Intelligent microorganism wall-breaking extraction device and extraction method

Abstract

The invention relates to an intelligent microorganism wall-breaking extraction device and an extraction method. The wall-breaking extraction device comprises a fixed drum, a middle shaft, a rotary drum and an adjusting mechanism, wherein the fixed drum is vertically arranged, and the middle shaft is rotatably arranged in the fixed drum through a rotation stopping spring; the rotary drum sleeves a grinding column, a grinding space is formed between the rotary drum and the grinding column, and a liquid storage space is formed between the rotary drum and the fixed drum; the lower end of the rotary drum is connected with a circular ring table in a relatively rotating manner, and the grinding column and the rotary drum are conical with large upper parts and small lower parts; and the lower end of the adjusting mechanism is connected with the inner bottom part of the fixed drum and is matched with the middle shaft through a control rod group, and the upper end of the same is connected with the circular ring table through a temperature-sensing pressure spring. Ascending and descending of the rotary drum and the circular ring table are controlled by the adjusting mechanism, in the wall breaking bead grinding process, when the temperature of the grinding space rises or materials in the grinding space are broken, mixed liquid in the grinding space and mixed liquid in the liquid storage space are exchanged, substances in cells are prevented from being damaged at the high temperature, and meanwhile the wall breaking efficiency is improved.

Description

technical field [0001] The invention relates to the field of microbial wall-breaking extraction, in particular to an intelligent microbial wall-breaking extraction device and an extraction method. Background technique [0002] When using microorganisms, it is sometimes necessary to break the microbial cell fluid to meet the needs of experiments and use. Cell wall breaking refers to the technology of using external force to destroy the cell membrane and cell wall, so that the cell contents, including the target product components, are released. At present, there are mainly mechanical crushing methods and non-mechanical crushing methods for cell wall breaking. Mechanical crushing methods include high-pressure homogenization, bead milling, impact crushing, and ultrasonic crushing. They are widely used due to their large processing capacity, high crushing efficiency, and fast speed. Application, in the mechanical crushing method, the effect of bead milling is better. [0003] ...

AI Technical Summary

Problems solved by technology

[0004] According to at least one deficiency of the prior art, the present invention proposes an intelligent microbial wall-breaking extraction device and extraction method to solve the problem that existing microbial wall-breaking devices are prone to heat and cause cell deterioration

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