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Micro-fluidic chip for efficient drug screening

A microfluidic chip and drug technology, applied to laboratory containers, biochemical equipment and methods, tissue cell/virus culture devices, etc., can solve the problem of increasing the length of the flow channel, reducing the concentration gradient formation efficiency, and drug loss Increase and other problems, to achieve the effect of reducing the number of layers of the flow channel structure, improving the efficiency of drug screening, and reducing the loss of drugs

Active Publication Date: 2021-12-07
SUZHOU HEALTH COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some defects in this structure: on the one hand, it realizes the formation of multiple concentration gradients through multiple layers of "Christmas trees". For each additional layer, 1-2 flow channel units will be added in this layer, thereby increasing 1- 2 concentration gradients, for example, the first layer generally has 3 flow channel units, so it has 3 concentrations, and the second layer has 4-5 flow channel units, so it has 4-5 concentrations, by adding flow channel units Increase the number of concentration gradients, but the principle of its structure is to add several concentrations by mixing several solutions that cannot be concentrated in the upper layer. In order to ensure a more uniform concentration gradient, the number of flow channel units added to each layer cannot exceed a certain number, so , in order to obtain more concentration gradients, more layers will be required
This results in lengthy flow paths and inefficient concentration gradient generation
On the other hand, in each flow channel unit, a meandering flow channel structure (such as an S-shaped flow channel structure) is usually used to achieve the mixing of the two liquids. The number of layers is high and the flow channel units are many, resulting in the entire flow channel There are many circuitous channel structures in the structure, which will greatly increase the resistance of the channel and double the length of the channel, which will further reduce the flow velocity and the formation efficiency of the concentration gradient, and will also cause drug adhesion. Increased drug loss due to attachment to flow channel walls

Method used

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  • Micro-fluidic chip for efficient drug screening
  • Micro-fluidic chip for efficient drug screening
  • Micro-fluidic chip for efficient drug screening

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] In this embodiment, the width range of the channels in the first distribution channel group 302 is 50-1500 μm, and the depth range is 2-20 μm; the width range of the channels in the second distribution channel 3040 is 30-200 μm, and the depth range is 2-20 μm. 25 μm.

[0102] In one embodiment, the numbers are sequentially numbered from top to bottom, and the volumes of the 1st to 3rd first distribution channels 3020 are U 0 , 3U 0 、9U 0 , the volumes of the three second distribution channels 3040 connected after each first distribution channel 3020 are V from top to bottom 0 , 1.5V 0 , 2V 0 , that is, the volume ratio of the three second distribution channels 3040 in each second distribution channel group 304 is Among them, U 0 and V 0 Both can represent a unit volume; and in this embodiment, the channel lengths in the first distribution channel group 302 and the second distribution channel group 304 are equal, so the above volume ratio is also equal to the are...

Embodiment 2

[0116] refer to Figure 8-10 , in this embodiment, the first distribution cavity 301 includes a first V-shaped cavity section 3010 and a first rectangular cavity section 3011 that communicate with each other, the first V-shaped cavity section 3010 communicates with the drug inlet, and the first rectangular cavity section 3011 communicates with the upstream of the first distribution channel 3020;

[0117] The second distribution cavity 303 includes a second V-shaped cavity segment 3030 and a second rectangular cavity segment 3031 that communicate with each other, the second V-shaped cavity segment 3030 communicates with the downstream of the first distribution channel 3020, and the second rectangular cavity segment 3031 communicates with the first distribution channel 3020. The upstream of the two distribution channels 3040 is connected.

[0118] Through the arrangement of the V-shaped chamber section, the solution in the distribution chamber can be more evenly distributed thr...

Embodiment 3

[0129] refer to image 3 , Figure 11-14 , the end of the third mixing channel 45 has a first flaring section 47, and the first flaring section 47 communicates with the side of the cell culture chamber 50; by setting the first flaring section 47, it can facilitate the rapid entry of liquid into the cell culture chamber 50 .

[0130] A one-way valve 48 is disposed in the third mixing channel 45 , and the one-way valve 48 is located between the outlet end of the fourth mixing channel 46 and the first flaring section 47 . The one-way valve 48 acts as a non-return valve. By setting the one-way valve 48, it is ensured that the candidate drug solution after the concentration is configured can smoothly enter the cell culture chamber 50, and the culture fluid or cells in the cell culture chamber 50 will not flow backward toward the chip body. 1 upstream flow.

[0131] refer to Figure 11-12 , in one embodiment, the one-way valve 48 includes a sealing piece 485 and a limiting block...

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Abstract

The invention discloses a micro-fluidic chip for efficient drug screening. The chip comprises a chip body and a micro-channel structure formed in the chip body, and the micro-channel structure comprises a concentration gradient generation unit located at the upstream of the chip body, a cell sampling unit located at the downstream of the chip body, a mixing unit communicated with the downstream of the concentration gradient generation unit, and a cell culture unit communicated between the mixing unit and the cell sampling unit. According to the invention, through the design of the flow channel volume, the flow of the candidate medicine in unit time has a certain gradient, the prepared liquid keeps the same amount, and the two solutions can form a series of candidate medicine solutions with a series of concentrations after being mixed. Uniform mixing of the two solutions does not need to be considered in the process of forming the flow gradient of the candidate medicine, so that the method is not limited to a traditional layer-by-layer increasing mode, and the number of layers of the flow channel structure can be greatly reduced.

Description

technical field [0001] The invention relates to the field of drug screening, in particular to a microfluidic chip for efficient drug screening. Background technique [0002] In the process of new drug research and development, drug screening is to adopt appropriate methods for substances that may be used as drugs, detect their possible pharmacological activities, and provide experimental data for the development of new drugs. Appropriate drug screening strategies can improve the efficiency of screening and shorten the life cycle of new drugs. Development cycle. [0003] The high-throughput drug screening technology based on microfluidic chips is now widely used in the preliminary screening of drugs due to its fast and efficient characteristics. Microfluidic chips have many unique advantages in cell-level drug screening, such as the amount of cells and drugs required for microfluidic chip operations, which are suitable for the research of scarce cells or expensive drugs; mic...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M3/00
CPCB01L3/5027C12M23/16B01L2200/10B01L2300/0861
Inventor 刘松柏常宏
Owner SUZHOU HEALTH COLLEGE