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Preparation method and application of layered WS2/Au tumor cell membrane composite material

A composite material and cell membrane technology, which is applied in the preparation of layered WS2/Au@tumor cell membrane composite materials and the application field of anti-tumor drug carriers, can solve the limitations of inability to pass through specific biological barriers, easy aggregation of nanoparticles, and clinical application To achieve good tumor treatment effect, good reproducibility, and tumor elimination effect

Active Publication Date: 2022-07-15
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, with more in-depth exploration of nanomedicine, researchers have found that nanoparticles are easy to aggregate and suffer from phagocytosis in the body, and cannot pass through some biological barriers with high specificity, which greatly limits their clinical application.
In addition, exogenous nanoparticles activate the immune recognition system in vivo, leading to accelerated immune clearance upon repeated administration

Method used

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  • Preparation method and application of layered WS2/Au tumor cell membrane composite material
  • Preparation method and application of layered WS2/Au tumor cell membrane composite material
  • Preparation method and application of layered WS2/Au tumor cell membrane composite material

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Experimental program
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Effect test

Embodiment 1

[0033] (1) The commercially available nano-sized layered WS 2 The powder was dispersed in pure water, using an ultrasonic cell disruptor, under the condition of ice-water bath, ultrasonic power 300W for 0.5h to make it dispersed evenly, and it became a small-sized layered WS. 2 (sheet diameter less than 500nm); obtain WS 2 The concentration of the dispersion is 1 mg / ml;

[0034] (2) Take 2mg chloroauric acid and quickly add 10ml WS 2Stir and mix in the dispersion (1mg / ml); after magnetic stirring for 30 minutes, add 1ml (1mg / ml) of sodium borohydride solution dropwise under magnetic stirring, and continue stirring for 8h at an acceleration of 3mg / h; The supernatant was discarded by centrifugation, the unreacted impurities were removed, and the resulting precipitate was ultrasonically dispersed with pure water, which was WS. 2 / Au dispersion at a concentration of 1 mg / ml.

[0035] (3) After the MCF-7 tumor cells adhered to the wall, 1 ml of trypsin was added to digest, and ...

Embodiment 2

[0040] (1) The commercially available nano-sized layered WS 2 The powder was dispersed in pure water, using an ultrasonic cell disruptor, under the condition of ice water bath, ultrasonic power 250W for 0.7h to make it dispersed evenly, and it became a small-sized layered WS. 2 (sheet diameter less than 500nm); obtain WS 2 The concentration of the dispersion is 0.1 mg / ml;

[0041] (2) Take 2mg chloroauric acid and quickly add 8ml WS 2 Stir and mix in the dispersion (1mg / ml); after magnetic stirring for 30 minutes, add 0.8ml (0.5mg / ml) of sodium borohydride solution dropwise under magnetic stirring, and continue stirring for 8h at an acceleration of 4mg / h; the reaction is completed The solution was centrifuged to discard the supernatant, and the unreacted impurities were removed, and the resulting precipitate was ultrasonically dispersed with pure water, which was WS. 2 / Au dispersion at a concentration of 0.1 mg / ml.

[0042] (3) After Hela tumor cells adhered to the wall, ...

Embodiment 3

[0047] (1) The commercially available nano-sized layered WS 2 The powder was dispersed in pure water, using an ultrasonic cell disruptor, under the condition of ice water bath, ultrasonic power 200W for 0.9h to make it evenly dispersed, and it became a small-sized layered WS 2 (sheet diameter less than 500nm); obtain WS 2 The concentration of the dispersion is 1.5 mg / ml;

[0048] (2) Take 2mg chloroauric acid and quickly add 3ml WS 2 Stir and mix in the dispersion (1.5mg / ml); after magnetic stirring for 30 minutes, add 1ml (1.6mg / ml) of sodium borohydride solution dropwise under magnetic stirring, and continue stirring for 8h at an acceleration of 4mg / h; the reaction is completed The solution was centrifuged to discard the supernatant, and the unreacted impurities were removed, and the resulting precipitate was ultrasonically dispersed with pure water, which was WS. 2 / Au dispersion, the concentration is 0.4mg / ml.

[0049] (3) After A549 tumor cells adhered to the wall, 1 ...

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Abstract

The invention belongs to the technical field of material synthesis and biological medicine, and particularly relates to a preparation method and application of a layered WS2 / Au tumor cell membrane composite material. The specific method comprises the following steps: extracting cell membranes from homologous tumor cells by adopting differential centrifugation in combination with cell ultrasonication, mixing and incubating the cell membranes with nanoscale layered WS2 / Au, and obtaining the tumor cell membrane modified layered tungsten disulfide / gold nanoparticle composite material through electrostatic adsorption; the layered tungsten disulfide / gold nanoparticle-cell membrane composite material constructed by the invention has high drug loading capacity and photo-thermal performance, has excellent immune escape ability and homologous tumor cell targeted delivery performance, can be prevented from being recognized and cleared by reticuloendothelial cells, and improves the treatment effect on homologous tumors.

Description

technical field [0001] The invention belongs to the technical field of material synthesis and biomedicine, and in particular relates to a layered WS 2 Preparation method of / Au@tumor cell membrane composite material and its application in antitumor drug carrier. Background technique [0002] Cancer has become one of the greatest threats to human health in modern times. Synthetic nanoparticles have become a research hotspot nowadays due to their high drug loading rate, controllable particle size, and sustainable drug release. In addition to improving the efficacy of drugs, cancer treatments have also improved. From the traditional stage of surgery, chemotherapy, and radiotherapy, it has entered the era of photothermal therapy. Among them, the materials used in it must have excellent photothermal conversion efficiency, high biocompatibility and low toxicity. [0003] Tungsten disulfide is a layered two-dimensional nanomaterial with excellent properties. It has a large spec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/46A61K41/00A61K31/704A61K47/69A61P35/00
CPCA61K31/704A61K41/0052A61K9/5068A61K47/6923A61P35/00
Inventor 招瑞欣谢萌
Owner JIANGSU UNIV
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