A method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons

A pluripotent stem cell and dopaminergic technology, applied in the field of human brain dopaminergic neurons derived from induced pluripotent stem cells, can solve problems such as lowering the quality of life, large side effects, and treating symptoms rather than the root cause, and achieves reduced pollution risks and mature functions , the effect of high purity
CN113789301BActive Publication Date: 2022-05-10广州瑞臻再生医学科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
广州瑞臻再生医学科技有限公司
Publication Date
2022-05-10

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Abstract

The invention relates to the field of cell culture, in particular to a method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons. The invention provides a human dopaminergic neuron induction method and a related culture solution formula. The culture medium includes serum-free basal medium, cell additives, neurodevelopmental factors and inhibitors. Among them, the use of serum-free basal medium can avoid the introduction of exogenous substances and reduce the risk of contamination. At the same time, a variety of nutrients, such as cell additives and neurodevelopmental factors, can stimulate iPSC cells to activate iPSC cells and promote differentiation. The cell culture method provided by the invention is simple, efficient, low in cost, and can induce high-quality human dopaminergic neuron cells in a short period.
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Description

technical field

[0001] The invention relates to the field of cell culture, in particular to a method for preparing high-purity induced pluripotent stem cell-derived human brain dopaminergic neurons. Background technique

[0002] Parkinson's Disease, also known as "Parkinson's Disease", is a common senile neurodegenerative disease. At present, there are more than 3 million patients in my country, and the number of patients ranks first in the world, accounting for Parkinson's disease in the world. More than half of the total number of patients. The characteristic motor symptoms of Parkinson's disease include resting tremor, bradykinesia, muscle rigidity, and postural balance disorders, etc., in addition, non-motor symptoms such as constipation, olfactory disturbance, sleep disturbance, autonomic dysfunction, and mental and cognitive impairment symptom. At present, it is clear that the occurrence of Parkinson's disease is related to the degeneration and death of dopaminergic n...

Claims

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