Induction method of actinidia arguta cluster buds
A technology of kiwi fruit and induction medium, which is applied in the field of tissue culture and rapid propagation of kiwi fruit, can solve the problems of low differentiation rate and multiplication coefficient of clustered buds, and achieve the effect of solving the low differentiation rate and multiplication coefficient of clustered buds
Active Publication Date: 2022-07-22
宁德市农业科学研究所
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Problems solved by technology
[0005]
The purpose of the present invention is to provide a method for inducing clustered buds of Actinidia jujube to solve the above-mentioned problems in the prior art. Seedlings, providing technical support for the industrial production of Actinidia jujube tissue culture seedlings
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[0020] Various exemplary embodiments of the present invention will now be described in detail, which detailed description should not be construed as a limitation of the invention, but rather as a more detailed description of certain aspects, features, and embodiments of the invention.
[0021] It should be understood that the terms described in the present invention are only used to describe particular embodiments, and are not used to limit the present invention. Additionally, for numerical ranges in the present disclosure, it should be understood that each intervening value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in that stated range is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
[0022] Unless otherwise ...
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The invention discloses a method for inducing cluster buds of actinidia arguta, and belongs to the technical field of tissue culture and rapid propagation of actinidia arguta. The method comprises the following steps: inoculating a bud-free stem with the seedling age of 30-60 days into an induction culture medium to induce growth of cluster buds, and then inoculating the cluster buds into a rooting culture medium to induce rooting, the induction culture medium comprises 2mg. L <-1 > of MS + ZT; the rooting culture medium comprises 0.4 mg.L <-1 > of MS + IBA. According to the method, the actinidia arguta bud-free stem section is used as an explant, and the influence of different illumination intensities, culture temperatures, seedling ages and plant growth regulators on bud-free stem section callus induction and plant regeneration is researched, so that a rapid, stable and effective actinidia arguta rapid propagation technical system is established and can be effectively applied to production; the method solves the problems of low differentiation rate and multiplication coefficient of cluster buds, can provide a large number of high-quality seedlings in a short time, and provides technical support for factory production of actinidia arguta tissue culture seedlings.
Description
technical field [0001] The invention relates to the technical field of tissue culture and rapid propagation of kiwifruit, in particular to a method for inducing clustered buds of kiwifruit jujube. Background technique [0002] Actinidia arguta (Sieb. & Zucc) Planch.ex Miq., also known as kiwi pear, soft jujube, rattan pear, rattan melon, belongs to Actinidiaceae (Actinidiaceae), Actinidia (Actinidia) large deciduous vines, commonly used The English names include Hardy Kiwifruit, Grape Kiwi and Baby Kiwifruit. As an ancient wild tree species resource, it was first recorded in "Kaibao Materia Medica", and the whole plant is also recorded in "Compendium of Materia Medica", which has a certain medical and health value. Jujube kiwifruit has smooth, hairless and edible peel, delicate and sweet flesh, rich in nutrients, rich in vitamin C and unique flavor. The adult plant has strong cold-resistant characteristics and can safely overwinter at -38°C. It can be directly cultivated o...
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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002Y02P60/14
Inventor 郭慧慧林丛发蒋元斌徐绍翔林泽宇
Owner 宁德市农业科学研究所