Extracorporeal animal replacement evaluation method for evaluating anti-aging effect of cosmetic raw materials

An evaluation method and technology of cosmetics, applied in the direction of cosmetics, cosmetic preparations, dressing preparations, etc., can solve the problems of no clear efficacy evaluation method, and achieve the effect of wide evaluation application field, small individual, and easy operation

Inactive Publication Date: 2021-11-26
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, many anti-aging cosmetic raw materials have been launched one after another, but there is no clear efficacy evaluation method to evaluate the improvement of cosmetic raw materials on the relative collagen content in the skin

Method used

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  • Extracorporeal animal replacement evaluation method for evaluating anti-aging effect of cosmetic raw materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Effects of GHK-copper tripeptide on collagen in the epidermis of Caenorhabditis elegans irradiated by UVA band:

[0019] Collect synchronous Caenorhabditis elegans at the L4 stage, prepare NGM medium coated with OP50 uracil-deficient Escherichia coli, the formula of NGM medium is: 975mL deionized water, 17g agar, 2.5g peptone, 3g NaCl, 1M concentration Potassium phosphate buffer 25mL, 1M MgSO 4 1 mL, 1M CaCl 2 1 mL, 1 mL of cholesterol at a concentration of 5 μg / mL, pH 6.0. ). Pick Caenorhabditis elegans onto NGM medium and divide into four groups, each group has three plates of medium, and each plate has 500 nematodes. The first group and the second group are administration groups, the GHK-copper tripeptide concentration is 10 μ M, the first group medium GHK-copper tripeptide natural growth group, the second group medium GHK-copper tripeptide UVA irradiation group, and the second group medium GHK-copper tripeptide UVA irradiation group Three groups of media blank...

Embodiment 2

[0021] Effects of GHK-copper tripeptide on collagen in the epidermis of Caenorhabditis elegans irradiated by blue light band:

[0022] Collect synchronous Caenorhabditis elegans in the L4 stage, prepare NGM medium coated with OP50 uracil-deficient Escherichia coli, the formula of NGM medium is: 975mL deionized water, 17g agar, 2.5g peptone, 3g NaCl, 1M concentration Potassium phosphate buffer 25mL, 1M MgSO 4 1 mL, 1M CaCl 2 1 mL, 1 mL of cholesterol at a concentration of 5 μg / mL, pH 6.0. ), pick Caenorhabditis elegans onto the NGM medium, divide them into four groups, each group has three plates of medium, and each plate has 500 nematodes. The first group and the second group are administration groups, the GHK-copper tripeptide concentration is 10 μ M, the first group medium GHK-copper tripeptide natural growth group, the second group medium GHK-copper tripeptide blue light irradiation group, the second group medium GHK-copper tripeptide blue light irradiation group, the s...

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Abstract

The invention discloses an extracorporeal animal replacement evaluation method for evaluating the anti-aging effect of cosmetic raw materials. According to the method, caenorhabditis elegans is taken as a model organism, and the influence change of functional cosmetic raw materials on hydroxyproline indexes in epidermal collagen is examined, so that the skin anti-aging effect (including light aging) of cosmetics is evaluated. The method comprises the following steps: respectively culturing the adult caenorhabditis elegans in the same period on the functional cosmetic raw materials, irradiating the functional cosmetic raw materials under ultraviolet rays, detecting the hydroxyproline level relative content of nematode epidermis, and comparing with a control group, so as to evaluate the improvement effect of the anti-aging functional cosmetic raw materials on the collagen of the nematode epidermis. The biological method adopted by the invention can more accurately reflect the improvement effect of the functional cosmetic raw materials on the epidermal collagen, the evaluation range of the functional cosmetic raw materials is wide, and the optimal effective concentration of the functional cosmetic raw materials can be quickly screened. The evaluation method disclosed by the invention is simple to operate and short in required period.

Description

technical field [0001] The invention relates to an in vitro animal substitution evaluation method for evaluating the anti-aging effect of cosmetic raw materials, which belongs to the technical field of cosmetics. Background technique [0002] Collagen widely exists in microorganisms and human body, and plays an important role in many aspects, including tissue architecture, cell adhesion, cell migration, tumor, angiogenesis, tissue morphogenesis and tissue repair. Collagen is best known as a long-form fiber in the extracellular matrix as the major tension-resistant component of vertebrate tissues such as tendons, cartilage bones, and skin. Collagen, the main component of the dermis and the most abundant protein in the body, provides strength and support to the skin. With the aggravation of aging, the collagen protein in the human skin loses more, and the degree of wrinkle that the epidermis loses support also intensifies. The collagen content in human skin is directly relat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/64A61Q19/08A01K67/033
CPCA61K8/64A61Q19/08A01K67/033
Inventor 章苏宁王本露
Owner SHANGHAI INST OF TECH
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