Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

SNP (Single Nucleotide Polymorphism) site-containing polynucleotide related to schizophrenia and application of SNP site-containing polynucleotide

A schizophrenia and polynucleotide technology, which is applied in the direction of recombinant DNA technology, biochemical equipment and methods, and microbial detection/testing, can solve the problem of not finding IL21R schizophrenia molecular markers, and achieve efficient screening, Simple operation and fast effect

Active Publication Date: 2022-04-05
SHANGHAI JIAO TONG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] A search of the existing literature only found that the position adjacent to IL21R on chromosome 16 was significantly associated with autism, but there was no report in the Chinese population that rs146177564 of IL21R was a molecular marker for schizophrenia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • SNP (Single Nucleotide Polymorphism) site-containing polynucleotide related to schizophrenia and application of SNP site-containing polynucleotide
  • SNP (Single Nucleotide Polymorphism) site-containing polynucleotide related to schizophrenia and application of SNP site-containing polynucleotide
  • SNP (Single Nucleotide Polymorphism) site-containing polynucleotide related to schizophrenia and application of SNP site-containing polynucleotide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] In the present embodiment, the screening of single nucleotide polymorphism site SNP includes as follows:

[0082]According to case-control association studies and whole-exome targeted sequencing of schizophrenia families, it was found that rs146177564 of the interleukin-21 receptor (IL21R) gene located on chromosome 16 was significantly associated with schizophrenia.

[0083] Chromosome rs146177564 is a biallelic polymorphic SNP site on human chromosome 16 DNA, rs146177564 is located in NM_021798 (GRCh37), HGVS: NC_000016.9, located in IL21R gene.

[0084] The rs146177564 site is located at position 40863 in the sequence shown in SEQ ID No.1, where the nucleotide is C or T, and when the nucleotide is T, the rs146177564 allele is T type; When the nucleotide is C, the rs146177564 allele is type C.

[0085] figure 1 The schematic diagram of the position of rs146177564 in IL21R in this example. Among them, the extracellular region of IL21R has two FNⅢ domains (the specif...

Embodiment 2

[0094] In the present embodiment, the method for detecting susceptibility to schizophrenia includes the following steps:

[0095] 2.1 Collection and processing of blood samples

[0096] 5 mL of human peripheral blood was collected in vacutainer tubes containing trisodium citrate (3.2%) and lithium heparin and allowed to stand for at least 6 hours. Invert blood collection tubes 3-5 times to ensure thorough mixing of blood and anticoagulant.

[0097] 2.2 Genomic DNA extraction and identification

[0098] DNA extraction was performed using the DNA Mini Kit (250) kit.

[0099] The DNA concentration of the extracted DNA was measured on the Thermo Fisher NanoDrop 2000 ultra-micro-volume UV spectrophotometer; when performing sample determination, it is necessary to record the three values ​​of concentration, 260 / 280 and 260 / 230, and exclude those whose concentration does not reach 10ng / μL At the same time, DNA samples should be stored in aliquots to avoid repeated freezing and tha...

Embodiment 3

[0118] In this embodiment, a kit for detecting the susceptibility to schizophrenia is provided, which includes a primer pair capable of amplifying the genomic DNA base sequence near the SNP site rs146177564 of the IL21R gene, and other PCR reagents. The kit of the present invention is stored at -20°C in the dark.

[0119] The kit contains specific primers, detection reagents and detection chips whose sequences are shown in SEQ ID NO.2, SEQ ID NO.3, and SEQ ID NO.4.

[0120] Detection reagents include PCR reaction solution, SAP enzyme reaction solution and UEP extension reaction solution. Wherein the PCR reaction solution includes ddH 2 O, PCR Buffer, heat-resistant polymerase; SAP enzyme reaction solution includes ddH 2 O, SAP Buffer and SAP enzyme; UEP extension reaction solution includes ddH 2 O, Gold Buffer, Termination mix and high temperature resistant elongase.

[0121] In the present invention, the polymorphism of IL21R gene rs146177564 is significantly correlated w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biological medicines, in particular to a schizophrenia-related polynucleotide containing an SNP (Single Nucleotide Polymorphism) site and application of the schizophrenia-related polynucleotide, and the SNP site in the separated polynucleotide is located at the 27454345th site of a human 16th chromosome. The SNP site has significant correlation with schizophrenia, and can be used for identifying the susceptible site carrying schizophrenia, detecting the susceptibility of people to schizophrenia, screening schizophrenia susceptible individuals and evaluating the risk of schizophrenia.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the polynucleotide containing SNP sites related to schizophrenia and its use. Background technique [0002] Schizophrenia (SCZ) is a chronic, disabling mental illness based on genetic and neurodevelopmental defects. It is one of the most common mental illnesses in humans, and it is also the most serious. The incidence of schizophrenia is about 0.45‰-1.5‰, with obvious differences among regions. It also has a huge negative impact on the social economy, the World Health Organization (WHO) survey shows that the total Disability Adjusted Life Years (DALYs) of schizophrenia is about 1%. In recent years, schizophrenia has been listed as the first burden of mental illness in my country. At present, clinically, its diagnosis is mainly made by psychiatrists based on the patient's clinical symptoms and manifestations, with the help of some mental illness scales. Therefore, in-depth ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6858C12N15/11
CPCY02A50/30
Inventor 贺光石毅袁凡
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com