Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture method for efficiently propagating arbuscular mycorrhizal fungal spores through matrix nutrient layering

A technique for arbuscular mycorrhizal fungi and a culture method is applied in the field of matrix nutrient layered culture to achieve the effects of reducing costs, simplifying tray attachments, and improving adsorption and fertilizer retention performance.

Pending Publication Date: 2022-06-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem to be solved in the present invention is that in the process of realizing the efficient reproduction of AM fungi through symbiotic cultivation between AM fungi and host plants, the root system of host plants can form an efficient symbiotic infection system with AM fungi only under low-phosphorus culture conditions. In the subsequent cultivation process, if the low phosphorus level is maintained, the photosynthetic efficiency of the host plant will be affected, resulting in the limitation of the amount of photosynthetic products exported to the AM fungi, which will eventually lead to a low reproduction number of the AM fungi; During the process, direct topdressing of high-concentration phosphorus fertilizer will cause the host root system to directly absorb phosphorus fertilizer, reduce the permeability of the host plant cell membrane, reduce the output of photosynthetic products to AM fungi, lead to the collapse of the symbiotic system, and eventually reduce the reproduction of AM fungi The number of this training puzzle

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture method for efficiently propagating arbuscular mycorrhizal fungal spores through matrix nutrient layering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0045] 1. Layered filling of culture substrate:

[0046] 1. Selection and pretreatment of culture substrates:

[0047] The culture substrate is a mixture of zeolite and river sand, the zeolite passes through a 2.5mm sieve, and the river sand is ordinary river sand that passes through a 2.0mm sieve. Mix river sand and zeolite in a ratio of 1:1 by volume, and divide the mixed matrix into two parts for use: one part of the mixed medium does not add any nutrients, which is called a phosphorus-free mixed culture medium; the other part of the mixed medium is mixed per liter. 200ml of Hoagland's nutrient solution with a concentration of 3 times was applied to the substrate, which was called a high-phosphorus mixed culture medium.

[0048] 2. The choice of culture matrix isolation nylon mesh:

[0049] A nylon mesh with a pore size of 400 meshes (corresponding to a pore size of 38 μm) was used to separate the upper and lower partitions of the culture substrate in the culture vessel. ...

Embodiment 2

[0066] 1. Layered filling of culture substrate:

[0067] 1. Selection and pretreatment of culture substrates:

[0068] The culture medium is a mixture of zeolite and river sand, the zeolite is passed through a 2.0mm sieve, and the river sand is ordinary river sand passed through a 2mm sieve. Mix river sand and zeolite in a ratio of 1:1 by volume, and divide the mixed matrix into two parts for use: one part of the mixed medium does not add any nutrients, which is called a phosphorus-free mixed culture medium; the other part of the mixed medium is mixed per liter. 200ml of Hoagland nutrient solution with a concentration of 2.5 times was applied to the substrate, which was called a high-phosphorus mixed culture medium.

[0069] 2. The choice of culture matrix isolation nylon mesh:

[0070] A nylon mesh with a pore size of 400 meshes (corresponding to a pore size of 38 μm) was used to separate the upper and lower partitions of the culture substrate in the culture vessel.

[007...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a culture method for efficiently propagating arbuscular mycorrhizal fungal spores by layering matrix nutrients. The culture method comprises the following steps: (1) filling a high-phosphorus mixed matrix at the lower layer: filling a high-phosphorus mixed culture matrix at the bottom of a culture container; (2) filling a middle-layer non-phosphorus mixed medium: uniformly filling the surface of the paved high-phosphorus mixed medium with a non-phosphorus mixed medium; 3) laying an isolation nylon net and filling an upper-layer non-phosphorus mixed medium: laying the isolation nylon net on the middle-layer non-phosphorus mixed culture medium, and filling the isolation nylon net with the non-phosphorus mixed culture medium; 4) sowing the germinated host plant seeds and AM fungus strains in a non-phosphorus mixed matrix for culturing; and 5) after culture is completed, cutting off the overground part of the host plant, carrying out post-treatment on the culture medium, and collecting AM fungal spores in the culture medium under the nylon net.

Description

technical field [0001] The patent of the invention belongs to the research field of arbuscular mycorrhizal fungi, and specifically relates to a stratified culture method of matrix nutrients for improving the spore reproduction efficiency of arbuscular mycorrhizal fungi in the process of symbiotic culture. Background technique [0002] Arbuscular mycorrhizal AM fungi are a class of obligate living symbiotic fungi. So far, they cannot be cultured purely in vitro, and can only rely on symbiosis with living host plants to complete their reproduction process. In the symbiotic system formed by AM fungi and plants, AM fungi supply 50%-70% of the total mineral nutrient absorption of the symbiotic plants, while the symbiotic plants feed back 4%-17% of the photosynthetic products of the AM fungi themselves. Material exchange is the basis for the establishment of a symbiotic system. [0003] At present, the cultivation of AM fungi is mostly carried out by pot culture method under low ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12M1/00C12R1/645
CPCC12N1/14C12N3/00C12M25/14
Inventor 邢礼军张淑彬王幼珊李亚星刘建斌武凤霞
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com