of the medium is stable, add 1ng of interleukin-6, 1mmol of phosphorylated MARCKS Peptide, 1μmol of Quercetin D5, and mix well to obtain, 4 Store at ℃.

Embodiment 3

[0064] Embodiment 3: Preparation of umbilical cord mesenchymal stem cell culture medium of the present invention

[0065] The preparation steps of 1L umbilical cord mesenchymal stem cell culture medium are as follows:

[0066] Add 70mL of serum substitute and 50mL of fetal bovine serum to 880mL of DMEM / F12 basal medium. After the color of the medium is stable, add 100ng of interleukin-6, phosphorylated MARCKS Peptide 20mmol, 10μmol of Quercetin D5, and mix well to obtain, 4 Store at ℃.

[0067] The following is the preparation of the control culture medium.

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Abstract

The invention discloses a cell culture fluid for improving the yield of stem cell exosomes, and belongs to the technical field of cell culture. The culture solution comprises the following components: a component 1: a basic culture solution; component 2: serum and / or serum substitute; component 3: inflammatory factors; component 4: active polypeptide; and a component 5: a cell signal channel inhibitor. The culture solution provided by the invention can promote the formation of vesicles of the umbilical cord mesenchymal stem cells, and the yield of exosomes of the umbilical cord mesenchymal stem cells is further improved by activating the activity of the SIRT1 gene. In the culture solution, the interleukin-6, the phosphorylated MARCKS Peptide and the Quercetin D5 play a synergistic interaction role in improving the yield of the umbilical cord mesenchymal stem cell exosome. The culture solution provided by the invention has the advantages of easily available raw materials, simple preparation method and low cost, and has a good application prospect in preparation of high-yield exosomes.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a culture solution for increasing the output of stem cell exosomes. Background technique [0002] Exosomes originate from the vesicles of late endosomes (also known as multivesicular bodies, MVB), which are multivesicular bodies containing multiple small vesicles formed by the inward depression of the endocytic vesicle membrane. The multivesicular body fuses with the cell membrane and releases into the extracellular matrix to become a membranous vesicle with a diameter of about 30-100 nm. Exosomes were first discovered by Johnstone et al. during the study of the transition from reticulocytes to mature erythrocytes. Later studies found that not only reticulocytes can release such small vesicles, but almost all types of living cells can secrete exosomes. [0003] Exosomes contain various biologically active substances such as DNA fragments, mRNA, small RNA, funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0665C12N2500/90C12N2501/2306C12N2501/999C12N2501/998
Inventor 申重阳
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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