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A kind of graphene quantum dot fluorescent probe and its synthesis method and application

A technology of graphene quantum dots and fluorescent probes, applied in fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems that threaten human health and ecological security, complex structures of aromatic compounds, and difficulty in natural degradation. Achieve great practical significance and application potential, not easy to degrade and degrade, and have good positioning effects

Active Publication Date: 2021-03-26
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The artificially synthesized aromatic hydrocarbons in environmental pollutants have complex structures and are difficult to degrade naturally. They have biological toxicity such as carcinogenicity and teratogenicity to humans, and can migrate and diffuse over long distances with media such as the atmosphere, water, soil, and organisms, causing widespread Non-point source pollution, a serious threat to human health and ecological security

Method used

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  • A kind of graphene quantum dot fluorescent probe and its synthesis method and application
  • A kind of graphene quantum dot fluorescent probe and its synthesis method and application
  • A kind of graphene quantum dot fluorescent probe and its synthesis method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: the synthesis of graphene quantum dot fluorescent probe GQDs-M

[0029] The specific synthesis process is as follows.

[0030] 1) Synthesis of graphene quantum dots GQDs:

[0031] 0.2g perylene in hot HNO 3 (80°C) and refluxed for 12h. Upon cooling to room temperature, the mixture was diluted with deionized water and the acid was removed using a 0.22 μm Millipore filter. The obtained brownish yellow crude product was dispersed in 1.5M hydrazine hydrate and sonicated for 2h. The suspension was transferred to a hydrothermal reactor at 200°C for 10 h. After cooling to room temperature, insoluble carbon products were removed with a 0.22 μm microporous membrane. Then, it was dialyzed with a dialysis bag (3500Da) for about one week to obtain graphene quantum dots (graphene quantum dots, GQDs) as shown in formula II.

[0032] 2) Synthesis of graphene quantum dots GQDs-M:

[0033]0.1 mmol of methyl red was dissolved in 15 mL of water / DMSO mixture (1:2), 76....

Embodiment 2

[0034] Embodiment 2: the spectrum of graphene quantum dot probe:

[0035] Graphene quantum dots GQDs and graphene quantum dots GQDs-M were dispersed in aqueous solution, and their infrared spectra, Raman spectra, absorption spectra and fluorescence spectra were tested. The infrared spectrum test uses a Fourier transform infrared spectrometer (Bruker / Tensor II), with a test range of 4000-400cm -1 . The Raman spectrum test uses a microscopic Raman spectrometer (LabRAM Aramis), with a test range of 4000-100cm -1 . The ultraviolet spectrum test uses an ultraviolet-visible spectrometer (SHIMADZU / UV-2600), and the test range is 400-800nm. The fluorescence spectrum test uses a fluorescence spectrophotometer (Perkin Elmer / LS-45), the excitation wavelength range is 200-600nm, and the emission wavelength range is 400-800nm.

[0036] figure 1 , figure 2 It is the spectral analysis of the graphene quantum dot probe of the present invention. figure 1 A is the infrared spectrum of g...

Embodiment 3

[0037] Embodiment 3: the morphology structure of graphene quantum dot probe:

[0038] Firstly, the graphene quantum dot probe suspension was filtered through a 0.22 μm microporous membrane, and then dialyzed with a dialysis membrane with a molecular weight cut-off of 3500 Da for about one week. Particle characterization was performed using Transmission Electron Microscope (TEM).

[0039] image 3 A is the TEM characterization of the graphene quantum dot probe of the present invention. It can be seen that the nanoparticles are well dispersed, in a regular spherical shape, with an average particle size of 2±1nm. image 3 B is the TEM image of graphene quantum dot probes co-cultured with S12 for 12 hours. It can be seen that the graphene quantum dot probes are evenly distributed in the bacteria and its surrounding environment.

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Abstract

The invention discloses a graphene quantum dot fluorescent probe. The structure of the graphene quantum dot fluorescent probe is shown in formula I in the description. According to the graphene quantum dot fluorescent probe, an azo dye is taken as a target compound, an azo dye decoloration bacterial strain is taken as an experimental object, firstly, a graphene quantum dot fluorescent probe technology is applied to the visualization of tracer microbial degradation, and the chromogenic process of the degradation of the azo dye in bacterial cells are traced through fluorescent labeling to elucidate the characteristics of capture and degradation transfer of azo dye molecules by microbial bacterial cells. The development of the study establishes a bran-new microbial tracer study mode based ona chemical fluorescent labeling technology, so that fast and high-sensitivity analysis and identification are carried out on a single cell more vividly, visually, efficiently and specially, a novel tool can be provided for efficient tracing of functional microbes, the technical support can also be provided for single cell research of the functional microbes and in-depth exploration of uncultured microbes, and practical significance and application potential for the prevention and control of toxic aromatic pollutants are great.

Description

Technical field: [0001] The invention relates to the technical field of functional microorganism tracing, in particular to a graphene quantum dot fluorescent probe and its synthesis method and application. Background technique: [0002] At present, with the continuous development of the economy and the progress of the society, the problem of environmental pollution in our daily production and life has become very serious. Environmental pollution will not only cause serious damage to the economy and ecology, but also seriously threaten people's life, health and safety. The artificially synthesized aromatic hydrocarbons in environmental pollutants have complex structures and are difficult to degrade naturally. They have biological toxicity such as carcinogenicity and teratogenicity to humans, and can migrate and diffuse over long distances with media such as the atmosphere, water, soil, and organisms, causing widespread The non-point source pollution seriously threatens human...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64C07D493/08C09K11/06
Inventor 许玫英刘飞廖兵罗业燊孙国萍
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY