Earthworm protein EDA-R activating factor

Abstract

The invention discloses an earthworm protein EDA-R activating factor. The earthworm protein EDA-R activating factor is characterized in that the earthworm protein EDA-R activating factor is a protein composed of an amino acid sequence as shown in SEQ ID No. 6, and the earthworm protein EDA-R activating factor comprises the following components: F-O-1-HM-23 with the molecular weight of 1310 + / - 100 Daltons. The protein does not have the activity of directly degrading fibrin, but has the effect of remarkably enhancing in-vivo fibrinolysis by activating EDA-R residues in vivo, thereby promoting thrombolysis. The preparation method of the earthworm protein EDA-R activating factor comprises the following steps: cleaning earthworms, carrying out nano crushing, hydrolyzing, centrifugal separation, dialyzing, gel chromatography, and electrophoretic separation and purification to obtain active components. As the molecular weight of the earthworm protein EDA-R activating factor is only about 1 / 20 of that of lumbrukinase, the earthworm protein EDA-R activating factor has strong pharmacokinetic advantages, is fast to be absorbed by oral administration and fast to take effect during injection, has a bidirectional regulation effect without side effects of bleeding and prolonged blood coagulation time, and has a wide application prospect, good economic value and social benefits.

Description

technical field [0001] The invention relates to the technical field of biochemical pharmacy, in particular to an earthworm EDA-R activator. Background technique [0002] In the prior art, ground dragon protein is used for the treatment and prevention of thrombus. In 1986, Japanese scientist Mei Yuanheng first applied modern biotechnology methods to extract thrombolytic active ingredients in earthworms. It was also confirmed that oral earthworm crude protein extract is effective in dissolving thrombus, preventing thrombus formation and reducing blood viscosity. And separated six protease components, applied for a patent - US 4,568,545. It is considered that the active component of thrombolysis is one or a combination of these six components. The six protease components are: [0003] F-O-HM-45 molecular weight: 24,500±2,000 Daltons. [0004] F-I-1-HM-54 molecular weight: 27,500±2,000 Daltons. [0005] F-I-2-HM-15 molecular weight: 27,000±2,000 Daltons. [0006] F-II-HM-6...

AI Technical Summary

Problems solved by technology

[0010] The object of the present invention is to provide a kind of earthworm protein EDA-R activator, reasonably and effectively solve the problem that the molecular weight of six earthworm protease components in the prior art is too large, poor oral absorption, slow injection onset, direct thrombolytic effect Strong, problems causing bleeding tendency and prolonged clotting time