Method for increasing number of root nodules with nitrogenase activity at roots of leguminous crops and application of method to leguminous crops

A technology of nitrogenase and leguminous plants, applied in the direction of botany equipment and methods, applications, chemicals for biological control, etc., to achieve the effects of promoting crop production, easy operation, and improving farmland soil environment

Active Publication Date: 2022-02-08
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase the number of root nodules in crops, a lot of research work has focused on rhizobia to develop excellent rhizobia, but currently there are few that can be applied to production and play a significant role

Method used

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  • Method for increasing number of root nodules with nitrogenase activity at roots of leguminous crops and application of method to leguminous crops
  • Method for increasing number of root nodules with nitrogenase activity at roots of leguminous crops and application of method to leguminous crops

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Embodiment 1

[0020] Embodiment 1 Increase the establishment of the method for the number of nitrogenase active root nodules in peanut roots (1)

[0021] Establish a method for significantly increasing the number of root nodules with nitrogenase activity in peanut roots through the following steps

[0022] (1) Peanut pods and rhizosphere soil samples were obtained from peanut pods and rhizosphere soil samples from Guangxi peanut production areas, and strains were isolated from peanut pods or rhizosphere soil by conventional bacterial isolation methods, or strains were isolated from roots or rhizosphere soil of other crops such as soybeans, and strains were isolated by conventional 16s The rDNA method was used to identify and obtain a series of bacteria such as Bacillus amyloliquefaciens, Bacillus sporogenes, Bacillus colloidus, and Enterobacter ludwig. Then, through conventional bacterial medium amplification culture or conventional Bacillus fermentation method, the series of bacterial stra...

Embodiment 2

[0027] Example 2 Establishment of the method for increasing the number of nitrogenase active nodules in peanut roots (two)

[0028] Establish a method for significantly increasing the number of root nodules with nitrogenase activity in peanut roots through the following steps

[0029] (1) Peanut pods and rhizosphere soil samples were obtained from peanut pods and rhizosphere soil samples from Jiangsu peanut production areas, and strains were isolated from peanut pods or rhizosphere soil by conventional bacterial isolation methods, or strains were isolated from roots or rhizosphere soil of other crops such as soybeans, and strains were isolated by conventional 16s The rDNA method was used to identify and obtain a series of bacteria such as Bacillus amyloliquefaciens, Bacillus sporogenes, Bacillus colloidus, and Enterobacter ludwig. Then, through conventional bacterial medium amplification culture or conventional Bacillus fermentation method, the series of bacterial strains obta...

Embodiment 3

[0034] Example 3 Establishment of the method for increasing the number of nodules with nitrogenase activity in peanut roots (three)

[0035] Establish a method for significantly increasing the number of root nodules with nitrogenase activity in peanut roots through the following steps

[0036] (1) Obtain peanut pods and rhizosphere soil samples from the peanut production area in Hebei, and isolate strains from peanut pods or rhizosphere soil by conventional bacterial isolation methods, or isolate strains from roots or rhizosphere soil of other crops such as soybeans, and isolate strains from the roots of other crops such as soybeans or rhizosphere soil by conventional 16s The rDNA method was used to identify and obtain a series of bacteria such as Bacillus amyloliquefaciens, Bacillus sporogenes, Bacillus colloidus, and Enterobacter ludwig. Then, through conventional bacterial medium amplification culture or conventional Bacillus fermentation method, the series of bacterial str...

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Abstract

The invention relates to a method for increasing the number of root nodules with nitrogenase activity at roots of leguminous plants and application of the method to leguminous crops. After two or three of bacillus amyloliquefaciens, brevibacillus laterosporus and bacillus mucilaginosus are mixed with enterobacter ludwigii, the mixture is applied to the field of leguminous crops to increase the number of root nodules with nitrogenase activity at the roots of the leguminous crops. The method is used to increase the number of root nodules with nitrogenase activity at the roots of leguminous crops such as peanuts, and is simple to use, remarkable in yield increasing benefit and ecological benefit and easy to popularize and apply.

Description

technical field [0001] The invention relates to a method for increasing the number of root nodules with nitrogenase activity on the roots of leguminous crops and its application on leguminous crops. Background technique [0002] When legumes such as peanuts grow in the field, the roots can be infected by rhizobia and form nodules. If these nodules have nitrogenase activity, they can fix free nitrogen and synthesize nitrogen-containing compounds, which can be used by legumes to obtain high yields. , which has very important practical significance for fertilizer reduction, carbon peaking, and carbon neutralization. If the number of root nodules is relatively dense, it is called super nodulation phenomenon. Hypernodulation is still extremely rare. So, how do legumes such as peanuts produce large numbers of root nodules with nitrogenase activity? This has been a hot topic of research all over the world. [0003] In actual production, due to the long-term and large-scale use ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/22A01N63/20A01P21/00C05F11/08C05G3/00
CPCA01N63/22A01N63/20C05F11/08C05G3/00Y02P60/21
Inventor 李培武张奇岳晓凤
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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