Multi-channel parallel two-stage reaction centrifugal micro-fluidic chip
A microfluidic chip and secondary reaction technology, applied in chemical/physical/physical chemical mobile reactors, laboratory containers, chemical/physical/physical chemical processes, etc., can solve endogenous interference, stabilize Low accuracy, limited chip detection performance and application range, etc.
Pending Publication Date: 2022-07-29
广东长光中科生物科技有限公司
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Problems solved by technology
[0003] The parallel reaction detection channel of the existing centrifugal microfluidic chip only contains a first-level reaction structure, and only a single reagent can be used, which greatly limits the detection performance and application range of the chip
Taking biochemical detection as an example, there are problems such as endogenous interference and low stability and accuracy in the single-reagent reaction process, which have been gradually replaced by double-reagents on traditional large-scale equipment.
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[0030] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.
[0031] In order to make those skilled in the art better understand the solution of the present application, the present application will be further described in detail below with reference to the accompanying drawings and specific embodiments.
[0032] Please refer to figure 1 , figure 1 This is a schematic structural diagram of the multi-channel parallel two-stage reaction centrifugal microfluidic chip provided in the embodiment of th...
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The invention discloses a multichannel parallel two-stage reaction centrifugal micro-fluidic chip which comprises a first connecting channel, a second connecting channel and a third connecting channel, the front end of the first connecting channel is provided with an inlet, and the tail end is provided with a second siphon valve and a waste liquid cavity; the second connecting channel is communicated with a second-stage reaction cavity; the first end of the third connecting channel is communicated with the first connecting channel, the second end of the third connecting channel is communicated with the first end of the first-stage reaction cavity, a first air pressure balance channel communicated with the second end of the second connecting channel is further arranged, and a buffer cavity is formed between the first end and the second end of the third connecting channel; and a first siphon valve communicated with the first end of the second connecting channel is arranged at the second end of the first-stage reaction cavity. According to the multichannel parallel two-stage reaction centrifugal micro-fluidic chip, the requirement of double-reagent two-step reaction can be met; the shunting quantification and residence of the multi-channel parallel first-stage reaction cavity and the controllable conveying from the first-stage reaction cavity to the second-stage reaction cavity are realized.
Description
technical field [0001] The present application relates to the technical field of centrifugal microfluidic chips, in particular to a multi-channel parallel two-stage reaction centrifugal microfluidic chip. Background technique [0002] The centrifugal microfluidic chip is a microfluidic system that realizes the detection and analysis of liquid flow. It miniaturizes and integrates the valves, flow pipes, mixers, reactors, separation devices and other components involved in the chemical analysis process on a CD-shaped chip. And use centrifugal force as the driving force of liquid flow. [0003] The parallel reaction detection channel of the existing centrifugal microfluidic chip only includes a primary reaction structure and can only use a single reagent, which greatly limits the detection performance and application range of the chip. Taking biochemical detection as an example, there are problems such as endogenous interference and low stability and accuracy in the single-rea...
Claims
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IPC IPC(8): B01J19/00B01J19/28B01L3/00
CPCB01J19/0093B01L3/5027B01J19/28B01J19/0053B01J19/0046B01L2200/027B01L2300/0861
Inventor 徐阳吴一辉
Owner 广东长光中科生物科技有限公司