Pretreatment optimization method for fish gill metabonomics sample

A technology for sample pretreatment and optimization methods, applied in the field of metabolomics, which can solve the problems of no relevant reports on metabolomics pretreatment methods

Pending Publication Date: 2022-02-01
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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Problems solved by technology

So far, there have been studies on the metabolomics pretreatment methods of biological samples such as serum, plasma, and embryos, but there are still no relevant reports on the metabolomics pretreatment methods applied to fish gill tissue samples

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  • Pretreatment optimization method for fish gill metabonomics sample
  • Pretreatment optimization method for fish gill metabonomics sample
  • Pretreatment optimization method for fish gill metabonomics sample

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention is described below through specific embodiments shown in the accompanying drawings. It should be understood, however, that these descriptions are exemplary only and are not intended to limit the scope of the present invention. The structures, proportions, sizes, etc. shown in the drawings of this specification are only used to cooperate with the content disclosed in the specification, for those who are familiar with this technology to understand and read, and are not used to limit the conditions for the implementation of the present invention. Therefore, Without technical substantive significance, any modification of structure, change of proportional relationship or adjustment of size shall still fall within the technology disclosed in the present invention without affecting the effect and purpose of the present invention. within the scope of the conten...

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Abstract

The invention discloses a fish gill metabonomics sample pretreatment optimization method, and relates to the technical field of metabonomics. The optimization method comprises the following steps: 1, preparing instruments and reagents; 2, UPLC-Q-TOF/MS data acquisition and analysis: acquisition and preparation of a sample, carrying out UPLC-Q-TOF/MS analysis, and performing data processing and statistical analysis. According to the invention, a UPLC-Q-TOF/MS technology is adopted, and a research object is a non-targeted metabonomics gill tissue sample. In the scheme, a liquid phase extraction and purification method involved in the pretreatment method is optimized and methodologically investigated, and analysis and discussion are carried out based on the coverage rate (the number of detected potential metabolites) and the stability (the RSD of the relative peak area and retention time of the metabolites); meanwhile, methodological investigation is carried out by utilizing a QC sample, and finally, the UPLC-Q-TOF/MS non-targeted metabonomics sample pretreatment method suitable for the gill tissues is established.

Description

technical field [0001] The invention belongs to the technical field of metabolomics, and in particular relates to a method for optimizing the pretreatment of fish gill metabolomics samples based on LC-Q-TOF / MS technology. Background technique [0002] Metabolomics is a science that studies the overall changes and changes of endogenous metabolites produced by biological systems after external stimuli or disturbances. Metabolites are extremely sensitive to exogenous influences, and the metabolic profile of an organism can be viewed as the terminal response of a biological system to genetic or environmental changes. Therefore, metabolomics research can be applied to identify the physiological differences between cells, tissues, organs or organisms exposed to different environmental conditions (or culture conditions), identify the marker metabolites from these differences as the identification of specific conditions, and To analyze the function of specific metabolic pathways, c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8679G01N2030/062
Inventor 孙言春姚明珠刘英杰陈中祥王鹏
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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