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Streptomyces amritsarensis S1-12 and application thereof to prevention and control of soil-borne diseases

A soil-borne disease and Streptomyces technology, applied in the field of microorganisms, can solve the problems of unstable control effect, difficult to replace chemical fungicides, unsatisfactory vegetable disease control effect, etc., to achieve the effect of regulating structure and diversity, and strong adaptability

Pending Publication Date: 2022-01-14
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem that the existing microorganisms have unsatisfactory effect on the disease prevention of facility vegetables, the control effect is unstable, and it is difficult to replace chemical fungicides, the present invention provides a Streptomyces amritsarii S1-12 and its application in preventing and controlling soil-borne diseases , the Streptomyces amritsarii S1-12 has broad-spectrum disease resistance characteristics, is suitable for facility agricultural soils containing complex pathogenic bacteria, and is conducive to the healthy and green production and processing of modern facility vegetables

Method used

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  • Streptomyces amritsarensis S1-12 and application thereof to prevention and control of soil-borne diseases
  • Streptomyces amritsarensis S1-12 and application thereof to prevention and control of soil-borne diseases
  • Streptomyces amritsarensis S1-12 and application thereof to prevention and control of soil-borne diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 separation of strains

[0025] The tomato tomato of Shandong Shouguang is more than 20 years, the tomato is sampled, diluted 10 6 Double, take 1 mL of the No. 1 solid medium plate, repeating 50 plates, and picked the streptoma colony according to the form of colony, and the linear is purified.

[0026] The strain 16S RDNA sequence (GenBank: OK626407) was submitted to NCBI BLAST for similarity comparison, and the similarity of Streptomyces amritsarensis model strain 2A was 99.86%, combined with new morphology, identified the strain as Am Streptomyces Amritsarensis.

Embodiment 2

[0027] Example 2 Antibacterial Test

[0028] (1) Rhizoctonia Solani, Fusarium Oxysporum, Pythium Aphanidermatum, Fusarium Solani, Daxia (Verticillium Dahliae) ), Botrytis Cinerea, PHTOPHORACAPSICI and Alterna Solani, inoculated 2 days on PDA medium.

[0029] (2) Take the moss with a sterilized diameter of 5 mm in the edge of each pathogenic fungus, and mount the Na medium having a diameter of 9 cm, and the Amrit is picked up. Take a little time (6 cm) of the patients (6 cm) and 25 ° C, each treatment was repeated 3 times per treatment, and a control of only the pathogen.

[0030] (3) When the protocols to be controlled, the radius of the respective colonies is measured when the bacterial bacterial bacterial bacterial bacterial bacilliases is measured, and the inhibition rate is calculated. figure 1 , Table 1 shows that the S1-12 of the Amrit is broad-spectrum antibacterial activity to several spectrous antibacterials for several trial disease.

[0031] Table 1 Confarution of the A...

Embodiment 3

[0034] Example 3 Preparation of Fermentation

[0035] (1) Inoculate the Amrit of the S1-12 inoculation to the activation of Garish 1.

[0036] (2) Transfer the activated Amrit of Amrit to the fermentation medium, the fermentation temperature is controlled in the range of 28-30 ° C, 180 r / min oscillates to cultured to an effective live bacteria ≥ 10 × 10 8 CFU / g, the fermentation broth is obtained.

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Abstract

The invention relates to the technical field of microorganisms, in particular to streptomyces amritsarensis S1-12 and application thereof to prevention and control of soil-borne diseases, the streptomyces amritsarensis S1-12 is preserved in China General Microbiological Culture Collection Center on October 12, 2021, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC NO.23578. The strain is classified and named as Streptomyces amritsarensis, and the strain is named as Streptomyces amritsarensis. Streptomyces amritsarensis S1-12 has a broad-spectrum disease-resistant characteristic, is suitable for facility agriculture soil containing complex pathogenic bacteria, and is beneficial to healthy and green production and processing of modern facility vegetables.

Description

Technical field [0001] The present invention relates to the field of microbiological technology, and in particular to the application of Amrits, S1-12 and its prevention and control of native diseases. Background technique [0002] Because of the high cost of economic value, the facility vegetables is high in high sets of high sets, high reaches index, high fertilizer, pesticide application volume, resulting in decrease in soil fertility, microbial ecological imbalance, resulting in Fusarium SPP.), Phytophthora (phytophthora SPP.), Rhizoctonia SPP.), Botrytis SPP.), Pythium SPP.) and Rhizham SPP.), Alternaria SPP.) and other pathogens Set, causing the disease that is increasing, usually there are several or more diseases or alternate, prevention and treatment, have to rely on frequent use of chemical pesticides to prepare a broad spectrum of chemical pesticides, causing the pesticide residue and pathogenic bacteria to be increasingly serious, forming a vicious circle. [0003] Mi...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCC12N1/20A01N63/28Y02A40/10
Inventor 张广志张新建吴晓青周方园谢雪迎赵晓燕范素素周红姿
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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