Tissue culture method for colorful syngonium podophyllum
A technology for tissue culture and fruit taro, applied in the field of plant tissue culture, can solve the problems of low rooting efficiency and easy variation of the dazzling fruit taro, and achieve the effects of improving the rooting efficiency, reducing the mutation rate and having appropriate components.
Active Publication Date: 2022-03-08
FOSHAN SANSHUI YOUNGPLANTS
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[0003]
The purpose of the present invention is to provide a method for tissue culture of colorful
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[0044] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, and the schematic embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.
[0045] 1. The culture medium used in each stage of the tissue culture of colorful Heguo taro:
[0046] Inoculation medium: MS, BA 3.0mg / L, sucrose 30g / L, carrageenan 5.8g / L, pH 6.2;
[0047] Start induction medium: MS, BA 3mg / L, IAA 1mg / L, sucrose 30g / L, carrageenan 5.8g / L, pH 6.2;
[0048] Proliferation medium: MS, BA 3mg / L, IAA 0.5~1mg / L, sucrose 30g / L, carrageenan 5.8g / L, pH 6.2;
[0049] Strong seedling growth medium: MS, AC 0.2g / L, sucrose 30g / L, carrageenan 5.8g / L, pH 6.2;
[0050] Rooting induction medium: MS, AC 1g / L, sucrose 30g / L, carrageenan 5.8g / L, pH 6.2.
[0051] 2. Materials
[0052] Requirements for the female parent: strong growth, no pests and diseases, and ...
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The invention discloses a tissue culture method for colorful syngonium podophyllum. The tissue culture method comprises the following steps: acquiring an explant, disinfecting and sterilizing; inoculating and culturing the explants; starting induction culture; cutting a tissue block mass obtained by starting induction culture, and then carrying out proliferation and propagation culture, wherein a proliferation and propagation culture medium comprises the following components: MS, 3mg/L of BA, 0.5-1mg/L of IAA, 30g/L of sucrose and 5.8 g/L of carrageenan, and the pH value is 6.2; cutting a proliferation block mass obtained by proliferation and expanding propagation, and then carrying out strong seedling and seedling culture, wherein a strong seedling and seedling culture medium comprises the following components: MS, 0.2 g/L of AC, 30g/L of sucrose and 5.8 g/L of carrageenan, and the pH is 6.2; the method comprises the following steps: cutting seedlings obtained by seedling strengthening and seedling forming culture, and carrying out rooting induction culture to obtain tissue culture seedlings, wherein a rooting induction culture medium comprises the following components: MS, 1g/L of AC, 30g/L of sucrose and 5.8 g/L of carrageenan, and the pH value is 6.2. The method has the advantages that the proliferation and propagation culture medium, the strong seedling culture medium and the rooting induction culture medium are proper in component, mass proliferation culture of block seedlings can be realized, seedlings are efficiently strengthened, the rooting efficiency is improved, and the mutation rate is reduced.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to the plant tissue culture of the genus Synapsis. Background technique [0002] Synagogue belongs to the Araceae family and belongs to the perennial evergreen herbaceous plant. The leaves are dimorphic, arrow-shaped or halberd-shaped; small ear-shaped leaves are often grown on both sides of the leaf base lobes. Dazzle Color Dazzle Color, also known as Bai Fumei, belongs to the genus Syngonium Dazzle Color of Araceae. The Latin scientific name is Syngonium'Dazzle Color'. The original variety is Neon, which was independently selected by the applicant during the tissue culture process. Variations. The main color of the leaves is white, mixed with red spots, such as figure 1 shown. After being promoted and sold in the market, colorful yam taro has become more and more popular among consumers. Tissue culture is the main production method of the colorful Hego taro, but ...
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Login to View More IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02P60/40
Inventor 蒋雄詹启成黎东均张花周博
Owner FOSHAN SANSHUI YOUNGPLANTS