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Choroid plexus organoids and methods for production thereof

An organoid, choroid plexus technology, applied in the field of generating choroid plexus organoids, which can solve problems such as poorly understood availability

Pending Publication Date: 2021-11-09
UK RES & INNOVATION LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The choroid plexus is an important entry point for drugs into the CNS; however, little is known about the regulation and permeability of B-CSF-B and how it can be used as a tool to increase the availability of certain drugs in the brain

Method used

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  • Choroid plexus organoids and methods for production thereof
  • Choroid plexus organoids and methods for production thereof
  • Choroid plexus organoids and methods for production thereof

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Embodiment 1

[0264] As previously reported, cerebral organoids are characterized by intrinsic self-organization, with recent protocols yielding predominantly forebrain features (13, 14), which also include ChP epithelium (Fig. 7A) (9, 13). Therefore, to study the development of human ChPs, we established a protocol based on a brain organoid approach to generate ChPs in a reliable and reproducible manner.

[0265] The derivation of ChP cells in vitro using the backing factor Bmp4 alone (15) and in combination with the Wnt activator molecule CHIR (16) has been previously reported.

[0266] Therefore, to promote ChP fate in brain organoids, Bmp4 and CHIR were given as pulses starting on day 10 of the modified brain organoid protocol (see Methods) (Fig. 1A, 7B, 7C) (7, 9, 13) . At day 14, non-committed forebrain organoids developed large, rounded neuroepithelial lobes, whereas in Bmp4 / CHIR-treated organoids, we observed more elongated neuroepithelial organization (Fig. 7B). This observation ...

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Abstract

Methods and materials relating to cultured choroid plexus organoids comprising: (a) an epithelium comprising a tight epithelial barrier; and / or (b) one or more cysts surrounded by an epithelium, plus other authentic features and markers.

Description

[0001] field of invention [0002] The present invention relates to choroid plexus organoids, particularly choroid plexus organoids comprising a tight epithelial barrier and capable of producing cerebrospinal fluid-like fluid. The present invention also relates to methods of producing choroid plexus organoids. Background technique [0003] The choroid plexus is a highly conserved and understudied secretory tissue in the brain. This tissue expands in humans in proportion to the size of our brains, where it exhibits many important functions, such as forming a protective epithelial barrier and secreting cerebrospinal fluid (CSF) (1). [0004] Anatomically, the choroid plexus is located in the lateral, third, and fourth ventricles emerging from each corresponding cavity. The mature choroid plexus consists of a single cuboidal epithelium surrounding a core of stromal tissue of mesenchymal origin and fenestrated capillaries. Epithelial cells are closely connected by tight junctio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/079C12N5/0793G01N33/50
CPCC12N2501/415C12N2501/155C12N5/0697C12N5/0619C12N5/0618C12N2502/081C12N2502/08C12N2506/02C12N2506/45C12N2533/90G01N33/5082C12N2533/54C12N2503/02
Inventor M·A·兰开斯特L·佩莱格里尼
Owner UK RES & INNOVATION LTD
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