A method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons

Abstract

The invention relates to the field of cell culture, in particular to a method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons. The invention provides a human dopaminergic neuron induction method and a related culture solution formula. The culture medium includes serum-free basal medium, cell additives, neurodevelopmental factors and inhibitors. Among them, the use of serum-free basal medium can avoid the introduction of exogenous substances and reduce the risk of contamination. At the same time, a variety of nutrients, such as cell additives and neurodevelopmental factors, can stimulate iPSC cells to activate iPSC cells and promote differentiation. The cell culture method provided by the invention is simple, efficient, low in cost, and can induce high-quality human dopaminergic neuron cells in a short period.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons. Background technique [0002] Parkinson's Disease, also known as "Parkinson's Disease", is a common senile neurodegenerative disease. At present, there are more than 3 million patients in my country, and the number of patients ranks first in the world, accounting for Parkinson's disease in the world. More than half of the total number of patients. The characteristic motor symptoms of Parkinson's disease include resting tremor, bradykinesia, muscle rigidity, and postural balance disorders, etc., in addition, non-motor symptoms such as constipation, olfactory disturbance, sleep disturbance, autonomic dysfunction, and mental and cognitive impairment symptom. At present, it is clear that the occurrence of Parkinson's disease is related to the degeneration and death of dopaminergic n...

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Problems solved by technology

However, these surgeries have significant side effects and can lead to permanent cognitive impairment

[0003] The above-mentioned drugs used to treat Parkinson's disease basically have relatively large side effects, including dry mouth, bitter mouth, gastrointestinal discomfort, abnormal defecation, dizziness, nausea, orthostatic hypotension, ankle edema, sleep disturbance, and mental symptoms such as mental illness. Blurring, disturbance of consciousness, hallucinations, delusions, etc., which greatly reduce the quality of life of patients

Although surgical treatment can improve the symptoms of Parkinson's disease, it only has a good effect on motor symptoms such as limb tremor and muscle rigidity, and has no obvious effect on somatic axial symptoms such as posture and gait disorders, and drugs are still needed after surgery. Therefore, many patients are unwilling or unsuitable for surgery

To sum up, most of the existing drug and surgical treatments for Parkinson's disease are aimed at solving the symptoms caused by the loss of dopaminergic neurons. Reversal of neurodegeneration does not fundamentally solve the problem of degeneration and death of dopaminergic neurons

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