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Function and application of deubiquitinating enzyme zranb1 in regulating lipid metabolism

A functional and white fat technology, applied in the fields of application, metabolic diseases, medical preparations containing active ingredients, etc., can solve the problems of undisclosed ZRANB1 protein/gene promoting fat burning, obesity and related diseases, so as to avoid insufficient effectiveness, The effect of avoiding security

Active Publication Date: 2022-06-21
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Through searching in patent databases and journal literature databases, it can be seen that ZRANB1 protein / gene belongs to the prior art, but none of the prior art discloses the related uses of ZRANB1 protein / gene to promote fat burning, treat obesity and related diseases, etc.

Method used

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  • Function and application of deubiquitinating enzyme zranb1 in regulating lipid metabolism
  • Function and application of deubiquitinating enzyme zranb1 in regulating lipid metabolism
  • Function and application of deubiquitinating enzyme zranb1 in regulating lipid metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Zranb1 Gene knockout mice have significantly reduced white adipose tissue content

[0045] Build with Loxp_Cre strategy Zranb1 In knockout mice, it was confirmed that ZRANB1 protein was completely eliminated in knockout mice by PCR identification of mouse genotype and immunoblot detection of isolated mouse embryonic fibroblasts (MEFs). Found during analysis of mouse phenotypes Zranb1 Knockout mice weighed significantly less than wild-type mice of the same age ( figure 1 ).

[0046] for confirmation Zranb1 Weight loss in knockout mice is mainly caused by reduced adipose tissue and opt for normal feeding Zranb1 The wild and gene knockout male mice were dissected and analyzed, and the brain, heart, lung, liver, kidney, and other tissues and organs were taken out of the mice for weighing and comparative analysis. The results showed that Zranb1 Compared with wild-type mice, the weights of major tissues and organs including brain, heart, lung, and kidney were n...

Embodiment 2

[0047] Example 2 Zranb1 Fat-burning gene expression is significantly increased in white fat of knockout mice

[0048] to confirm Zranb1 Expression of internal fat burning genes in white adipose tissue of knockout mice, extracted Zranb1 The white adipose tissue (scWAT and epWAT) RNA of wild-type and gene knockout mice were reverse transcribed to obtain cDNA, and then the expression of fat burning (Thermogenic genes) and adipogenesis (Adipogenic genes) related genes were detected by qPCR. The results showed that Zranb1 In the white adipose tissue of the knockout mice, the internal fat-burning gene was significantly overexpressed ( Figure 5 ),illustrate Zranb1 After the protein expression is decreased, it can promote the rapid burning of white fat in the body.

[0049] The specific implementation steps are:

[0050] 1.1 Adipose tissue RNA extraction

[0051] (1) Tissue homogenization: The adipose tissue of mice contains a large number of lipid droplets, which cannot be lys...

Embodiment 3

[0069] Example 3 H&E staining showed Zranb1 Enhanced beige in white adipose tissue in knockout mice

[0070] Both beige adipocytes and white adipocytes were differentiated from Myf5-negative progenitor cells, but their morphology and function were similar to brown adipocytes. Different from the large and stable lipid droplets in white adipocytes, beige adipocytes contain a large number of small oil droplets, the number of mitochondria in cells is significantly increased compared with white adipocytes, and they have the ability to induce UCP-1 gene expression. Therefore, beige adipocytes are also called brown-like adipocytes. to confirm Zranb1 Whether adipocyte beige is enhanced in white adipose tissue in knockout mice Zranb1 H&E was performed on the scWAT and epWAT tissues of wild-type and knockout mice respectively, and it was found that compared with wild-type mice, the Zranb1 In the adipocytes of gene knockout mice, the granular oil droplets were small and numerous, the...

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Abstract

The invention discloses the function and application of the deubiquitinating enzyme ZRANB1 in regulating lipid metabolism. Application of inhibiting ZRANB1 gene expression in preparing a product having at least one function of treating and / or preventing obesity, promoting white fat cell burning, promoting weight loss, and inhibiting subcutaneous and / or intra-abdominal white fat accumulation / accumulation. The experiment of the present invention proves that ZRANB1, as a newly discovered important fat metabolism regulation molecule, can significantly reduce the white fat content of the body after its protein level is reduced. The discovery of the function of ZRANB1 will provide important scientific basis and application value for the prevention of obesity diseases and the treatment of weight loss.

Description

technical field [0001] The invention relates to the field of biomedical care, in particular to the use of inhibiting ubiquitinase ZRANB1 gene expression in regulating lipid metabolism. Background technique [0002] Obesity is a chronic metabolic disease in which the human body's long-term energy intake exceeds consumption, resulting in excessive accumulation and abnormal distribution of white fat in the body, and reaching a health-threatening level. Obesity has become an epidemic in the modern industrial society. With the development of industrialization, the readily available high-fat food has satisfied the long-standing human desire for sugar and fat, but it has also become a burden. The three iterations of the industrial revolution have completely changed people's diet and lifestyle, resulting in a global increase in the incidence of obesity, which has become a worldwide health problem. Worldwide obesity has nearly tripled since 1975, according to data released by the Wo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61P3/04C12N9/48C12N15/57
CPCA61K45/00A61P3/04C12N9/485C12Y304/19012
Inventor 张令强李洪昌李超男崔春萍翟文静
Owner ACADEMY OF MILITARY MEDICAL SCI
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