SNP Molecular Markers Related to Luffa Growth and Its Application

A molecular marker, loofah technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve problems affecting loofah breeding and other issues

Active Publication Date: 2022-08-02
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, complex environmental factors and genetic variation affect the selection of loofah varieties with different lengths

Method used

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  • SNP Molecular Markers Related to Luffa Growth and Its Application
  • SNP Molecular Markers Related to Luffa Growth and Its Application
  • SNP Molecular Markers Related to Luffa Growth and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Example 1 Location of genes related to the length of loofah gourd

[0098] 1. Experimental materials

[0099] Short loofah 93075 comes from: the loofah inbred line bred by the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences.

[0100] Long loofah WJ55 comes from: the loofah inbred line bred by the Vegetable Research Institute of Guangdong Academy of Agricultural Sciences.

[0101] The above-mentioned short loofah 93075 and long loofah WJ55 are shown in the figure figure 1 shown.

[0102] 2. Experimental steps

[0103] 2.1 The short loofah 93075 and the long loofah WJ55 were crossed to obtain F1, and then self-crossed to obtain F2, which was planted in a greenhouse. After 50 days, the length of each individual melon was investigated, and 3 commercial melons were investigated for each individual plant, and the average was taken. The result is as figure 2 As shown, the frequency of fruit length of loofah is a normal distribution, indicating ...

Embodiment 2

[0111] Example 2 Application of M4962 molecular marker as a molecular marker for the length of loofah gourd

[0112] 1. M4962 molecular marker and / or M4962 primer set is used to identify or assist in the identification of luffa length traits

[0113]Thirty-five loofah strains were planted in Baiyun Base, Zhongluotan, Baiyun District, Guangzhou, under normal water and fertilizer management. After 50 days, the melon length of each loofah variety was investigated (as shown in Table 3). Using the M4962 primer set to identify the melon length traits of 35 loofah lines: extract the loofah genomic DNA, use the genomic DNA as a template, and use the M4962 primer set for PCR amplification. The amplification reaction system is shown in Table 1, and the reaction conditions are shown in Table 2. After the PCR is completed, use the TECAN infinite M1000 microplate reader to read the fluorescent signal, and then use the online software snpdecoder (http: / / www.snpway.com / snpdecoder / ) to analyz...

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Abstract

The invention relates to the technical field of biological molecular markers, and discloses a SNP molecular marker related to the growth of loofah and its application. The SNP molecular marker is located at the 151st position of the nucleotide sequence as shown in SEQ ID NO.1, the polymorphism is A / G, and the molecular marker is significantly related to the length of loofah: it can be used to identify or assist in the identification of pending Measure the melon length character of the loofah, identify or assist in identifying whether the fruit of the loofah to be tested is a long or short melon, select the loofah with the long melon, and select the loofah with the short fruit and the loofah breeding.

Description

technical field [0001] The invention belongs to the technical field of biological molecular markers, and in particular relates to a SNP molecular marker related to the growth of loofah and its application. Background technique [0002] Loofah is an important multifunctional vegetable. Luffa fruit is rich in vitamins, amino acids, trace elements and antioxidants, and has the functions of clearing away heat and relieving summer heat, stopping bleeding and reducing inflammation. In recent years, with people's attention to nutrition and health, the sown area of ​​loofah has increased year by year, and it has become an important daily consumption vegetable. [0003] The fruit length of loofah commercial fruit is an important appearance quality trait. Due to different consumption habits and uses, the requirements for the length of loofah vary from place to place. According to the consumption habits of different regions, cultivating loofah varieties with different melon lengths ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 赵钢军吴海滨罗剑宁龚浩李俊星郑晓明刘小茜
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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