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Universal preclinical biological distribution detection kit for NK (Natural Killer) cell treatment products

A technology of NK cell and distribution detection, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems that are not suitable for the construction of qPCR

Active Publication Date: 2022-06-03
SHANGHAI MEDICILON INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In addition, for CAR-NK or other modified NK cell therapy products that are directly coupled to the CAR part outside the cell, it is not suitable for the method of constructing qPCR based on the CAR sequence
For example, for directly coupled CAR, the CAR itself has been expressed as a protein product for coupling, so the CAR-based detection method at the DNA level is no longer applicable

Method used

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  • Universal preclinical biological distribution detection kit for NK (Natural Killer) cell treatment products
  • Universal preclinical biological distribution detection kit for NK (Natural Killer) cell treatment products
  • Universal preclinical biological distribution detection kit for NK (Natural Killer) cell treatment products

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Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

[0050] Table 1

Embodiment 3

Example 2 KLRC1, KLRC3, KLRK1, and KLRK3, the 4 pairs of primers corresponding to the four gene sequences can be potentially applied to distinguish between human NK cells and non -human animal genes, that is, specific expansion on human NK cells has specific expansion on human NK cells Increasing the value -added value in the mouse -based DNA. This embodiment further sequencies based on sequencers. The sequencing results are shown in Table 2.

[0052] Table 2

The gene sequence of KLRC1-1F is shown in SEQ ID No.2. The gene sequence of KLRC1-1R is shown in SEQ ID No.3. The gene sequence of KLRC1-3F is shown in SEQ ID No.5. The gene sequence of KLRC1-3R is shown in SEQ ID No.6. The gene sequence of KLRK1-1F is shown in SEQ ID No.7. The gene sequence of KLRK1-1R is shown in SEQ ID No.8. The gene sequence of KLRK1-3F is shown in SEQ ID No.9. The gene sequence of KLRK1-3R is shown in SEQ ID No. 10.

[0053] Table 2 proves that KLRK1 (human killing cell condenser sampled vitamine fami...

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Abstract

The invention discloses a universal preclinical biological distribution detection kit for NK cell treatment products. The invention also relates to a specific application of the gene sequence as shown in SEQ ID No.1 in distinguishing human NK cells and non-human animal genes. By proposing a specific DNA sequence, a DNA sequence of a human NK cell source and a DNA sequence of a non-human animal gene source can be specifically distinguished, a qPCR system for distinguishing human NK cell genes and non-human animal genes is constructed based on the specific DNA sequence, and a primer pair and a probe group are provided for NK cells and derived cell treatment products thereof. And the universal preclinical biological distribution detection kit for the NK cell treatment products is successfully designed, so that convenience is provided for preclinical research of NK cells and derived cell treatment products thereof.

Description

[0001] NK cell therapy products General -purpose pre -clinical biological distribution test kit. Technical field [0002] The present invention involves biological distribution analysis, pharmacokinetics, poison dynamics, cell therapy drug safety evaluation and preclinical research fields, especially a common clinical biological distribution detection kit of NK cell therapy products. Background technique [0003] Nature Killer Cell (NK cells) is derived from bone marrow lymph -like stem cells. Its differentiation and development depend on bone marrow and gland micro -environment. It is mainly distributed in bone marrow, peripheral blood, liver, spleen, lungs, and lymph nodes. NK cells are different from T cells and B cells. It is a type of lymphocytes that can kill tumor cells and virus infection cells without pre -allergens. In recent years, with the continuous development of cell gene therapy, T cells have been developed by researchers in the field of biomedicine into Chimeric A...

Claims

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/6888C12N15/11
CPCC12Q1/6851C12Q1/6888C12Q2600/166C12Q2531/113C12Q2545/114C12Q2527/107C12Q2561/101C12Q2563/107Y02A50/30
Inventor 章登吉袁雨琼白振振梁冉冉陈春麟朱焕章
Owner SHANGHAI MEDICILON INC