Culture method and oscillation device of mesenchymal stem cell factor essence
A technology of stem cells and essence, which is applied in the field of culturing method of stem cell factor essence and its oscillation device, which can solve the problems of cell clusters and bottle walls falling off, difficult manpower to beat the bottle walls, etc.
Pending Publication Date: 2021-11-09
ANHUI HUIEN BIOTECH
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Problems solved by technology
[0003] Aiming at the deficiencies of the prior art, the purpose of the present disclosure is to provide a method for culturing the stem cell factor essence and its oscillation device, which solves the problem of the prior art that the cell clusters and the bottle wall are difficult to fall off and the bottle wall is knocked by manpower
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[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present disclosure with reference to the accompanying drawings in the embodiments of the present disclosure. Apparently, the described embodiments are only some of the embodiments of the present disclosure, not all of them. Based on the embodiments in the present disclosure, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present disclosure.
[0021] Such as Figure 1-5 As shown, a kind of vibration device of the stem cell factor essence liquid comprises a base plate 1, said base plate 1 is provided with a first installation groove 11, and the two sides of the first installation groove 11 are provided with a second installation groove 12, and the base plate 1 is provided with a first installation block 2, and both sides of the first installation block 2 are provided with support rods ...
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The invention discloses a culture method and an oscillation device of a mesenchymal stem cell factor essence, and belongs to the field of cell culture oscillation devices. The oscillation device comprises a bottom plate, a first mounting groove is formed in the bottom plate, second mounting grooves are formed in the two sides of the first mounting groove, a first mounting block is arranged on the bottom plate, supporting rods are arranged on the two sides of the first mounting block, the supporting rods are movably mounted in the second mounting grooves, a second mounting block is arranged on the first mounting block, a first rotating disc is arranged on the bottom plate, a cam groove is formed in the first rotating disc, a third mounting block is movably mounted in the first mounting groove, a first stand column is mounted on the third mounting block, a first spring is arranged between the third mounting block and the first mounting groove, a fourth mounting block is arranged on the first stand column, a second spring is arranged between the fourth mounting block and the first mounting block, the upper end of the first stand column is movably mounted in the cam groove, and the first rotating disc rotates to drive the second mounting block to reciprocate, so that the culture device is vibrated, and cell clusters are separated from the bottle wall.
Description
technical field [0001] The disclosure belongs to the field of cell culture oscillating devices, and in particular relates to a method for cultivating a stem cell factor essence and an oscillating device thereof. Background technique [0002] There are usually two ways of cell culture growth, one is suspension culture; the other is adherent culture. At this stage, the culture of umbilical cord mesenchymal stem cells is an adherent culture method. After the umbilical cord mesenchymal stem cells are stripped, the cells need to be cultured in a standard 75 culture flask. Because the cells have the characteristics of adherent growth, the mesenchymal stem cells are all attached to the inner wall of the culture flask. During the cell extraction process, trypsinization is required, and then the adherent cells are detached from the culture flask wall by beating the culture flask. Because the cells have adherent growth, the ability to adhere to the wall is determined by the volume o...
Claims
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Login to View More IPC IPC(8): C12M1/10C12M1/00C12M3/00
CPCC12M27/16C12M27/10
Inventor 熊志军刘文刘嘉慧董欣
Owner ANHUI HUIEN BIOTECH