Teaching kit for separating microorganisms for decomposing cellulose and application thereof
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A kit and microorganism technology, applied in the field of microorganism separation kits, can solve the problem of lack of regional division or related guidance process, etc.
Pending Publication Date: 2022-03-01
SOUTHWEST UNIV
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Problems solved by technology
Existing kits simply provide the reagents required for the experiment, lacking area division or related guidance for the experimental steps and reagent properties (such as difficult-to-dissolve components during the configuration process)
Method used
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Embodiment 1
[0048] Embodiments of the present invention provide a kit for isolating cellulolytic microorganisms, such as figure 1 As shown, the test kit includes a cover (1) and a box body (2); wherein, the box body (2) is composed of several separated spaces, and the spaces respectively constitute four areas for holding and combining to achieve different acting reagents. Each space is filled with plastic or foam, and the four areas are distinguished by four different colors of green, white, red, and yellow.
[0049] Such as figure 2 and image 3 As shown, with the opening and closing direction of the cover (1) and the box (2) as the front, a green cuboid space (3) is set on the left side in front of the box (2) as area A (green area) ; By the six white cuboid spaces on the right side in front of the box body (2): cuboid space (4), cuboid space (5), cuboid space (6), cuboid space (7), cuboid space (8), cuboid space Space (9) forms area B (white area); two red cuboid spaces of the sa...
Embodiment 2
[0059] The experiment of isolating cellulolytic microorganisms was carried out according to the experimental materials in Table 4 below and the kit in Example 1.
[0060] Table 4 isolates the experimental material table of the microorganisms that decompose cellulose
[0061]
[0062] Experimental steps:
[0063] (1) Open the kit and count the reagents according to the instructions.
[0064] (2) Preparation of culture medium (prepared according to the specific components and proportions shown in Table 5)
[0065] Preparation of enrichment medium: Take out all the products in the red area (area C) of the kit, add the enrichment medium-carbon source in 1000mL distilled water, and wait until it is completely dissolved by heating; then add other reagents in the red area in turn: enrichment culture Base-organic nitrogen source, enrichment medium-growth factor, after mixing evenly, take out the white area (area B) products: basic component-phosphorus source ①, basic component-ma...
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Abstract
The invention belongs to the technical field of microbial separation kits, and particularly relates to a teaching kit for separating cellulose-decomposing microorganisms and application of the teaching kit, and the teaching kit realizes a guiding function of experimental steps and experimental drug effects by utilizing partitions with different colors. The kit comprises a cover body and a box body, the box body is composed of a plurality of separated spaces; the space is filled with other materials (such as plastic or foam), and the space is divided into an area A, an area B, an area C and an area D; and a corresponding label is arranged around each space. The invention provides a kit for separating microorganisms capable of decomposing cellulose, regional planning is carried out by utilizing reagent properties, combination is carried out according to a basic amount prepared by 1000mL of a culture medium, and the workload of experiment preparation is reduced; the color and the naming guidance of the contained reagent are utilized to guide the specific operation of the experiment step by step so as to make preparation for the principle behind the clarification experiment operation, and the difficulty of experiment teaching is reduced.
Description
technical field [0001] The invention belongs to the technical field of microorganism separation kits, and in particular relates to a teaching kit for separating microorganisms that decompose cellulose and an application thereof. Background technique [0002] Microbiology-related knowledge has a wide range and a strong connection with practical applications in life. Microbial experiments can effectively play a supporting role in the observation of life phenomena and scientific associations. The operating techniques and experimental skills involved are also Microbial knowledge can be connected with life practice, based on facts, combined with scientific principles and related logical methods, and using specific ways of thinking to understand, understand, deal with and analyze life phenomena and biological problems. Coherent experiments based on the diversity of microorganisms combine experimental techniques with corresponding research on microbial functions, so as to form a sy...
Claims
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Application Information
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