Microbial composition for synergistically degrading cyanophenyl herbicides and microbial agent produced from microbial composition

A technology of microbial composition and herbicide, which is applied in the field of biotechnology, can solve problems such as threats to the environment and human health, and achieve the effects of maintaining human health, good effect, and strong environmental adaptability

Active Publication Date: 2022-01-28
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented inventor describes how we can create an effective way that uses different types of chemical compounds called substances like 2-(chloromethylthio)-N'-cyanobiphenylacecticam ("2CMB"), 4HQO-3BPhenacetone oxime hydrazone," trisodermolum phosphorus pentadecaulonimbus," tetrahedronazolin-8a," chelating agents made from nitrogen gas donors such as ammonium salicylic acid), etc., together with specific enzymes found naturally occurring there during decomposition processes. These chemically synthesized decomposers have been shown to be highly efficient when used alone but are less effective compared to traditional methods due to their ability to break down harmful organisms. By combining these techniques into one system they could help reduce environmental impact without compromising beneficial properties associated with each type of treatment agent.

Problems solved by technology

This patented technical solution describes how halobionaphthalene chloronan esters were found effective treatments against harmful substances like phytotoxics or fumigans from crops. These compositions contain highly safe ingredients without causing negative side impacts upon humans and environments around us due to their low solubility in aqueous systems overtime. Additionally, this technology provides potential benefits through its ability to break down harmless materials into less dangerous products called volatile salts.

Method used

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  • Microbial composition for synergistically degrading cyanophenyl herbicides and microbial agent produced from microbial composition
  • Microbial composition for synergistically degrading cyanophenyl herbicides and microbial agent produced from microbial composition
  • Microbial composition for synergistically degrading cyanophenyl herbicides and microbial agent produced from microbial composition

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Effect test

Embodiment 1

[0036] Embodiment 1, isolation and identification of bacterial strain

[0037] The present invention provides a synergistic degrading bacterial group of benzonitrile herbicides and the bacterial agent produced therefrom. The bacterial strains used are Gram-stain-positive bacteria R45 and Gram-stain-negative bacteria X-1, which are isolated from a pesticide factory in Changzhou, Jiangsu. in the soil. The specific isolation and screening methods for strains are as follows:

[0038] Take 5.0 g of the soil sample and add it to 100 ml of inorganic salt (hereinafter referred to as MM) medium containing 0.2 mM bromoxynil octanoyl (hereinafter referred to as MM), culture it on a shaker at 30°C and 150 rpm for 5 days, and transfer it to fresh In the same culture medium, four consecutive enrichment cultures were carried out. Dilute the fifth-generation enrichment solution on MM solid medium containing 1mM bromoxynil octanoyl, culture at 30°C for 4 days, pick a single colony that produ...

Embodiment 2

[0041] Embodiment 2, laboratory degradation experiment

[0042] 2.1 Growth utilization and degradation of bromoxynil by synergistic flora

[0043] High-performance liquid chromatography for the detection of bromoxynil octanoyl: take 20mL sample and add 5mL dichloromethane to extract the whole bottle, use anhydrous sodium sulfate to remove excess water in the organic phase, then take 0.25mL in a 1.5mL centrifuge tube, blow in a ventilated place After drying, add 1 mL of methanol to redissolve, filter through an organic phase filter membrane with a pore size of 0.22 μm, and detect by HPLC. Detection conditions: Shimadzu RID-10A is the high-performance liquid chromatograph; the chromatographic column is a C18 reversed-phase column with a size of 250mm×4.6mm; the column temperature is 30°C; the mobile phase is 100% methanol, and the flow rate is 1.0mL / min; the detection wavelength for 221nm and 229nm.

[0044] Detection of bromoxynil by high performance liquid chromatography: Ta...

Embodiment 3

[0057] Embodiment 3, synergistic flora degradation ability and single bacterial strain degradation ability comparison

[0058] Add 25mg / L bromoxynil and 1% LB liquid medium to a 50mL Erlenmeyer flask containing 20mL MM liquid medium, inoculate the seed liquid of strain X-1 until the initial cell concentration is OD 600 =0.3, 30°C, 160rpm shaker culture, the whole bottle of 20 MM was destructively sampled at regular intervals, extracted with an equal volume of dichloromethane, and taken up to 70h. The concentration of octanoyl bromoxynil was detected by high performance liquid chromatography (HPLC), and its degradation curve was drawn. The result is as Figure 7 As shown, strain X-1 can rapidly degrade bromoxynil octanoyl and produce bromoxynil ( Figure 7 B), in the time of 48h, bromoxynil degradation rate reaches about 90% ( Figure 7 A).

[0059] Add 25mg / L bromoxynil and 1% LB liquid medium to a 50mL Erlenmeyer flask containing 20mL MM liquid medium, inoculate the seed ...

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Abstract

The invention discloses a microbial composition for synergistically degrading cyanophenyl herbicides and a microbial agent produced by the microbial composition. The microbial composition is composed of Pseudoxanthomonas sp. X-1 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: M 20211288 and Bacillus sp. R45 with the preservation number of CCTCC NO: M 20211262. The microbial composition and the microbial agent produced by the microbial composition have a wide degradation spectrum, and can degrade various halogenated aromatic hydrocarbons such as bromoxynil octanoate, bromoxynil, 3, 5-dibromo-4-hydroxybenzoic acid, 3-bromo-4-hydroxybenzoic acid and the like. According to the invention, the pollution problem of cyanophenyl herbicides in industrial and agricultural production activities is successfully solved, so that the ecological environment is protected, and the human health is maintained.

Description

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Claims

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Application Information

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Owner NANJING AGRICULTURAL UNIVERSITY
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