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Method for visually and quantitatively detecting Vc content and portable intelligent sensing system

A quantitative detection and intelligent sensing technology, applied in the direction of measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of complex instrument operation, long measurement time, cumbersome extraction procedures, etc., achieve intuitive detection process, reduce hardware cost, solve Manipulate complex effects

Pending Publication Date: 2022-03-11
HEBEI UNIVERSITY
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Problems solved by technology

[0009] The above-mentioned first four methods, due to the disadvantages of requiring cumbersome extraction procedures, complicated instrument operations, and long measurement times, have hindered their widespread application.
Although the common fluorescent sensing strategy can detect Vc sensitively and quickly, like other quantitative detection methods, it also requires professional technicians to have expensive instruments and laboratory conditions, which is usually very complicated and time-consuming, and is not suitable for online real-time detection

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  • Method for visually and quantitatively detecting Vc content and portable intelligent sensing system
  • Method for visually and quantitatively detecting Vc content and portable intelligent sensing system
  • Method for visually and quantitatively detecting Vc content and portable intelligent sensing system

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Embodiment Construction

[0049] The present invention has synthesized SiCDs using specific materials and specific processes, and the prepared SiCDs can be synthesized by Fe 3+ specifically quenched and restored by Vc, so the present invention is based on SiCDs and Fe 3+ To detect the Vc content in food.

[0050] 1. Synthesis of SiCDs

[0051] In the present invention, SiCDs are synthesized by a one-step hydrothermal method. Specifically: 0.8 g of citric acid and 0.2 g of cysteine ​​were dissolved in 8 mL of deionized water, ultrasonically dissolved for 15 min, and nitrogen gas was bubbled for 10 min to remove oxygen. Then 2 mL of 3-aminopropyltriethoxysilane (APTES) was injected into the nitrogen-saturated precursor solution. Subsequently, the prepared SiCDs precursor solution was transferred into a 25 mL polytetrafluoroethylene-lined stainless steel autoclave and incubated at 200 °C for 2 h. After cooling to room temperature, the resulting mixture was purified by dialysis bag (100-500 Da) for 24h...

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Abstract

The invention provides a method for visually and quantitatively detecting the content of Vc and a portable intelligent sensing system. The portable intelligent sensing system comprises a cassette which is installed on the back of a smart phone through an adapter. An ultraviolet lamp is arranged in the cassette, and a fluorescent test strip is fixed at the bottom of the cassette; a SiCDs + Fe < 3 + > fluorescent sensor solution is printed in a wax ring on the fluorescent test strip; during detection, a to-be-detected sample solution is dropped into the wax ring, the ultraviolet lamp irradiates an area in the wax ring, the smart phone is used for photographing and identifying the RGB value of a picture, and the corresponding data processing module is used for calculating to obtain the Vc content in the to-be-detected sample solution. According to the present invention, the on-off fluorescence sensor is ingeniously designed by using the silicon carbon quantum dots (SiCDs) to detect the Vc, the fluorescence test strip has advantages of green non-toxicity, low cost, easy storage, easy transportation, easy disposal and the like, the detection process is visual based on the smartphone, and the online real-time detection is achieved.

Description

technical field [0001] The invention relates to the technical field of Vc detection, in particular to a method for visually and quantitatively detecting Vc content and a portable intelligent sensing system. Background technique [0002] Vitamin C (Vc, also known as ascorbic acid) is a highly water-soluble component that plays an extremely important role in human health. It is important for various biological processes, such as acting as a potent antioxidant and reducing oxidative stress on Vc peroxidase substrates. In clinical practice, Vc can be used to relieve vitiligo and reduce the incidence of cancer. Vc cannot be synthesized in the human body and must be ingested through food, drugs, etc. The intake of Vc is effective for treating the common cold, scurvy, mental illness, diarrhea, cancer, AIDS and infertility. On the contrary, Vc excess can cause some symptoms, such as stomach irritation, diarrhea and urinary tract stones. Considering the important role of Vc in ou...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/643
Inventor 李春花徐啸王富丽赵悦师钰博赵昕王庭欣赵志磊
Owner HEBEI UNIVERSITY
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