Method for producing triploid oysters through interspecific hybridization of Fujian oysters and Crassostrea sikamea

A technology of Kumamoto oyster and triploid is applied in the field of Fujian oyster and Kumamoto oyster interspecific hybridization to produce triploid oyster, which can solve the problem of low survival rate or success rate, inability to obtain tetraploid Fujian oyster, and inability to carry out optimization and breeding. interbreeding and other issues to achieve the effect of high maturity

Active Publication Date: 2022-01-21
青岛前沿海洋种业有限公司
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Problems solved by technology

[0005] The tetraploid Fujian oyster has four sets of chromosomes, which can be obtained by fertilizing the ovum of the triploid oyster with normal diploid sperm and inhibiting the release of polar bodies (CN100415085C, CN107494358A, CN109730008A, CN113016673A), and currently produce tetraploid Fujian oyster The induction method has the limitations of instability, low survival rate or success rate, cannot obtain enough tetraploid Fujian oysters, cannot c

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Embodiment 1

[0023] The invention discloses a method for producing interspecific hybrid triploid oysters of Fujian oyster and Kumamoto oyster, which includes obtaining excellent tetraploid Fujian oysters, parent selection, parent breeding, artificial insemination, hatching and larvae cultivation.

[0024] 1) Acquisition of tetraploid Fujian oysters: In February 2019, 120 2-year-old artificially induced triploid Fujian oysters and 50 2-year-old diploid Fujian oysters were collected and placed in a salinity of 32‰, respectively. Diploid Fujian oysters were cultivated in a broodstock breeding tank with a temperature of 16°C according to the conventional method, and the cultivation method of triploid Fujian oysters was as follows: first, let stand for one day, then raise the temperature by 5°C every day from the second day until the third day reached 26°C, keep it at 26°C for 5 days, from the 8th day, lower the water temperature to 23°C, feed 8 times a day, pour the pond and change the water 10...

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Abstract

The invention relates to a method for producing triploid oysters through interspecific hybridization of Fujian oysters and Crassostrea sikamea, and belongs to the technical field of aquaculture variety genetic breeding. The method comprises the steps of obtaining of excellent tetraploid Fujian oysters, strong optimization of parents, parent cultivation, artificial insemination and incubation and larva cultivation. According to the method, the female parent of the crassostrea sikamea and the male parent of the tetraploid Fujian oyster are hybridized to obtain the triploid crassostrea sikamea, so that on one hand, the problems of small size and low growth speed are solved on the basis of keeping fine and delicious taste of the crassostrea sikamea, and on the other hand, through the characteristic of high sterility of the triploid, the crassostrea sikamea-Fujian hybrid triploid oysters which can be marketed all year round are obtained.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding of aquaculture varieties, and in particular relates to a method for producing triploid oysters by interspecific hybridization of Fujian oysters and Kumamoto oysters. Background technique [0002] Fujian oyster (Crassostrea angulata), also known as Portuguese oyster, belongs to the family Ostridae and belongs to the genus Crassostrea. It is mainly distributed in the high-temperature and high-salt sea area south of the Yangtze River in my country, mainly in Fujian Province. It has the advantages of fast growth and high yield. , is an important species of oyster culture in my country. [0003] Kumamoto oyster (Crassostrea sikamea), which belongs to the Ostridae family and belongs to the genus Crassostrea, is mainly distributed in the famous seas of Japan, the Korean Peninsula and the central and southern waters of my country, suitable for high temperature, medium and high salinity sea areas, ...

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Application Information

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IPC IPC(8): A01K61/54A01K61/17
CPCA01K61/54A01K61/17Y02A40/81
Inventor 郭希瑞李雅林郭小雨魏颖
Owner 青岛前沿海洋种业有限公司
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