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Bionic multichannel electroactive nerve conduit and preparation method thereof

A nerve conduit, multi-channel technology, applied in the field of biomedical materials and regenerative medicine, to achieve the effect of promoting extension and myelination process, easy in vivo transplantation, and excellent suture performance

Active Publication Date: 2022-04-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above, the present invention is aimed at the fact that current products are difficult to simulate the complex anatomical structure of peripheral nerves and the status quo of regenerative microenvironment. A multi-channel nerve conduit mimicking the anatomy of peripheral nerves with both piezoelectric and conductive dual electrical activity

Method used

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  • Bionic multichannel electroactive nerve conduit and preparation method thereof
  • Bionic multichannel electroactive nerve conduit and preparation method thereof
  • Bionic multichannel electroactive nerve conduit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] 1) Boil 30g of silk in 1.5L of 0.5wt% sodium bicarbonate solution for 30 minutes, then transfer it to 5L of deionized water, and rub it repeatedly in a water bath at 50°C until the foam becomes less. Dry in an oven at 60°C for later use.

[0035] 2) Dissolve 4g of silk thread degummed for the second time in a 9.3M lithium bromide solution in a water bath at 30°C, dialyze for 48 hours with a dialysis device with a molecular weight cut-off of 8000-14000, centrifuge at a speed of 11000g for 30 minutes, repeat twice to remove impurities, and obtain Silk fibroin solution.

[0036] 3) Dilute the silk fibroin solution to 6wt% with deionized water and pour it into the mold, close the lid tightly to ensure airtightness, place the mold at -20°C for 24 hours to freeze and concentrate, transfer the mold to -5°C for 48 hours to realize the self-crosslinking process of silk fibroin, and obtain multi-channel silk fibroin cryogel after demoulding.

[0037]4) Soak multichannel silk fi...

Embodiment 2

[0042] 1) Boil 30g of silk in 1.5L of 0.5wt% sodium bicarbonate solution for 30 minutes, then transfer it to 5L of deionized water, and rub it repeatedly in a water bath at 50°C until the foam becomes less. Dry in an oven at 60°C for later use.

[0043] 2) Dissolve 4 g of secondary degummed silk thread in 9.3M lithium bromide solution in a 30°C water bath, dialyze for 48 hours with a dialysis bag device with a molecular weight cut-off of 8000-14000, then centrifuge at a speed of 9000g for 30 minutes, repeat twice to remove impurities, Obtain silk fibroin solution.

[0044] 3) Dilute the silk fibroin solution to 6wt% with deionized water and pour it into the mold, close the lid tightly to ensure airtightness, place the mold at -20°C for 24 hours to freeze and concentrate, transfer the mold to -5°C for 48 hours to realize the self-crosslinking process of silk fibroin, and obtain multi-channel silk fibroin cryogel after demoulding.

[0045] 4) Soak multichannel silk fibroin cry...

Embodiment 3

[0050] 1) Boil 30g of silk in 1.5L of 2.0wt% sodium bicarbonate solution for 45 minutes, then transfer it to 5L of deionized water, and rub it repeatedly in a water bath at 60°C until the foam becomes less. Dry in an oven at 60°C for later use.

[0051] 2) Dissolve 4 g of secondary degummed silk thread in a 9.3M lithium bromide solution in a 60°C water bath, dialyze for 48 hours with a dialysis bag device with a molecular weight cut-off of 3000-8000, then centrifuge at a speed of 12000g for 30 minutes, repeat twice to remove impurities, Obtain silk fibroin solution.

[0052] 3) Dilute the silk fibroin solution to 2wt% with deionized water and pour it into the mold, close the lid tightly to ensure airtightness, place the mold at -80°C for 24 hours to freeze and concentrate, transfer the mold to -6°C for 72 hours to realize the self-crosslinking process of silk fibroin, and obtain multi-channel silk fibroin cryogel after demoulding.

[0053] 4) Soak multichannel silk fibroin c...

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Abstract

The invention discloses a bionic multichannel electroactive nerve conduit and a preparation method thereof. The preparation method comprises the following steps: secondary degumming of a silk thread; preparing a silk fibroin solution by taking the secondary degummed silk threads as raw materials; preparing a multi-channel silk fibroin frozen gel; preparing the multi-channel conductive frozen gel; preparing the bionic multi-channel conductive nerve scaffold; a PVDF / PLCL thin film is prepared; and fixing the bionic multi-channel conductive nerve scaffold and the PVDF / PLCL film through a surgical suture to obtain the bionic multi-channel electroactive nerve conduit. The nerve conduit has excellent biocompatibility, mechanical performance and degradation performance, the four-channel structure of the nerve conduit can limit dispersion of axons and prevent the axons from being regenerated to wrong branches, the conductive effect can stimulate maturation of Schwann cells and myelination of the axons, the piezoelectric effect can provide in-vivo electrical stimulation, the myelination process of the axons is further promoted, and the nerve conduit has a good application prospect. Therefore, the regeneration of peripheral nerves is better promoted.

Description

technical field [0001] The invention relates to the technical field of biomedical materials and regenerative medicine, in particular to a bionic multi-channel electroactive nerve guide and a preparation method thereof. Background technique [0002] According to a conservative estimate, there are more than 5 million new cases of peripheral nerve injury every year in the world. If patients cannot receive timely treatment, it is likely to lead to permanent loss of sensorimotor function. As the gold standard of clinical treatment, autologous nerve transplantation is limited by many factors such as insufficient donor sources, and an effective substitute is urgently needed. Therefore, it is of great clinical significance to develop nerve conduits that can restore the morphological continuity of damaged nerves and provide a suitable microenvironment for nerve regeneration. The nerve guides currently available on the market mainly include two forms, one is a tubular shape that can ...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/18A61L27/34A61L27/50A61L27/56A61F2/02
Inventor 曹晓东高会场马莹王浩
Owner SOUTH CHINA UNIV OF TECH
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