Method for manufacturing tissue chip

Abstract

The invention relates to a tissue chip manufacturing method, and belongs to the technical field of tissue chips, and the method comprises the following steps: scanning a paraffin section by using a digital scanner, and obtaining a scanned and amplified scanning image; and performing microscopic marking based on the scanning image, and obtaining an effective target tissue from the wax block corresponding to the slice according to a marking result to serve as a tissue chip column core. According to the method, accurate target tissue marking is carried out based on the digital intelligent scanning section, invalid points of the constructed medium-high density tissue chip are greatly reduced, the core quality and the accuracy of the tissue chip are obviously improved, the heterogeneity difference of each point of the malignant tumor tissue chip is reduced, the freshness of cells in the target tissue is effectively guaranteed, and the application prospect is wide. Further, the accuracy of immunohistochemistry and gene research is improved. The marking work intensity of pathologists can be reduced, the tissue chip manufacturing time is shortened, and the rejection rate is reduced, so that manpower, material resources and financial resources are saved.

Description

technical field [0001] The application belongs to the technical field of tissue chips, and in particular relates to a method for making cores from tissue chips. Background technique [0002] Tissue chip (tissue chip, TC), also known as tissue microarray (tissue microarray, TMA), like gene chip, protein chip and cell chip, is a component of biochip technology and belongs to high-tech biotechnology. Tissue chip is a new type of high-throughput, multi-sample research tool that arranges dozens or hundreds of tissue samples of different individuals on a carrier according to a predetermined sequence for morphological or molecular biology experiments. Tissue microarray technology has the advantages of large amount of information, small experimental error, strong comparability, time-saving, labor-saving, and cost-saving. The combination of ISH, fluorescent nucleic acid in situ hybridization (FISH) and in situ PCR technology can realize the morphological observation of normal or dis...

AI Technical Summary

Problems solved by technology

[0004] In the process of TMA construction, pathological histological markers are always used throughout, but at present, pathological markers are mainly positioned by manual light microscope, the marking range is large, the positioning accuracy is not high, and the freshness and amount of target tissue are uncertain. , leading to unstable results of immunohistochemistry and other experiments, and false negatives and false positives occur from time to time; at the same time, there are uneven levels of existing pathological markers, and too little target tissue, too much necrosis, hemorrhage, and fibrous tissue in tissue stratification , and even the problem of many invalid points due to chip drop-off, resulting in the low quality of the chip core after core making, which will lead to unsatisfactory experimental results and waste manpower, material resources and financial resources

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