Lactobacillus paracasei for preventing infant streptococcus infection and application of lactobacillus paracasei

A technology of Lactobacillus and paracheese, applied in medical preparations containing active ingredients, bacteria, antibacterial drugs, etc., can solve the problem of increasing the incidence of necrotizing enteritis, liver and kidney damage and hearing loss in children, and increasing the incidence of hospitalized children. case fatality rate

Pending Publication Date: 2022-07-08
山东百沃生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment of streptococcal infection in infants and young children mainly focus on perinatal care,

Method used

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  • Lactobacillus paracasei for preventing infant streptococcus infection and application of lactobacillus paracasei
  • Lactobacillus paracasei for preventing infant streptococcus infection and application of lactobacillus paracasei
  • Lactobacillus paracasei for preventing infant streptococcus infection and application of lactobacillus paracasei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Screening of Lactobacillus paracasei VHProbi O44

[0033] 1. Bacteria isolation

[0034] Prepare MRS (Man Rogosa Sharpe) broth: pure water 1L, peptone 10g, beef extract 10g, yeast extract 5.0g, sodium acetate 5g, glucose 5g, potassium dihydrogen phosphate 2g, Tween 80 1.0mL, citric acid Diamine 2.0g, calcium carbonate 20g, magnesium sulfate heptahydrate 0.58g, manganese sulfate heptahydrate 0.25g, adjusted pH 6.2-6.5.

[0035] Configure MRS agar medium: add 15g agar to 1LMRS broth.

[0036]According to the 2019 version of the "Human Genetic Resource Bank Ethical Specifications", after signing the project commitment letter and informed consent with the sample provider, according to the standard operating specifications of the biobank, take 1g of fresh fecal samples from healthy infants who have not eaten probiotic preparations within half a year. After being diluted with sterile saline, put it into a sterile sample bag, beat and mix with a homogenizer; take 1...

Embodiment 2

[0046] Embodiment 2, O44 strain identification

[0047] 1. Identification of colony morphology

[0048] The O44 strain was inoculated on the MRS agar medium, and after anaerobic culture at 37°C for 24 hours, the single colony of O44 was milky white, the diameter of the colony was more than 1.0mm, and the surface was smooth. Single or paired, arranged in chains.

[0049] 2. Identification of physiological and biochemical characteristics

[0050] The preparation of the inoculum in this example is as follows: under aseptic conditions, take an appropriate amount of fresh O44 bacterial solution, centrifuge at 5000 rpm / min for 5 min, wash twice with PBS buffer, and then resuspend the bacteria with the same volume of PBS buffer and dilute 50 times as the inoculum.

[0051] 2.1. Salinity tolerance test

[0052] Under sterile conditions, add 190 μL of BSM liquid medium with a salt concentration of 1%, 2%, 3%, 4%, 5%, 6%, 7%, and 8% to a 96-well plate, respectively, for each salt co...

Embodiment 3

[0097] Example 3 Tolerance test of Lactobacillus paracasei VHProbi O44 to artificial gastric juice and artificial intestinal juice

[0098] 1. Preparation of artificial gastric juice

[0099] Weigh 5 g of peptone, 2.5 g of yeast extract, 1 g of glucose and 2 g of NaCl, add 1000 mL of distilled water, adjust pH to 3.0 with dilute hydrochloric acid, and then sterilize at 115 °C for 20 min. Then add 3.2 g of porcine mucosal pepsin before use, shake well to dissolve, and place in a warm water bath for 1 hour in a 37°C water bath shaker to simulate human body temperature.

[0100] 2. Preparation of artificial intestinal juice

[0101] Weigh 5 g of peptone, 2.5 g of yeast extract, 1 g of glucose, KH 2 PO 4 6.8g and 3.0g of bovine bile salt, add 77mL of 0.2mol / L NaOH solution, dilute to 1000mL, adjust pH to 6.8±0.1 with dilute hydrochloric acid or sodium hydroxide solution, and sterilize at 115℃ for 20min. Then add 1g of pancreatin before use, shake well to dissolve, and put it ...

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Abstract

The invention belongs to the technical field of screening and application of probiotics, and particularly relates to a Lactobacillus paracasei strain for preventing streptococcus infection of infants and an application of the Lactobacillus paracasei strain. The lactobacillus paracasei has a very strong antibacterial effect on beta-hemolytic streptococcus and is preserved in the China Center for Type Culture Collection on May 24, 2021, and the preservation number of the lactobacillus paracasei is CCTCC (China Center for Type Culture Collection) NO: M2021591. The strain can effectively prevent streptococcus infection, especially streptococcus infection of infants, can significantly reduce the level of inflammatory factors in the body, alleviate the degree of shedding of intestinal mucosa layers, keep the mesenteric structure complete, and alleviate inflammatory cell infiltration and splenomegaly, and has outstanding effects. The lactobacillus paracasei can be used for preparing food, health care products, medicines or skin care products with the function of preventing streptococcus infection, and the application prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of screening and application of probiotics, in particular to a strain of Lactobacillus paracasei capable of preventing Streptococcus infection of infants and its application. Background technique [0002] Streptococcus is another common type of bacteria belonging to pyogenes, widely found in nature and in human and animal feces and in the nasopharynx of healthy people. Transient and persistent colonization in the digestive tract or reproductive tract is an opportunistic pathogen. When the immune function of the human body is low, it can cause infection, or cause allergic nephritis, rheumatism, myocarditis and other diseases. According to research statistics, about 10% to 30% of pregnant women are accompanied by GBS infection, and about 50% of pregnant women colonized with GBS will transmit the bacteria to their newborns. In addition, because the infant's immune system is not sound, it is easy to infect the ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/744A61K8/99A61P31/04A61P3/06A61Q19/00A61Q17/00C12R1/225
CPCA23L33/135A61K35/744A61K8/99A61P31/04A61P3/06A61Q19/00A61Q17/005A23V2002/00A23V2400/165A23V2200/30A23V2200/3262A23V2200/324Y02A50/30
Inventor 段治倪海平王倩刘金崔洪昌郭超群张景燕吴松洁步欣萍
Owner 山东百沃生物科技有限公司
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