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Skin care product raw material, cosmetic, preparation method and application

A technology for skin care products and cosmetics, which is used in cosmetic preparations, cosmetics, preparations for skin care, etc.

Active Publication Date: 2022-06-10
上海龙殷生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The production and preparation of α-glycerol glucoside (α-GG) by the existing biotransformation method usually uses sucrose as the glycosyl donor, and a large amount of fructose will be continuously produced during the reaction process By-products, since the polarity of polyhydroxy glycerol glycosides and polyhydroxy fructose is relatively close, the higher the fructose content, the higher the separation cost between the two

Method used

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  • Skin care product raw material, cosmetic, preparation method and application
  • Skin care product raw material, cosmetic, preparation method and application
  • Skin care product raw material, cosmetic, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 The acquisition and expression of the difference between the difference between sucrose phosphoric acid enzymes and amoone sugar is different.

[0046] The sucrose phosphorase (LPP) used in this embodiment is derived from Leuconostocpseudomesenteroides. Its gene sequence is listed in sequence 1.Biological, the goods number VT1212), builds the LLP enzyme expression plasmid PET-30A-LPP.

[0047] The practical amoone sugar in this embodiment (NTDAE) gene comes from Novibacillusthermophilus. Its gene sequences are listed in sequences.In (+), the expression plasmid PET-30A-NTDAE is built into NTDAE enzyme.

[0048] In this embodiment, the expression plasmid PET-30A-LPP and expression plasmid PET-30A-NTDAE entrusted Kim Weizhi Biotechnology Co., Ltd. to complete genetic synthesis and complete the construction of Atacon, preparation and purification.Before the use of the plasmid DNA, according to the instructions marked on the plasmid product description of Jin Weizhi Bi...

Embodiment 2

[0055] Example 2 enzyme catalytic process (control group).

[0056] PET-30A-LPP obtained by embodiments 1 expresses the reorganization bacteria as LPP crude enzymes.

[0057] The production response process of glycoside glycoside refers to the biological process of glycerin glycoside in existing technologies.

[0058] The reaction process is as follows:

[0059] According to the formula of the glycelasses glucoside enzyme enzyme enzymes listed in the table.10 LPP crude enzymes, 50 parts of glycerin, 50 parts of water and 50 parts of sucrose. After continuing to react 24h, using 50 % sulfuric acid will react systemic acid to PH3.0 to terminate the reaction. The reaction solution can be used for the separation and testing of subsequent glycoglycoside.

[0060] Table .1 Glyceride glucosidin enzymatic reaction formula table

[0061]

[0062]

[0063] After the reaction solution is appropriately diluted, the HPLC-ELSD detection is appropriately diluted.EssenceAs a result figure 1 A...

Embodiment 3

[0064] Example 3 enzyme catalytic process (dual enzyme process).

[0065] PET-30A-LPP obtained by embodiments 1 according to its steps to express the reorganization bacteria and PET-30A-NTDAE expression restructuring bacteria as LPP crude enzymes and NTDAE crude enzymes, respectively.

[0066] The production response process of glycin glycoside is referred to the technical solution of the present invention. The reaction process is as follows:

[0067] According to the formula of the glycelaceladidinase enzyme enzymes listed in the table.Glycerin, 50 parts of water and 50 parts of sucrose, continue to react for 24h.

[0068] After that, further throw 30 NTADE crude enzymes in the reaction system, 118 borax, and then adjust the temperature of the reaction tank to 55 ° C, further stir the reaction of 60h, and then use 50 % sulfuric acid to terminate the reaction system to pH3.0 to terminate the reaction.Can be used for the separation and testing of subsequent glycoglycoside.

[0069]...

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Abstract

The invention discloses a skin care product raw material, a cosmetic, a preparation method and an application, and provides a skin care product raw material containing glycerol glucoside and psicose. The alpha-glycerol glucoside and the fructose are obtained by catalyzing glycosyl transfer reaction through sucrose phosphate synthetase, and then isomerization reaction of the fructose is catalyzed by further adding D-psicose 3-epimerase, so that the fructose in the product further forms psicose. According to the method disclosed by the invention, the production cost is greatly reduced, and when the psicose and the glycerol glucoside are cooperatively used, the psicose and the glycerol glucoside have a synergistic promoting effect on reducing transdermal moisture loss and improving the water holding capacity of the skin, and the effect is higher than the effect of superposition of independent effects of the psicose and the glycerol glucoside.

Description

Technical field [0001] The present invention involves a skin care product raw material, which is specifically for skin care products, preparation methods and applications with α-glycelaside glucoside as the main component. Background technique [0002] The industry recognizes that glycerin glycosides have excellent moisturizing capabilities, especially the α-glycerol glucoside (α-Gg) composed of glucoside composed of glycerin and glucoside bonds has the effects of moisturizing, antioxidant and anti-aging. At the same time, the substance has lowThe characteristics of calories, probe yuan characteristics, and protein stability function.The molecular weight of α-glycelaglycoside (α-Gg) is small and is easily absorbed by the skin.It not only has the effect of moisturizing, but also enhances the ability of the skin to adapt to bad weather, such as anti -drying and freezing ability; it can also remove free radicals produced by α and β -rays, effectively protecting the skin from radiati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/60A61Q19/00C12P19/44C12P19/24C12P19/02
CPCA61K8/60A61K8/602A61Q19/00C12P19/44C12P19/24C12P19/02
Inventor 张鹏强耀锋樊冰陈志超李庆廷
Owner 上海龙殷生物科技有限公司
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