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Application of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer

A technology for esophageal cancer and uses, applied in the field of esophageal cancer auxiliary diagnosis and targeted therapy by KRT15

Pending Publication Date: 2022-06-28
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Based on the fact that there are no reports on the mechanism of action of KRT15 in esophageal cancer cells

Method used

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  • Application of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer
  • Application of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer
  • Application of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Expression of KRT15 in patients with esophageal cancer tissue

[0064] We used RNA in situ hybridization to analyze the expression of KRT15 in esophageal cancer tissues and its relationship with the prognosis of patients. 1013 postoperative tissue samples from esophageal cancer patients (without any preoperative treatment) collected in Linzhou Esophageal Cancer Hospital, Henan Province from 2011 to 2015 were selected for RNA in situ hybridization analysis. See the Materials and Methods section below for specific steps.

[0065] The results showed that the expression of KRT15 differed greatly among different esophageal cancer patients. It was highly expressed in 43.93% (445 / 1013) of esophageal cancer tissues (mean intensity × area score was above 3), 38.60% (391 / 1013) ) was not expressed in esophageal cancer tissues (the mean score of intensity × area was 0) ( figure 1 ). The correlation analysis of clinicopathological parameters showed that KRT15 expression...

Embodiment 2

[0069] Example 2 The effect of KRT15 on the proliferation of esophageal cancer cells

[0070] To explore the mechanism by which KRT15 promotes the development of esophageal cancer, we examined the effect of KRT15 on the malignant phenotype of esophageal cancer cells. The esophageal cancer cell lines KYSE150, KYSE450, and KYSE510 with high KRT15 expression were transiently transfected with two siRNAs specific for KRT15, ​​respectively. The controls included parental group or transfection non-silence group. When the cells were completely adherent, the cell viability was detected by the CCK-8 method. The results showed that after knockdown of KRT15, ​​the proliferation ability of esophageal cancer cells was significantly lower than that of the control group ( Figure 5 ).

Embodiment 3

[0071] Example 3 The effect of KRT15 on the distribution of esophageal cancer cell cycle

[0072] Flow cytometry was used to analyze the ratio of G0 / G1, G2 and S phase cells in the cell cycle, and the results showed that knockdown of KRT15 promotes G2 / M phase arrest in esophageal cancer cells ( 6A to 6B ).

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Abstract

The invention relates to application of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer. Specifically, the invention relates to application of a detection agent for detecting the expression level of KRT15 from a subject sample in preparation of a reagent for auxiliary diagnosis and / or prognosis of esophageal cancer. On the other hand, the invention relates to application of a KRT15 targeting reagent in preparation of a medicine for treating esophageal cancer, and the KRT15 targeting reagent is a reagent for inhibiting and / or reducing the expression level of a KRT15 gene and / or protein or antagonizing the function of the KRT15 protein.

Description

technical field [0001] The present invention relates to the field of diagnosis and treatment of esophageal cancer. Specifically, the present invention relates to the use of KRT15 in auxiliary diagnosis and targeted therapy of esophageal cancer. Background technique [0002] KRT15 (Keratin 15, CK15 / K15) belongs to the keratin family and is an intermediate fibrillar protein responsible for maintaining the structural integrity of epithelial cells (Omary et al., 2004). A variety of keratins have been found, which can be divided into type I keratin and type II keratin according to their isoelectric points and sequences: Type I keratin is an acidic protein with a molecular weight of 40-55KDa, including K9-K28, K31 -K40; Type II keratin is a neutral or basic protein with a molecular weight of 56-70KDa, including K1-K8, K71-K86. Usually two types of keratin are expressed in pairs, and the two obligately and non-covalently associate as heteromers or type I / II complexes, which aggre...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574A61K45/00A61K31/7088A61P35/00
CPCC12Q1/6886G01N33/57484G01N33/57407A61K45/00A61K31/7088A61P35/00C12Q2600/118C12Q2600/136C12Q2600/158
Inventor 王明荣郝佳洁杨荔艳蔡岩徐昕张钰张娜苑青孙晓男
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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