Plant extract, preparation method thereof and application of plant extract as plant growth regulator

A technology for plant growth regulators and plant extracts, which is applied in the directions of plant growth regulators, plant growth regulators, botanical equipment and methods, etc., can solve the problems of low seedling rate, difficulty in rooting, and unestablished tissue culture system. , to achieve the effect of improving rooting rate, easy access and shortening germination time

Active Publication Date: 2021-11-19
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still problems such as rooting difficulty and low seedling rate in establishing peony regeneration system by direct rooting from embryos, and a complete tissue culture system has not yet been established.

Method used

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  • Plant extract, preparation method thereof and application of plant extract as plant growth regulator
  • Plant extract, preparation method thereof and application of plant extract as plant growth regulator
  • Plant extract, preparation method thereof and application of plant extract as plant growth regulator

Examples

Experimental program
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Effect test

preparation example Construction

[0030] The first aspect of the present invention provides a method for preparing a plant extract, characterized in that the method comprises the following steps:

[0031] (1) Obtain the blade of the rooted plant and mash it to obtain a paste;

[0032] (2) Extracting the paste with an extraction solvent and filtering to obtain the plant extract as a filtrate.

[0033] In some preferred embodiments, the method further comprises the step of sterilizing the filtrate after filtering.

[0034] In some more preferred embodiments, the method further includes the step of subpackaging the extracted plant extracts.

[0035] In some more preferred embodiments, the method further includes the step of storing the plant extract at a temperature below -20°C.

[0036]In some more preferred embodiments, the weight ratio of the paste to the extraction solvent is 1:50 to 1:200 (eg 1:100 or 1:150), more preferably 1:100.

[0037] In some more preferred embodiments, the leaching solvent is water...

Embodiment 1

[0064] Embodiment 1: the preparation of plant extract

[0065] Cut the young leaves of the ground-rooted plants of Crassulaceae, weigh 100mg of fresh leaves with a balance, put them into a 1.5mL centrifuge tube, use a grinding rod to mash and grind them into a paste, and then transfer them to a 50mL centrifuge tube filled with 10ml sterile water. tube (see figure 1 ), placed at 4°C for 12 hours, using a syringe and a circular mixed fiber filter membrane with a diameter of 100 mm and a filter hole diameter of 0.22 μm in a clean bench to filter and sterilize the plant extracts and distribute them into sterile 1.5 mL centrifuge tubes Then put it into -20℃ refrigerator for long-term storage.

Embodiment 2

[0066] Example 2: Functional Verification of Plant Extracts

[0067] 1. Materials and methods

[0068] 1.1 Materials

[0069] In this embodiment, mature seed embryos of the tree peony variety 'Fengdan' (Paeonia ostii 'Fengdan') were used. 'Fengdan' grows in the resource garden of the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences in Beijing, China. Collect mature 'Fengdan' seeds in autumn and store them at room temperature for subsequent use

[0070] 1.2. Method

[0071] The seeds were rinsed under tap water for 10 minutes, then washed with detergent (Liby), and finally rinsed in tap water to remove external stains on the seeds. Then, soften the seeds overnight (about 12 hours) by soaking in clean water, and select the plump seeds that sink to the bottom of the pot. Disinfect the mature seeds with 75% alcohol for 1 minute in an ultra-clean workbench, rinse them with sterile distilled water for 3-5 times, then disinfect them with 2%...

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PUM

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Abstract

The invention provides a plant extract, a preparation method thereof and an application of the plant extract as a plant growth regulator. Specifically, the invention provides the preparation method of the plant extract. The preparation method comprises the following steps that leaves of an air plant are obtained and mashed to obtain paste; the paste is extracted using an extraction solvent and filtered to obtain the plant extract as a filtrate. The invention further provides a plant growth regulator containing the plant extract, a rooting culture medium containing the plant extract and an application of the plant extract in the aspect of promoting rooting, for example, a peony tissue culture method adopting the plant extract. According to the plant extract, the preparation method thereof and the application of the plant extract as the plant growth regulator, materials of the plant extract are obtained easily, the preparation method is simple, special equipment and organic solvents are not needed, pollution, toxicity and side effect are not provided, and the hypocotyl germination time can be shortened obviously, rooting is promoted, the rooting rate is improved, and the elongation and growth of roots are promoted.

Description

technical field [0001] The present application relates to the field of plant growth regulators, in particular to a plant extract and its preparation method and application. Background technique [0002] IBA is often used for root induction in plant tissue culture, and is sometimes used in combination with IAA or NAA to induce rooting. In addition, acetylsalicylic acid has also been applied to induce rooting in plant tissue culture. For example, Xia Haiwu used acetylsalicylic acid and aspirin to increase the rooting rate of cabbage test tube seedlings. h 2 o 2 It has the effect of breaking dormancy on a variety of plants. Among these plant growth regulators, except that gibberellins are directly extracted from organisms, most of them are chemically synthesized, and most of them also have certain toxicity (Wang Xiaolan, 2005. Safety of plant growth regulators in crop production Sexual Use. Journal of Gansu Radio and Television University, 15(3): 1-4.). Since there is no e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01N65/08A01N43/38A01N59/00A01P21/00
CPCA01H4/001A01H4/008A01N65/00A01N65/08A01N43/38A01N59/00
Inventor 王顺利刘蓉薛璟祺张秀新
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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