Dual-gate organic electrochemical transistors

Abstract

The invention provides a transistor and a method for detecting and / or determining the concentration of an analyte in a sample by using the transistor. The transistor comprises: a source electrode; a drain electrode; a channel comprising an organic semiconductor between the source electrode and the drain electrode; a plurality of gate electrodes; and an electrolyte, wherein the electrolyte contacts the gate electrodes and the channel, and wherein at least one gate electrode comprises an oxidoreductase and at least one gate electrode does not comprise an oxidoreductase, and methods for detecting and / or determining the concentration of an analyte in a sample using the transistor comprising: (i) applying a voltage to the at least one gate electrode which does not comprise an oxidoreductase; (ii) contacting the test sample in the transistor; (iii) applying the voltage used in (i) to the at least one gate electrode which comprises an oxidoreductase, (iv) removing the voltage from the at least one gate electrode which does not comprise an oxidoreductase; wherein (iii) and (iv) occur simultaneously.

Description

technical field [0001] The present invention relates to the field of biosensors in general. More specifically, the present invention relates to organic electrochemical transistor (OECT) based biosensors and methods of using the same. Background technique [0002] The following discussion of the background of the invention is provided merely to assist the reader in understanding the invention and is not intended to describe or constitute an admission that prior art to the invention exists. [0003] Current methods for detecting the presence and / or determining the concentration of an analyte in a sample are generally complex and expensive. Sensitivity often comes at the expense of low selectivity and sometimes slow operation. [0004] Mass spectrometry is a common analytical technique in which a sample is ionized and the ions are then separated according to their mass-to-charge ratios. Detection of ions is performed by a device capable of detecting charged particles, such a...

AI Technical Summary

Problems solved by technology

However, the reaction time of OECT is slower

Due to the interference effect at the gate electrode, OECT-based biosensors also suffer from poor selectivity; for example, the channel current of OECT is affected by the ion concentration of the analyte solution, which is difficult to control in practical applications

When this happens, the channel current response induced by the target analyte cannot be selectively obtained

[0010] There is an unmet need for highly sensitive, selective, low-cost, rapid and / or simple methods / devices that can be used to detect and / or quantify specific analytes within a sample

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