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Sonneratia caseolaris fruit endophytic fungus and application thereof in preparation of antitumor active compound

A technology for endophytic fungi, anti-tumor drugs, applied in anti-tumor drugs, microorganism-based methods, active ingredients of heterocyclic compounds, etc.

Active Publication Date: 2021-11-30
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, there are only a small number of reports on the chemical constituents of the genus Nutella, in which the structure types of compounds obtained are rich and diverse, including dimeric α-alkyl butyrolactone compounds with novel skeletons, lactone A, pentacyclic Triterpenoids, sesquiterpenoids, 5,8 dioxycycloergosteroids and lignans

Method used

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  • Sonneratia caseolaris fruit endophytic fungus and application thereof in preparation of antitumor active compound
  • Sonneratia caseolaris fruit endophytic fungus and application thereof in preparation of antitumor active compound
  • Sonneratia caseolaris fruit endophytic fungus and application thereof in preparation of antitumor active compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Strain cultivation of endophytic fungus HSG11-9

[0028] Preparation of seed medium: glucose 20g, peptone 2g, yeast extract 2g, coarse sea salt 2.5g, water 1.0L, evenly divided into two 1000mL Erlenmeyer flasks, extinguished at 120°C for 25 minutes.

[0029] Insert the HSG11-9 strain of the endophytic fungus Sangria sinensis into the prepared seed medium, and cultivate it at a constant temperature at 26°C for 3 days to obtain the seed culture solution;

[0030] (2) Fermentation of endophytic fungus HSG11-9

[0031] Preparation of fermentation medium: glucose 1.1kg, peptone 100g, yeast extract 100g, sea salt 125g, water 50L, evenly distributed in 100 1000mL Erlenmeyer flasks, extinguished at 120°C for 25-30 minutes.

[0032] Take an appropriate amount of the seed culture solution (10 mL / bottle) obtained in step (1) and put it into an Erlenmeyer flask containing a fermentation medium, and culture it at a constant temperature of 26° C. for 45 days to obtain a fermente...

Embodiment 2

[0042] (1) Strain cultivation of endophytic fungus HSG11-9

[0043] Prepare seed culture medium (10.0L): 1.5% glucose (percentage by weight, the same below), 0.5% yeast extract, 0.1% peptone, 0.11% coarse sea salt, and the rest is water; evenly distributed in 16 1000mL conical flasks, 120 ℃ for 25-30 minutes.

[0044] Inoculate the prepared seed culture medium with the endophytic fungus HSG11-9 of Sorrel fruit, and cultivate it at a constant temperature of 26°C for 3 days to obtain the seed culture solution;

[0045] (2) Fermentation of endophytic fungus HSG11-9

[0046] Preparation of fermentation medium (100L): glucose 1.6% (weight percentage, the same below), yeast extract 0.5%, peptone 0.1%, coarse sea salt 0.11%, and the rest is water; averagely distributed in 200 1000mL conical flasks, 120 ℃ Off for 30 minutes.

[0047] Take an appropriate amount of the seed culture solution obtained in step (1) and put it into an Erlenmeyer flask containing a fermentation medium, and...

Embodiment 3

[0053] (1) Strain cultivation of endophytic fungus HSG11-9

[0054] Prepare seed culture medium (1.0L): 3.0% glucose (percentage by weight, the same below), 0.1% yeast extract, 0.5% peptone, 0.6% coarse sea salt, and the rest is water; evenly distributed in three 500mL conical flasks, 120 ℃ for 25-30 minutes.

[0055] Insert the HSG11-9 strain of the endophytic fungus Sangria sinensis into the prepared seed medium, and cultivate it at a constant temperature of 28°C for 3 days to obtain the seed culture solution;

[0056] (2) Fermentation of endophytic fungus HSG11-9

[0057] Preparation of fermentation medium (10L): 3.5% glucose (weight percentage, the same below), 0.1% yeast extract, 0.5% peptone, 0.6% coarse sea salt, and the rest is water; evenly distributed in 20 1000mL Erlenmeyer flasks, 120°C Off for 25 minutes.

[0058] Take an appropriate amount of the seed culture solution obtained in step (1) and put it into an Erlenmeyer flask containing a fermentation medium, an...

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Abstract

The invention relates to a sonneratia caseolaris fruit endophytic fungus and application thereof in preparation of an antitumor active compound. A preservation number of the endophytic fungus is CGMCC No. 23226. The endophytic fungus is classified and named as Aspergillus terreus. The endophytic fungus can be used for simultaneously preparing anti-tumor compounds 1-3 as shown in the abstract.

Description

technical field [0001] The invention belongs to the field of fungal active secondary metabolites, and in particular relates to an endophytic fungus of mulberry fruit and its application in the preparation of antitumor active compounds. Background technique [0002] According to the IUCN standard, among the 20 species of endangered mangrove plants in my country, 4 species of red olive plum, Hainan sea mulberry, ovale leaf mulberry and Laspei mangrove belong to the critically endangered level. But at present, there are only a small number of reports on the chemical constituents of the genus Nutella, in which the structure types of compounds obtained are rich and diverse, including dimeric α-alkyl butyrolactone compounds with novel skeletons, lactone A, pentacyclic Triterpenoids, sesquiterpenoids, 5,8 dioxycycloergosteroids and lignans. The present invention provides an endophytic fungus (HSG11-9) and its application in the preparation of active antitumor compounds. Contents...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C12P17/04C07D493/04C07D307/68A61K31/37A61K31/366A61K31/365A61P35/00C12R1/66
CPCC12P17/181C12P17/04C07D493/04C07D307/68A61P35/00C12R2001/66C07B2200/07Y02A50/30
Inventor 邓鹏飞罗由萍马凯贤李梦甜方娇娇武伟杰
Owner HAINAN NORMAL UNIV