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Reagent for rapidly recognizing glucose chirality as well as preparation method and application thereof

A glucose and chirality technology, applied in the field of analysis and detection, can solve the problems of high identification requirements and inability to recognize glucose chirality

Pending Publication Date: 2022-05-13
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a reagent for quickly identifying the chirality of glucose, so as to solve the problem that conventional reagents on the market cannot effectively and quickly identify the chirality of glucose, and the recognition requirements are relatively high

Method used

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  • Reagent for rapidly recognizing glucose chirality as well as preparation method and application thereof
  • Reagent for rapidly recognizing glucose chirality as well as preparation method and application thereof
  • Reagent for rapidly recognizing glucose chirality as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0054] Preparation of reagents for rapid recognition of glucose chirality:

[0055] S1: Mix 5.0mM 2-formylphenylboronic acid (dissolved in ethanol) and 5mM (R)-1,1,1-trifluoroisopropylamine (dissolved in ethanol) in 0.1mM triethylamine , reacted at room temperature (25° C.) for 15 minutes;

[0056] S2: Triethylamine in the solution was removed by rotary evaporation to obtain CDAsa (yield 98%);

[0057] Application of the above reagents:

[0058] A1: Derivatize the CDAsa reagent prepared in step S2 with the glucose to be tested in the buffer; set up the nuclear magnetic resonance 19 F NMR signal acquisition parameters, carry out nuclear magnetic resonance test, obtain the reagent after the derivatization reaction and the glucose of described recognition 19 F NMR signal spectrogram, denoted as the first spectrogram; 19 The sampling parameters of the F NMR spectrum are as follows: use the Bruker instrument pulse program zgig (F19CPD), the detection temperature is 298K, the sp...

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Abstract

The invention provides a reagent for rapidly recognizing glucose chirality, the reagent comprises a chiral host molecule CDAAs, the invention also provides a preparation method and application of the reagent, the method comprises the following steps: mixing a 2-formylphenylboronic acid solution, an (R)-1, 1, 1-trifluoroisopropylamine solution and a triethylamine solution for reaction, then carrying out rotary evaporation to remove triethylamine, and finally carrying out rotary evaporation to obtain the reagent for rapidly recognizing glucose chirality. According to the application, the reagent is specifically applied to glucose chirality recognition, the reagent can effectively identify the chirality of glucose, the preparation method is simple, the cost is low, and the reagent has high popularization value and commercial value.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a reagent for rapidly identifying the chirality of glucose and its preparation method and application. Background technique [0002] Chirality is a fundamental property of nature that plays a crucial role in life processes and the creation of matter. Since the importance of chirality was recognized, the demand for pure optical compounds has greatly increased in various fields, which has promoted the development of chiral detection and analysis techniques, such as chromatography, CD spectroscopy, fluorescence, nuclear magnetic resonance NMR, etc. Compared with other methods, NMR provides a facile method for a large number of compounds to obtain rich qualitative and quantitative structural information in a multi-dimensional manner at the atomic level. Therefore, this method is a powerful method in chiral recognition, determination of enantiomeric excess, determinati...

Claims

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Application Information

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IPC IPC(8): C07F5/02G01N24/08
CPCC07F5/025G01N24/087Y02P20/55
Inventor 黄碧玲黄少华
Owner NINGBO UNIV