Application of composition in treatment of brain glioma

A brain glioma and composition technology, which is applied in the field of biomedicine to achieve the effect of high target specificity and strong tumor killing effect

Active Publication Date: 2022-05-10
BEIJING NEUROSURGICAL INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the high heterogeneity of tumors and the complexity of disease progression, chemotherapy resistance to temozolomide has become an important factor for glioma recurrence.

Method used

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  • Application of composition in treatment of brain glioma
  • Application of composition in treatment of brain glioma
  • Application of composition in treatment of brain glioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example proves that the combination of benzisoselenazole compound and temozolomide significantly inhibits the proliferation of glioma cell lines.

[0029] The benzisoselenazole compound and temozolomide described in the present disclosure were used to carry out the glioma cell proliferation inhibition experiment. Human glioma cell lines U87 and LN229 were purchased from the Cell Bank of the Chinese Academy of Medical Sciences; 20 and 295 are patient-derived glioma stem cell lines.

[0030] Cells in the logarithmic growth phase were taken and digested with trypsin to obtain a cell suspension. Count the cells, retain the amount of cells to be used, and centrifuge to obtain the cell pellet. The cells were resuspended with an appropriate amount of complete medium, LN229 and U87 cells were seeded in 96-well plates at 5000 cells / well, and 20 and 295 cells were seeded in 96-well plates at 500 cells / well. Cells in a 37 °C incubator, 5% CO 2 cultivated in the environment...

Embodiment 2

[0034] This example proves that the combination of benzisoselenazole compound and temozolomide significantly inhibits the growth of tumor in vivo.

[0035] First, a tumor-bearing mouse model was established. Female BALB / c nude mice (initial body weight 16-21g), 6-8 weeks, purchased from the Experimental Animal Center of Peking University Health Science Center, the animals were raised and operated under sterile conditions, and all animal experiments were carried out in accordance with the guidelines of international animal experiments Standard conduct. Human glioma cells LN229 were made 2×10 in PBS 7 cells / ml of cell suspension. When 100 μL of the cell suspension was injected subcutaneously into the right scapular area of ​​nude mice, the rate of tumor formation was 100%. The nude mice were divided into equal groups according to tumor size, including 6 mice in the control group (Control), 6 mice in the temozolomide group (TMZ), 5 mice in the compound single-use group, and 5 ...

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Abstract

The present disclosure relates to the use of a composition in the treatment of brain glioma. The invention provides a pharmaceutical composition with high targeting specificity for individualized diagnosis and treatment of brain glioma.

Description

technical field [0001] The present disclosure relates to the field of biomedicine, in particular, relates to the use of the composition in the treatment of glioma. Background technique [0002] Glioma is a type of intracranial tumor originating from glial stem cells or precursor cells, accounting for about 28% of all primary intracranial tumors and 80% of all primary intracranial malignant tumors. The tumor seriously damages the higher nervous system and has become the first cause of cancer death in young adults. Glioma tumors have strong heterogeneity and rapid malignant progression. Most patients need comprehensive treatments such as radiotherapy and chemotherapy after surgical resection, causing specific economic burdens on society and families. [0003] In the traditional comprehensive treatment method, temozolomide (TMZ) is used to cause DNA damage to achieve the effect of killing tumor cells, and its combined use with radiotherapy can significantly increase the median...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4188A61K31/41A61P35/00A61P25/00G16H70/40
CPCA61K31/41A61K31/4188A61P35/00A61P25/00G16H70/40A61K2300/00
Inventor 江涛曾凡常渊浩尹汉维曾慧慧
Owner BEIJING NEUROSURGICAL INST
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