Microbial remediation method applied to non-soil dressing mining area

A technology of microbial remediation and microbial domestication, applied in the field of environmental remediation, can solve problems such as difficult growth, heavy metal leaching, and difficult application, and achieve sulfide stability, remediation effect, sustainable effect, and low remediation cost

Inactive Publication Date: 2022-05-13
青海绿道环保生物科技有限公司
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  • Abstract
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Problems solved by technology

Traditional chemical curing restoration techniques (such as figure 2 ) mainly uses chemical agents to react with heavy metal elements so that they are no longer leached by rainwater or absorbed by plant roots, but this cannot avoid the leaching of acid, iron and heavy metals mediated by autotrophic acidophilic microorganisms, so it is difficult to apply to heavy metals Continuously leaching polluted sites
In addition, the traditional vegetation restoration te

Method used

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  • Microbial remediation method applied to non-soil dressing mining area
  • Microbial remediation method applied to non-soil dressing mining area
  • Microbial remediation method applied to non-soil dressing mining area

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Embodiment Construction

[0037] The present invention will be further described below in conjunction with accompanying drawing by specific embodiment:

[0038] The microbial remediation method applied to the guest-free soil mining area is carried out according to the following steps,

[0039] (1) Soil sampling in the area to be restored;

[0040] (2) Microbial domestication and isolation:

[0041] 1) Inoculate the indigenous mixed bacteria in the slag sample to a Cr(VI) concentration of 100 mg L at an inoculation amount of 10%. -1 In the enrichment medium of , cultured at 30°C under aerobic and anaerobic conditions for 7 days;

[0042] 2) After 7 days, transfer the obtained bacterial solution as mother solution to a solution containing Cr(VI) concentration of 200 mg L -1 In the NB culture medium, they were cultured at 30°C under aerobic and anaerobic conditions for 7 days, and the reduction rate of Cr(VI) and the number of bacteria in the culture medium were regularly detected during the acclimatio...

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Abstract

The invention discloses a microbial remediation method applied to a non-soil dressing mining area, which mainly utilizes the organic combination of various strains such as sulfate reducing bacteria, photosynthetic bacteria, nitrogen-fixing bacteria and the like to construct a healthy underlying microbial ecosystem on the surfaces of bare rocks and sandy soil, and the underlying microbial ecosystem can be self-balanced and self-grown under the illumination condition. By secreting extracellular polymeric substances to be adhered to the surfaces of bare rock and sandy soil to grow, water contained in the air can be efficiently absorbed to be supplied to microorganisms, and various repairing functions such as water retention, ecological environment improvement and plant root system development promotion are achieved. Sulfide generated by remediation is stable, low in solubility and environment-friendly and has a buffering effect, after a bottom layer microbial ecological system is constructed, heavy metal is fixed in the farmland in a sulfide mineral form, crops can efficiently and rapidly grow in the farmland polluted by the heavy metal, and qualified agricultural products are produced. And the used microorganisms are environment-friendly microorganisms, so that the environmental ecology can be fundamentally improved, and the durability is realized.

Description

technical field [0001] The invention relates to the technical field of environmental restoration, in particular to a method for regreening restoration of gangue slag microorganisms in non-guest soil mining areas. Background technique [0002] There are many kinds of mineral resources in Qinghai and other western regions of my country, such as Qilian Mountain coal-bearing belt, but there is serious chromium pollution. Chromium slag is a heavy metal hazardous waste, and the hexavalent chromium (Cr 6+ ) is soluble and unstable, has strong oxidative toxicity, and can cause damage to the human body and crops. Studies have shown that calcium chromate (hexavalent chromium) contained in chromium slag also has strong carcinogenic and mutagenic properties. Chromium slag without harmless treatment will seriously pollute surface water, groundwater and soil, and pose a huge threat to the ecological environment and the safety of people's lives and properties. For this reason, many plac...

Claims

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Application Information

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IPC IPC(8): C12N1/36C12N1/20C09K17/14B09C1/10C09K101/00
CPCC12N1/36C12N1/20C09K17/14B09C1/10C09K2101/00B09C2101/00
Inventor 张春
Owner 青海绿道环保生物科技有限公司
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