Method for determining endocrine disrupter in organism

A technology of endocrine disruptors and measurement methods, which is applied in the field of water pollution monitoring, can solve problems such as the decline of the nutritional structure of the ecosystem, achieve low detection limits, improve measurement accuracy, and broaden the measurement range.

Pending Publication Date: 2021-11-30
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

EDCs may also have selective effects on specific trophic-leve

Method used

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  • Method for determining endocrine disrupter in organism
  • Method for determining endocrine disrupter in organism
  • Method for determining endocrine disrupter in organism

Examples

Experimental program
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Effect test

Embodiment 1

[0087] In this embodiment, the above-mentioned method is used to measure the content of endocrine disruptors in the Pearl River aquatic organisms, specifically including:

[0088] (1) Sampling in the Guangzhou section of the Pearl River, specifically including:

[0089] 1) Plankton: Repeated trawling with 160 μm pore size nylon mesh, sieving, rinsing with Milli-Q to remove suspended matter, and repeated sieving until a sufficient number of plankton samples were collected in the mesh bag. The plankton were collected and concentrated in glass bottles and then freeze-dried.

[0090] Benthic animals: Shrimp and crabs in streams are collected synchronously during the electrofishing process, and shrimp cages are placed in deep-water river sections to collect shrimps and crabs. Live benthic animals of a certain size were collected and picked out by a mud sampler, washed with distilled water, counted and identified, and soft tissues were selected for freeze-drying and grinding.

[0...

Embodiment 2

[0105] The sample recovery rate, detection limit and quantification limit of the assay method in the present invention were investigated.

[0106] Among them, the standard was added in parallel 4 times during the determination of the recovery rate, and the spiked sediment sample was the white muscle of tilapia collected from Liuxihe Reservoir. 4-NP was spiked at 1000 ng / g and other EDCs were spiked at 100 ng / g. Measured separately and calculated the recovery rate.

[0107] The limit of detection (LOD) and limit of quantitation (LOQ) were calculated using 4 spiked samples of tilapia with a concentration of 5 ng / g, and the standard deviation (Standard derivation, SD) of each compound was calculated , then 3 times SD is LOD, and 10 times SD is LOQ. The recoveries and detection limits of the seven EDCs are shown in Table 3.

[0108] Table 3 Recovery rate and detection limit of EDCs

[0109]

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Abstract

The invention discloses a method for determining an endocrine disrupter in an organism. The method comprises the following steps of: (1) taking a water body biological sample, and mixing the water body biological sample with an internal standard substance to obtain a mixture; (2) extracting the mixture by adopting an extraction solvent to obtain an extract; (3) dissolving the extract, and filtering the solution obtained by dissolving to obtain a solution to be detected; and (4) determining the solution to be detected by using a liquid chromatography tandem mass spectrometer to obtain the content of the endocrine disrupter in the solution to be detected, enabling the endocrine disrupter to comprises estrone, estradiol, bisphenol A, octyl phenol, nonyl phenol, triclosan and triclocarban. By implementing the method, the content of the endocrine disrupter in the aquatic organism can be accurately and efficiently measured, and a good data basis is provided for subsequent effective control of the content of the endocrine disrupter in the water body.

Description

technical field [0001] The invention relates to the technical field of water body pollution monitoring, in particular to a method for measuring endocrine disruptors in organisms. Background technique [0002] Endocrine Disrupting Chemicals (EDCs) refer to a kind of exogenous substances, which have high estrogen activity, and micro-exposure can cause endocrine imbalance in organisms; in addition, EDCs can also affect the nervous system, The immune system, etc. produce toxic effects, causing the survival ability of aquatic organisms to decline. Due to the medium-strong lipophilicity of EDCs, in addition to direct toxicity to exposed organisms, they can also accumulate and latent in organisms. When this cumulative effect reaches a certain level, it can cause various toxicities such as oxidative damage and carcinogenesis to organisms. The damage cannot be eliminated in the short term, and may even be irreversible. This cumulative toxic effect can also be transmitted through bi...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 王团团王旭王赛邵帅金玉蝶
Owner HAINAN UNIVERSITY
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