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Regulating-control method for preparing different polymerization degree compound sugar by enzymatical method

A technology for enzymatic preparation and oligosaccharide, applied in the direction of oligosaccharide, can solve the problems of affecting large-scale production, complicated operation, serious pollution, etc., and achieve the effect of improving use efficiency, reducing pollution and improving separation efficiency

Inactive Publication Date: 2004-09-01
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods can realize the effective separation of active chitosan oligosaccharides, they affect large-scale production due to complicated operations, serious pollution and high cost.

Method used

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  • Regulating-control method for preparing different polymerization degree compound sugar by enzymatical method
  • Regulating-control method for preparing different polymerization degree compound sugar by enzymatical method

Examples

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Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: in enzyme degradation reactor 1, 0.5% chitosan is dissolved in pH5.6, concentration is in the sodium acetate buffer solution of 0.05M, filter and remove residue, add 1% chitosan enzyme to be degradation enzyme, react The temperature is 35° C., the reaction time is 4 hours, the chitosan is degraded, and the ultrafiltration can be performed after the viscosity of the solution drops to 40-60%. Hollow fiber membrane ultrafilter Roll-type flat membrane nanofilter is assembled into a membrane module 2 and connected to enzyme degradation reactor 1 through infusion pump 3 to separate oligosaccharides with different degrees of polymerization. The surface area of ​​the hollow fiber ultrafiltration membrane is 2m 2 , the cut molecular weight is 2,000-10,000, the part smaller than the molecular weight cut off passes through the membrane to obtain ultrafiltrate, and the molecules larger than the molecular weight cut off return to the enzyme degradation reactor 1 throug...

Embodiment 2

[0015] Embodiment 2: 2% pectin is dissolved in the aqueous solution of 20L, add the pectinase of 3.0%, reaction temperature 50 ℃, reaction time 1 hour, pH3.0, flat membrane ultrafilter ultrafilter, adopt intermittent feed , the rest of the operations are the same as in Example 1 to obtain pectin oligosaccharides with a degree of polymerization of 3-20.

Embodiment 3

[0016] Example 3: 0.5% lipase was added to 3% carrageenan aqueous solution, the reaction temperature was 80° C., pH 10, and the rest of the operations were the same as in Example 1 to obtain carrageenan oligosaccharides with a degree of polymerization of 3-18.

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Abstract

A control method for preparing oligoses with different polymerization degrees by enzyme method features that the enzyme method for degradating polyose and membrane separation are combined, and includes such steps as choosing different membrane media and pore diameters, connecting ultrafilter with enzyme degradation reactor, separating the oligoses with different polymerization degrees, ultrafilter, and nanometer filter to remove active monoose, diose and water.

Description

technical field [0001] The invention belongs to a method for regulating the degree of polymerization of oligosaccharides, in particular to a method for regulating the degree of polymerization of oligosaccharides prepared by enzymatic method. Background technique [0002] Oligosaccharides generally refer to functional molecules composed of 2-10 monosaccharides and have certain physiological activities. The main functions are: can promote the proliferation of bifidobacteria, inhibit the harmful growth of intestinal tract, such as oligosaccharides, etc.; have the ability to inhibit tumor growth, enhance the body's immunity, and reduce blood cholesterol, such as oligomeric grapes; It can hinder the growth and reproduction of pathogenic bacteria and enhance the defense ability of plants against pests and diseases, such as galactooligosaccharides. Therefore, it is widely used in medicine, health food and agriculture. Oligosaccharides are usually obtained by degrading polysacchar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H3/06
Inventor 杜昱光白雪芳曲天明李曙光
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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