Rapid detection kit for heavy metal Al < 3 + > and application thereof

A detection method and mixed solvent technology are applied in the field of rapid detection kits for metal Al3+, which can solve the problems of low enzyme-like activity and restrict the development of nanozymes, and achieve the effect of easy operation.

Inactive Publication Date: 2022-05-27
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In addition, with the development of nanotechnology, nanomaterials that mimic the activity of natural enzymes are named nanozymes, which exhibit many advantages such as wider acid-base range, temperature tolerance range, lower production cost, and higher stability than natural enzymes. But at the same time, its enzyme-like activity is generally lower than that of natural enzymes, which seriously restricts the development of nanozymes.

Method used

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  • Rapid detection kit for heavy metal Al &lt; 3 + &gt; and application thereof
  • Rapid detection kit for heavy metal Al &lt; 3 + &gt; and application thereof
  • Rapid detection kit for heavy metal Al &lt; 3 + &gt; and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Synthesis of single-atom cerium nanozyme

[0065] Cetyltrimethylammonium bromide (CTAB), ammonium persulfate (APS), cerium nitrate, dimethyl sulfoxide (DMSO), lithium chloride were provided by Shanghai Aladdin Biochemical Technology Co., Ltd.; polypyrrole (PPy ) and 3,3',5,5'-tetramethylbenzidine (TMB) were provided by Shanghai McLean Biochemical Technology Co., Ltd.

[0066] 1. Synthesis of single-atom cerium nanomaterials

[0067] Single-atom cerium nanomaterials were synthesized by dissolving 0.73 g of cetyltrimethylammonium bromide (CTAB) in 60 mL of 1 M HCl solution by sonication under an ice bath. Then 1.37 g of ammonium persulfate (APS) was added to the above solution and stirred evenly to obtain a CTBA template with white precipitate. An additional 1.0 mL of pyrrole was added and stirring was continued for one day to achieve polymerization. The black precipitate was collected by vacuum filtration and washed with water and ethanol. The mixture was t...

Embodiment 2

[0070] Example 2 Application of Al based on single-atom cerium nanozyme-like phosphatase activity 3+ Establishment of detection methods

[0071] 1. Experimental materials

[0072] 5% Nafion was provided by Shanghai Biotechnology Co., Ltd.; Tris-HCl buffer (pH 9.0) and absolute ethanol (C 2 H 5 OH) prepared by Shanghai Yuanye Biotechnology Co., Ltd.; 4-Nitrophenylphosphate disodium salt (p-NPP), EDTA-2Na, commercial alkaline phosphatase (ALP), FCP962 The 96-well plate was purchased from Shanghai Biyuntian Biotechnology Co., Ltd., and 4-methylumbelliferone disodium phosphate (4-MUP) was purchased from Xi'an Qiyue Biotechnology Co., Ltd.; Fe 3+ , Cu 2+ , Cd 2 + , Hg 2+ , K + , Na + , Ca 2+ , Mg 2+ , Zn 2+ , Pb 2+ , Cr 6+ , As 5+ , F - , Cl - , Br - , NO 3 - , SO 4 2- , PO 4 3- , Cr 3+ , Al 3+ The standard solution standard is provided by Qingdao Quanchang Technology and Trade Co., Ltd.;

[0073] Vermicelli, vermicelli, wide noodles, peach cake, red bea...

Embodiment 3

[0083] Example 3 Detection condition optimization results

[0084] 1. Optimization of single-atom cerium nanozyme phosphatase activity

[0085] In order to maximize its phosphatase-like activity, its possible influencing factors were optimized and compared with commercial alkaline phosphatase, such as reaction pH ( Figure 4A), the reaction temperature ( Figure 4 B), the reaction time ( Figure 4 C), and storage time ( Figure 4 D). Experiments proved that the optimal reaction conditions were: Tris-HCl buffer pH 9.0, reaction temperature 37 °C, reaction time 15 min and storage time 30 d.

[0086] 2. Fitting parameters of single-atom cerium nanozyme-like phosphatase activity

[0087] The magnitude of single-atom cerium nanozyme-like phosphatase activity was evaluated under optimal reaction conditions. By calculating enzyme kinetic parameters v max , K m ( Figure 5 A) and its enzyme activity SA ( Figure 5 B) to evaluate the magnitude of single-atom cerium nanozyme...

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Abstract

The invention provides a rapid detection kit for heavy metal Al < 3 + > and an application method of the rapid detection kit for heavy metal Al < 3 + >. The application method comprises the following steps: (1) verifying and evaluating the activity of monatomic cerium (Single atom Ce-N-C, SACe-N-C) nano-enzyme phosphatase (PPA-like), and (2) constructing a detection system of the rapid detection kit. According to the method, Al < 3 + > can react with oxygen atoms in a monatomic cerium nano-enzyme structure to form an Al-O bond, so that the activity of monatomic cerium nano-enzyme phosphatase is remarkably reduced, and rapid and efficient detection of Al < 3 + > in a system can be realized within 4 minutes.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a kind of anti-metal Al 3+ The rapid detection kit and its application. Background technique [0002] Al 3+ It is one of the most common metals used today. In daily life, Al 3+ It is often used as food additives such as curing agent, leavening agent, stabilizer, anti-caking agent and coloring agent in the food production process. For example, in fried food, in soy products Al 3+ Exceeding the standard is very significant. Excessive intake of Al 3+ It will cause certain harm to the human body and cause Alzheimer's disease and Parkinson's disease [1, 2]. However, the current detection of Al 3+ The main methods are atomic absorption spectrometry [3-5], inductively coupled plasma atomic emission spectrometry [6, 7] and so on. However, these methods all have certain limitations, such as the need for expensive equipment and professional operators, which seriously ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/10B01J27/24B01J35/00B01J35/12G01N21/78
CPCB01J23/10B01J27/24B01J35/0013B01J35/0046B01J35/12G01N21/78G01N2021/775
Inventor 程楠宋光春黄昆仑罗云波许文涛贺晓云庞晓旭刘清亮
Owner CHINA AGRI UNIV
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