Microbial remediation method applied to non-soil dressing mining area
A technology of microbial remediation and microbial domestication, applied in the field of environmental remediation, can solve problems such as difficult growth, heavy metal leaching, and difficult application, and achieve sulfide stability, remediation effect, sustainable effect, and low remediation cost
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[0037] The present invention will be further described below in conjunction with accompanying drawing by specific embodiment:
[0038] The microbial remediation method applied to the guest-free soil mining area is carried out according to the following steps,
[0039] (1) Soil sampling in the area to be restored;
[0040] (2) Microbial domestication and isolation:
[0041] 1) Inoculate the indigenous mixed bacteria in the slag sample to a Cr(VI) concentration of 100 mg L at an inoculation amount of 10%. -1 In the enrichment medium of , cultured at 30°C under aerobic and anaerobic conditions for 7 days;
[0042] 2) After 7 days, transfer the obtained bacterial solution as mother solution to a solution containing Cr(VI) concentration of 200 mg L -1 In the NB culture medium, they were cultured at 30°C under aerobic and anaerobic conditions for 7 days, and the reduction rate of Cr(VI) and the number of bacteria in the culture medium were regularly detected during the acclimatio...
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