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Sheep semen diluent and preparation method thereof

A diluent and semen technology, which is applied in the field of sheep breeding, can solve the problems of difficulty in popularization and application, storage time of only 1-2 days, unstable low-temperature storage effect, etc., and achieve scientific and reasonable results

Pending Publication Date: 2022-07-26
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are commercial sheep semen dilutions on the market, this does not meet the needs of production practice
At present, most farms adopt the natural mating method, and large-scale farms may adopt the method of storing semen at room temperature, because the low-temperature storage effect of the existing diluent formula is unstable, and the storage time may only be 1-2 days, which is difficult to store in semen. Promotion and application in production

Method used

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  • Sheep semen diluent and preparation method thereof
  • Sheep semen diluent and preparation method thereof
  • Sheep semen diluent and preparation method thereof

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiments.

[0030] Materials and Methods;

[0031] 1. Test reagents and consumables;

[0032] D-Fructose, Glucose, Sodium Citrate, Skimmed Milk Powder, Soy Lecithin, Citric Acid, Tris (Tris(Tris), Sodium Penicillin, Streptomycin Sulfate, Fresh Egg, Coomassie Brilliant Blue G-250, 4 % paraformaldehyde, 85% phosphoric acid, 95% alcohol; Mailang sperm automatic analysis system (CASA) was purchased from Nanning Songjingtianlun Biotechnology Co., Ltd.

[0033] 2. The specific operation process;

[0034] In order to make the objectives, technical solutions and beneficial effects of the present invention clearer, the present invention will be further described in detail below with reference to specific examples. The specific examples described herein are only used to explain the present invention, and are not intended to limit the present invention.

[0035] In view of the problems existi...

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Abstract

The invention belongs to the technical field of sheep breeding, and relates to a sheep semen diluent which comprises the following components in parts by mass: 1-2 parts of citric acid, 1.5-2.5 parts of fructose, 2-4 parts of Tris, 5-18 parts of yolk, 0.1-0.4 part of penicillin sodium, 0.1-0.4 part of streptomycin sulfate and 100 parts of ultrapure water. According to the invention, the preservation time of the sheep semen is effectively prolonged, slow and stable reduction of sperm movement performance is maintained, and damage to sperm structures such as plasma membranes and acrosomes is slowed down. The invention provides the diluent suitable for sheep semen preservation, and the semen preservation quality is remarkably improved.

Description

technical field [0001] The invention relates to a sheep semen diluent and a preparation method thereof, and belongs to the technical field of sheep breeding. Background technique [0002] At present, the preservation of sheep semen is mainly based on cryopreservation, and the effective fertilization time in production practice is 3 days. When sheep semen leaves the body for in vitro storage, sperm metabolism does not stop. Therefore, the diluent should contain carbohydrates to satisfy sperm metabolism, buffer substances to maintain normal osmotic pressure, and antifreeze substances to prevent cold shock caused by low temperature. Although there are commercial ovine semen diluents on the market, this does not meet the needs of production practice. At present, most of the farms use natural mating methods. Large-scale farms may use the method of storing semen at room temperature, because the low-temperature preservation effect of the existing dilution formula is unstable, and...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0221A01N1/0226
Inventor 李拥军张柳明王彦虎王强康言孙晓梅
Owner YANGZHOU UNIV
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