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MOR detection kit

A detection kit and kit technology, applied in the field of kits, can solve the problems of low accuracy of liver cancer, inability to diagnose, detect and treat as soon as possible, so as to avoid low accuracy, improve the use effect, and improve the accuracy Effect

Pending Publication Date: 2021-12-10
PEKING UNIV THIRD HOSPITAL +1
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  • Description
  • Claims
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Problems solved by technology

[0006] In order to make up for the deficiencies of the existing technology and solve the problem that the accuracy rate of the detection of liver cancer in the existing technology is not high; it cannot accurately diagnose, discover and treat the liver cancer in the early stage of the patient, the present invention proposes a MOR detection kit

Method used

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Embodiment approach

[0034] As an embodiment of the present invention, a storage plate 9 is slidably connected to the bottom of the kit body 1; during operation, the present invention places ice cubes in the storage plate 9 by moving the storage plate 9, so that the preparation process is carried out on ice cubes. It is convenient to control the temperature of the detection reagent inside the kit body 1, thereby ensuring the activity of the enzyme and primer inside the detection reagent; then observe the level of MOR gene expression to reflect the strength of the proliferation ability of liver cancer cells, and the observed results Accuracy is higher.

[0035] As an embodiment of the present invention, the fluorophore primers used in the qPCR method also include the shMOR-1 gene; when working, the present invention adds the fluorophore primers corresponding to the shMOR-1 gene to the detection reagent, To detect the expression of the shMOR-1 gene in the blood sample, using the shMOR-1 gene has the...

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Abstract

The invention belongs to the technical field of kits, and particularly relates to an MOR detection kit. The MOR detection kit comprises a kit body, the upper end of the kit body is rotationally connected to a kit cover in a damping manner, the detection kit further comprises a preparation module and a moving module, the preparation module comprises a placing frame and a placing groove, the upper end of the kit body is fixedly connected to a placing rack, the placing groove is formed in the middle of the placing frame, a storage groove is formed in the rear end of the placing frame, the moving module is arranged at the upper end of the preparation module, the kit body is used for detecting by adopting a qPCR method, wherein a marker used by the qPCR method is CD133 protein, and a primer of a fluorophore used in the qPCR method is an MOR gene. According to the MOR detection kit, the problems that in the prior art, an alpha fetoprotein method is low in liver cancer detection accuracy, and the early-stage liver cancer of a patient cannot be accurately diagnosed, found and treated as soon as possible is solved.

Description

technical field [0001] The invention belongs to the technical field of kits, in particular to a MOR detection kit. Background technique [0002] Cancer is a disease caused by the loss of normal regulation and excessive proliferation of body cells, also known as malignant tumor; liver cancer is a cancer that occurs in the liver. The liver is the largest organ in the human body, located below the right lung and consists of two liver lobes. Since there are no obvious symptoms in the early stage of liver cancer, most patients have already developed to the middle and late stages when they are diagnosed, which increases the difficulty of treatment. [0003] The alpha-fetoprotein technology detection that prior art adopts, detects with alpha-fetoprotein (AFP) detection test paper (colloidal gold method) and alpha-fetoprotein (AFP) chemiluminescent method; It is only 60-70%, and there are false positives among them; so it is not possible to accurately diagnose, discover and treat ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886B65D25/10B65D25/02
CPCC12Q1/6886B65D25/10B65D25/02C12Q2600/158C12Q2600/112C12Q2600/118
Inventor 李义李翀郭向阳张旭李刚张小青王纪莲刘畅
Owner PEKING UNIV THIRD HOSPITAL
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