Electrochemiluminescence biosensor for detecting ulinastatin

A technology of electrochemistry and luminescent biology, which is applied in the field of biosensors, can solve the problem of low sensitivity, achieve good application prospects, simple preparation method, and low detection limit

Pending Publication Date: 2021-12-28
YANTAI UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are few methods used to detect ulinastatin, mainly using enzyme labeling method and radioactive iodine labeling method. These two methods have relatively high requirements for equipment and detection conditions, but the sensitivity is not high.

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  • Electrochemiluminescence biosensor for detecting ulinastatin
  • Electrochemiluminescence biosensor for detecting ulinastatin
  • Electrochemiluminescence biosensor for detecting ulinastatin

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Embodiment Construction

[0037] Hereinafter, the present invention is specifically described in conjunction with the accompanying drawings and specific embodiments.

[0038] Ulinastatin for detecting the ECL biosensor according to the present invention, which is prepared by the following method comes:

[0039] Step 1: obtaining a titania nanotube arrays (TiO 2 NTs)

[0040] Obtained by anodic oxidation TiO 2 NTs, includes the following steps:

[0041] (1) washing the titanium sheet

[0042] First, successively cutting each titanium sheet good 5min washed in ethanol and ultrapure water, to remove impurities from the surface of the titanium sheet. Subsequently, the titanium sheet in HF: HNO 3 : H 2 O = 1: 4: 5 (volume ratio) mixed acid solution is continued ultrasound 1 ~ 5min. Thereafter, the titanium sheet successively in ethanol and water each ultrasound 5min. Finally, the dried titanium sheet alternate with nitrogen.

[0043] (2) anodization

[0044] Take a clean titanium plate section (1cm × 0.7cm), imme...

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Abstract

The invention discloses an electrogenerated chemiluminescence biosensor for detecting ulinastatin, the electrogenerated chemiluminescence biosensor is a sandwich-type sensor, a titanium sheet is used as a basis, and the titanium sheet is directly oxidized and then is respectively combined with Ab1, ulinastatin and Ab2 for response. The preparation method comprises the following steps: obtaining TiO2NTs, preparing Ab2 / CC / MNPs, preparing an Ab2 / CC / MNPs / Ab1 / TiO2NTs electrode, and constructing the electrochemiluminescence biosensor for detecting ulinastatin. The electrogenerated chemiluminescence biosensor has the beneficial effects that the prepared electrogenerated chemiluminescence biosensor has the remarkable advantages of low detection limit, high sensitivity, good anti-interference performance and the like on ulinastatin, and has a very good applying prospect in the aspect of immunoassay.

Description

Technical field [0001] The present invention relates to a biosensor, particularly to a method for detecting the ulinastatin ECL biosensors, biosensor technical field belongs. Background technique [0002] UTI in urine known as statins (Ulinastatin, UTI), a protease inhibitor is contained in human urine, by a number of amino acids, exhibiting a wide range of enzyme inhibition. UTI by inhibition of various proteins, sugars and lipolytic enzymes, which can effectively protect the capillary permeability and vascular endothelium, the role of microcirculation. Currently, UTI is widely used in China, Korea, Japan and India. A lot of compelling evidence that UTI can not only reduce the release of pro-inflammatory cytokines, and coronary heart disease, valvular heart disease, congenital heart disease patients undergoing cardiac surgery has an important role in protecting organs. [0003] Currently, for detecting UTI are seldom, mainly ELISA method and radioiodinated method, two methods ar...

Claims

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Application Information

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IPC IPC(8): G01N21/76G01N27/26G01N27/30
CPCG01N21/76G01N27/26G01N27/30
Inventor 岳喜典田春媛王丽华情柳晓玲于京华
Owner YANTAI UNIV
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