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Cryopreservation method and device for biological materials

A biological material and cryopreservation technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of embryos popping up or knocking down, the embryos cannot be retrieved, and it is difficult to fit tightly, so as to achieve an improvement Efficiency, avoiding the risk of embryo contamination, and avoiding the effect of embryo confusion

Pending Publication Date: 2022-03-25
XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, even if the rod body and the closure cap are made of the same material, it is difficult to ensure that the two are completely tightly fitted, and there is still a risk of leakage
[0008] In addition, the carrier rods of the prior art usually do not have a fixed place for placing embryos, but are placed at a certain position on the carrier rod according to the doctor's habit, and the position of the embryo needs to be found during observation; The loading tank for embryos, however, since the loading rod is usually long, it needs to be operated carefully and stably, otherwise the embryos may be ejected or knocked down due to the tilt or vibration of the loading rod, and the embryos may not be retrieved during thawing bad results

Method used

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  • Cryopreservation method and device for biological materials
  • Cryopreservation method and device for biological materials
  • Cryopreservation method and device for biological materials

Examples

Experimental program
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Effect test

Embodiment 1

[0069] This embodiment provides a method for cryopreserving embryos such as blastocysts on day 5-6, which uses a water-in-oil droplet forming chip to prepare water-in-oil droplets that form cryopreserved biological materials. The method of the present invention is applicable to all cryopreservation methods of biological materials such as conventional freezing method, programmed freezing method and vitrification method, and the vitrification method is taken as an example to describe below.

[0070] Such as figure 1 As shown, the water-in-oil droplet forming chip 10 of the present embodiment includes a biological material circulation main pipeline, a cryopreservative branch pipeline, and an oil phase material branch pipeline: one end of the biological material circulation main pipeline is a biological material inlet 101, and the other end It is the biological material oil drop outlet 102; on the biological material circulation main pipeline, the branch pipe connection port of th...

Embodiment 2

[0081] This embodiment provides another device for cryopreserving embryos such as blastocysts on day 5-6, the structure of which is as follows: Figure 4 As shown, the cryopreservation device for biological materials includes a water-in-oil droplet forming chip 10 and a cryopreservation rod 30 . The structure of the water-in-oil droplet forming chip 10 is basically the same as that in Embodiment 1. The front end of the head of the freezing loading rod 30 is provided with a concave hole as the loading groove 301, and the opening end of the loading groove 301 is detachably sleeved on the biomaterial oil droplet outlet 102 of the water-in-oil droplet forming chip. The tail of the freezing rod 30 is a hand-held end, which is provided with a biological material sample information barcode area 302 .

[0082] The biomaterial oil droplet A formed by using the water-in-oil droplet forming chip 10 flows out from the biomaterial oil droplet outlet 102 and directly enters the loading gro...

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Abstract

The invention provides a cryopreservation method and device for a biological material. The cryopreservation method of the biological material comprises the following steps: encapsulating the biological material to be cryopreserved in an oil phase material by adopting a water-in-oil technology to form biological material oil drops; wherein the biological material comprises somatic cells, sperms, ova, D1-D6 embryos or plant cells from human or animals; the oil phase material comprises one or more of glycerol, mineral oil and vegetable oil; and performing cryopreservation on the biological material oil drops. The cryopreservation device for the biological material comprises a water-in-oil droplet forming chip. The storage sealing performance and firmness of the biological materials can be improved.

Description

technical field [0001] The present invention relates to a method and device for cryopreservation of biological materials, in particular, to a method for cryopreserving biological materials such as sperm, eggs, embryos, etc., and the cryopreservation method used in the method. Rods and forming devices such as chips. Background technique [0002] In the field of fertility technology, some biological materials such as somatic cells, sperm, eggs, embryos on day 1-6 usually need to be cryopreserved. For example, in IVF treatment, since many patients after egg retrieval are not suitable for fresh embryo implantation, fertilized eggs or blastocysts need to be frozen for implantation when the patient returns to a suitable state for implantation. In addition, in order to prevent the failure of one implantation, the temporarily unused eggs taken out at the same time are cryopreserved to prevent the occurrence of the need to take eggs again for women, etc., all of which involve the fr...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221
Inventor 丁韬力万成任军
Owner XUKANG MEDICAL SCI & TECH (SUZHOU) CO LTD
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