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Method for discriminating purity of BMR sorghum photo-thermo-sensitive sterile line hybrid

A technology for the purity of photothermo-sensitive sterile lines and hybrids, applied in horticultural methods, botanical equipment and methods, and plant genetic improvement, can solve problems such as inconvenient operation, long time, laborious labor, etc., and achieve improved accuracy , Strong practicability and accurate detection effect

Active Publication Date: 2022-03-25
DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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Problems solved by technology

[0006] The present invention proposes a method for discriminating the purity of BMR sorghum photothermo-sensitive male sterile line hybrids, which solves the problem of long time, laborious and inconvenient operation of the method for discriminating the purity of sorghum male sterile line hybrids in the related art. Discrimination of hybrid plants of BMR sorghum photothermosensitive male sterile line due to fertility change caused by sterile line population receiving fertile pollen

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  • Method for discriminating purity of BMR sorghum photo-thermo-sensitive sterile line hybrid

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Embodiment 1

[0028] A method for discriminating the purity of a BMR sorghum photothermosensitive male sterile line hybrid, comprising the following steps:

[0029] (1) Preparation of BMR sorghum photothermosensitive male sterile line hybrid seeds: On May 15, 2019, 3 copies of the BMR sorghum photothermosensitive male sterile line hybrid seeds to be tested were evenly selected, and the numbers were: HG389×S2006- 1. HG389×S2006-2, HG389×S2006-3, 1200 seeds per serving. The source of the hybrid is BMR sorghum photothermo-sensitive male sterile line HG389 as the female parent and Sudangrass S2006 as the male parent;

[0030] (2) Sowing of BMR sorghum photothermo-sensitive male sterile line hybrid seeds: On May 15 and May 30, 2019, 3 hybrid seeds were sown in Hengshui, Hebei, and each seed was sown in one plot. The community adopts individual plant on-demand dense planting, and each material is sown in 10 rows, with a row length of 6m, a row spacing of 50cm, and a plant spacing of 5cm;

[003...

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Abstract

The invention relates to the technical field of crop new variety breeding, and provides a BMR sorghum photo-thermo-sensitive sterile line hybrid seed purity discrimination method, which comprises: S1, abnormal plant identification: planting a BMR sorghum photo-thermo-sensitive sterile line hybrid seed F1 in a field, and if a BMR single plant appears in a population, indicating that the single plant is a sterile line selfing plant and is an abnormal plant; s2, detecting the purity of the hybrid seeds: taking to-be-detected BMR sorghum photo-thermo-sensitive sterile line hybrid seeds for sowing, after emergence of seedlings, counting the total number of group single plants and the total number of BMR single plants, and calculating the purity of the BMR sorghum photo-thermo-sensitive sterile line hybrid seeds; s3, removal of abnormal plants: in a BMR sorghum photo-thermo-sensitive sterile line hybrid field, manually pulling out BMR single plants in time. According to the technical scheme, the problems that in the prior art, a method for judging the purity of the sorghum sterile line hybrid is long in time, labor-consuming and inconvenient to operate are solved, and the problem that due to fertility change of the BMR sorghum photo-thermo-sensitive sterile line, sterile line groups receive fertile pollen and bear abnormal plants is solved.

Description

technical field [0001] The invention relates to the technical field of breeding of new crop varieties, in particular to a method for judging the purity of BMR sorghum photothermosensitive male sterile line hybrids. Background technique [0002] Sorghum can be further divided into three phenotypes according to the color of the midrib: white midrib (WMR), green midrib (GMR) and brown midrib (BMR). The color of the white midrib is white. The color of the midrib of the wax-veined type is green, while the midrib of the brown BMR type sorghum is generally brown when the plant grows to the six-leaf stage. Brown midveined BMR materials were crossed with white or wax veined materials, the hybrid F1 showed white or wax veins, but no brown midvein; but in the F2 population, the segregation ratio of non-brown midvein individual plants and brown midvein individual plants was close 3:1, indicating that the brown midrib BMR trait is a recessive trait controlled by a single gene. At the sa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/20A01G7/00A01H1/02
CPCA01G22/20A01G7/00A01H1/022
Inventor 李源赵海明游永亮武瑞鑫刘贵波
Owner DRY LAND FARMING INST OF HEBEI ACAD OF AGRI & FORESTRY SCI
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