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Method for preparing influenza ha split vaccine

A technology for vaccines and influenza, applied in biochemical equipment and methods, microbes, pharmaceutical formulations, etc.

Pending Publication Date: 2022-02-25
JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NAT INST OF INFECT IOUS DISEASES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, issues regarding production for practical application remain to be resolved, and the development of HA vaccine antigens that can more easily induce anti-stem antibodies has been expected

Method used

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  • Method for preparing influenza ha split vaccine
  • Method for preparing influenza ha split vaccine
  • Method for preparing influenza ha split vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0113] 1. Preparation of HA Split Vaccine

[0114]Add Tween 80 to H3N2 influenza virus particles (X31 strain) or H1N1 influenza virus particles (A / Puerto Rico / 8 / 34 strain) suspended in phosphate buffered saline to a final concentration of 0.1 v / v% and suspend in it . Diethyl ether was added and suspended, and the suspension was left standing until the water layer and ether layer were completely separated, and then the ether layer was removed. After repeating this ether extraction, diethyl ether remaining in the recovered aqueous layer was distilled off at normal pressure to obtain a HA split vaccine.

[0115] 2. Acid treatment

[0116] The HA split vaccine was suspended in phosphate-buffered saline, and then subjected to acid treatment by adding 0.15 M citrate buffer (pH 3.5) to bring the pH to 5.0. After standing at room temperature for 30 minutes, 1 M Tris buffer (pH 8.0) was added to restore the pH to 7.3. Thereafter, centrifugation was performed to obtain a membrane-fu...

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Abstract

Provided is a method for preparing an influenza HA split vaccine which produces an antibody that binds to an HA stem region of influenza, wherein it is difficult for the HA stem region to produce an antigenic variant. Acidic treatment is performed on the influenza HA split vaccine. By performing the acidic treatment, the influenza HA split vaccine is obtained which produces an antibody that binds to an LAH of an HA stem region. This influenza HA split vaccine has good protective ability against infection from other influenza viruses having different antigenicities.

Description

technical field [0001] The present invention relates to a method for producing influenza HA split vaccine. Background technique [0002] Current influenza hemagglutinin (hereinafter also abbreviated as "HA") vaccines induce anti-HA antibodies, thereby exerting protection against infection. Anti-HA antibodies bind to a portion of the virus called the "head region" exposed outward from the viral membrane. This region undergoes structural changes most frequently among virus strains. Thus, in some cases, anti-HA antibodies may not be able to bind a virus that is antigenically mutated and different from the vaccine strain, and the vaccine may not protect against infection. [0003] In recent years, it has been revealed that antibodies that bind to a stalk region less likely to cause antigenic variation include protective antibodies against infection (Patent Document 1). In order to efficiently induce antibodies that bind to the stem region, the HA stem protein with a stabilize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/145A61P31/16
CPCA61P31/16A61K39/12C12N2760/16134A61K2039/5252A61K2039/575A61K2039/55505A61K2039/543A61K2039/545A61K39/145
Inventor 高桥宜圣安达悠阿户学
Owner JAPAN AS REPRESENTED BY DIRECTOR GENERAL OF NAT INST OF INFECT IOUS DISEASES
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