Antibacterial peptide Cbf-14 hydrogel as well as preparation method and application thereof

A technology of cbf-14 and hydrogel, which is applied in the direction of antibacterial drugs, antifungal agents, local antibacterial agents, etc., can solve the problems of lack, reduce toxicity, etc., achieve fast sterilization speed, reduce toxic and side effects, and excellent wettability Effect

Pending Publication Date: 2022-02-25
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently a lack of topical formulations that can further reduce toxicity

Method used

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  • Antibacterial peptide Cbf-14 hydrogel as well as preparation method and application thereof
  • Antibacterial peptide Cbf-14 hydrogel as well as preparation method and application thereof
  • Antibacterial peptide Cbf-14 hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 discloses the screening of the antimicrobial peptide Cbf-14 hydrogel-forming matrix.

[0036] Commonly used water-soluble gel bases include carbomer, poloxamer, cellulose gel base, etc., choose 0.5% carbomer, 4.0% sodium carboxymethyl cellulose, 4.0% hydroxyethyl cellulose The gel matrix and 2.0% methyl cellulose were used as the gel matrix, and the gel formed by mixing the gel matrix with water was prepared, and the appearance, uniformity, spreadability and viscosity were evaluated to determine the gel matrix. The results are shown in Table 1.

[0037] Table 1. Peptide Gel Blank Matrix Screening Results

[0038]

[0039] From the results in Table 1, it can be known that choosing 2.0% mass concentration of methylcellulose can form a gel with better quality.

Embodiment 2

[0041] Example 2 discloses the screening of the antimicrobial peptide Cbt-14 hydrogel moisturizer.

[0042] Glycerin and propylene glycol were selected for the screening of moisturizers, and different moisturizers, methylcellulose and distilled water were added to prepare the gel. The water loss rate of the prepared gel was measured, and its preliminary stability (centrifugal stability, low temperature stability, heat resistance stability) was evaluated. The addition, grouping and experimental results of specific moisturizers are shown in Table 2, and the end-point water loss rates of gels prepared with different moisturizers are shown in figure 2 .

[0043] Table 2. Effect of different humectants on the preparation of gels

[0044]

[0045]

[0046] From the results in Table 2, it can be seen that choosing glycerin as the humectant in the gel helps to reduce the water loss rate and reduce the loss of water. Next, the dosage of the humectant is further screened, and t...

Embodiment 3

[0051] The antimicrobial peptide Cbf-14 hydrogel containing the following raw materials in mass percentages was prepared: 0.5% peptide Cbf-14, 10.0% glycerin, 2.0% methylcellulose, and distilled water to make up the balance.

[0052] The preparation method of hydrogel containing antimicrobial peptide Cbf-14 comprises the following steps: firstly, methyl cellulose is mixed with distilled water, left to swell at room temperature; the polypeptide Cbf-14 is dissolved, and sterilized by filtering through a 0.22 μm filter membrane; adding glycerin Stir to dissolve; mix the above components evenly, let stand to swell, and centrifuge to remove air bubbles to obtain the antimicrobial peptide Cbf-14 hydrogel. See the physical picture of the antimicrobial peptide Cbf-14 hydrogel Figure 4 , SEM image see Figure 5 .

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PUM

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Abstract

The invention discloses antibacterial peptide Cbf-14 hydrogel as well as a preparation method and application thereof. The hydrogel is prepared from the following materials in percentage by mass: 0.5 to 2.0 percent of polypeptide Cbf-14, 1.0 to 2.0 percent of methyl cellulose and10.0 to 15.0 percent of glycerol, with the balance being distilled water. The antibacterial hydrogel provided by the invention has good antibacterial activity on pathogenic microorganisms such as gram positive bacteria and gram negative bacteria, and can effectively reduce a bacterial load at the skin wound of a rat and promote the healing of the skin wound. The antibacterial peptide Cbf-14 hydrogel has a unique preparation process and a convenient administration mode, and has wide application prospects.

Description

technical field [0001] The invention relates to an antibacterial hydrogel and its preparation method and application, in particular to a preparation method and application of an antibacterial peptide Cbf-14 hydrogel. Background technique [0002] Hydrogel is a three-dimensional cross-linked network polymer material, using water as the dispersion medium, its hydrophilic residues combine with water molecules to link the water molecules inside the network, and the hydrophobic residues swell with water. The characteristics make it maintain its original structure without being dissolved after being swollen with water. Due to the good biocompatibility and biosafety performance of hydrogel, it is widely used in the fields of biological enzyme immobilization, tissue engineering, drug delivery, etc., and it is also used in non-medical fields such as environmental sanitation, memory switching elements, cosmetics, and food industry. It also has very broad application prospects. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/10A61K9/06A61K47/38A61K47/10A61P17/02A61P31/04A61P31/10A61P31/02
CPCA61K38/10A61K9/06A61K9/0014A61K47/38A61K47/10A61P17/02A61P31/04A61P31/10A61P31/02
Inventor 赵文煊刘晓芸周晨雨周长林马菱蔓
Owner CHINA PHARM UNIV
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