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Monoclonal antibody targeting human Claudin18.2 protein and application thereof

A monoclonal antibody, targeting technology, applied in the field of biological cells, can solve problems such as frequent ectopic activation

Active Publication Date: 2022-07-15
SHENZHEN FIRST CONDOR BIOSCIENCE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The expression distributions of Claudin 18.1 and Claudin 18.2 are different, Claudin18.1 is selectively expressed in normal lung cells, and Claudin 18.2 is highly restricted in normal cells, but in a variety of tumors (such as gastric cancer, lung cancer and pancreatic cancer frequent ectopic activation and overexpression in

Method used

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  • Monoclonal antibody targeting human Claudin18.2 protein and application thereof
  • Monoclonal antibody targeting human Claudin18.2 protein and application thereof
  • Monoclonal antibody targeting human Claudin18.2 protein and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0120] The preparation method and functional verification of the antibody of the present embodiment include the following steps:

[0121] S100: Mouse immunization and sequence acquisition of mouse monoclonal antibody

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PUM

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Abstract

The invention relates to the technical field of biological antibodies, in particular to a monoclonal antibody targeting human Claudin18.2 protein and application of the monoclonal antibody. The monoclonal antibody comprises a heavy chain variable region HV and a light chain variable region LV, and the amino acid sequence and the nucleic acid sequence of the monoclonal antibody can be used in a single-chain manner or in a double-chain combined manner. The monoclonal antibody can specifically recognize the human Claudin18.2 antigen, does not recognize the human Claudin18.1 antigen, has good specificity in normal human tissues, is only combined with Claudin18.2 positive tissues, does not recognize other tissues and organs, and has no miss-target risk. The monoclonal antibody has a specific antibody mediated complement dependent (CDC) killing effect on Claudin18.2 positive target cells, and the CAR-T prepared from the monoclonal antibody has a specific killing function on the Claudin18.2 positive target cells.

Description

[0001] This application is a divisional application with an application number of 202111031017X, an application date of September 3, 2021, and the application name is a monoclonal antibody targeting human Claudin18.2 protein and its application. technical field [0002] The invention relates to the technical field of biological cells, in particular to a monoclonal antibody targeting human Claudin18.2 protein, which can be used as a biological material for the construction of other DNA vectors, recombinant protein expression, cell line construction and chimeric antigen receptors In immune cells; or after coupling with other substances, it can be used as a biological agent, or it can be used directly as a biological agent. Background technique [0003] Claudins, a family of proteins that maintain tight junctions that control molecular exchange between cells, are widely distributed in stomach, pancreas, and lung tissues and can be used for disease diagnosis and treatment. The C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/28C07K16/30C12N15/13G01N33/577G01N33/574G01N33/68C07K19/00C12N15/867C12N5/10
CPCC07K16/28C07K16/3046C07K16/3023C07K16/303C07K16/30G01N33/577G01N33/57446G01N33/57438G01N33/57423G01N33/57407G01N33/57492G01N33/68C07K14/7051C12N15/86C12N5/0636C07K2317/56C07K2317/565C07K2317/734C07K2317/92C07K2317/33C07K2317/622G01N2333/705C07K2319/02C07K2319/03C07K2319/33C12N2740/15043C12N2800/107C12N2510/00Y02A50/30
Inventor 谢海涛都晓龙马丽雅
Owner SHENZHEN FIRST CONDOR BIOSCIENCE CO LTD
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