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Method for culturing rape line suitable for Al-contained acidic red soil red soil by biologic engineering

A bioengineering, acid red technology, applied in the field of cultivation of crop rapeseed strains, can solve problems such as poor aluminum resistance

Inactive Publication Date: 2004-12-15
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rapeseed cultivars popularized in my country currently have poor tolerance to aluminum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Take rape cotyledons or hypocotyls (Zhongyou 119) → in vitro culture on MS+NAA 1mg / L+6-Ba 1mg / L+2, 4D 1mg / L medium to induce callus →use 0.2% EMS (Ethyl methylsulfonate) was treated for 12----36 hours → transferred to a medium without mutagen for culture and proliferation → with a weight concentration of 100ppm AlCl 3 Select culture medium → transfer the surviving callus to AlCl-free medium 3 Culture and proliferation in the culture medium → with a weight concentration of 200ppmAlCl 3 Select culture medium → transfer the surviving callus to AlCl-free medium 3 Proliferation in the culture medium → with a weight concentration of 300ppm AlCl 3 Select culture medium → transfer the surviving callus to AlCl-free medium 3 Cultivate and proliferate in the culture medium → regenerate plants on MS+NAA0.2mg / L+6-BA5mg / L medium → transplant to red soil for growth → undergo 3----5 generations of bagging selfing → obtain seeds → Al-tolerant rapeseed lines adapted to acidic red ...

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Abstract

A bio-engineering method for culturing the novel rape line suitable for Al-containing acidic red (or yellow) soil features use of distant hybridization or mutant screening. The said distant hybridization includes introducing the Al-resistant gene of radish to cole, continuous backcrosses and radiating or tissue culture to integrate the Al-resistant gene of radish into the rape genome. The said mutant screening includes using physicochemical mutagen to treat the rape tissue cells or seed, culturing in vitro, choosing Al-resistant mutant cells, regenerative culture, transplanting to said red (yellow) soil and selfing by several generations. The resultant rapeline features high resistance to Al toxin and high yield.

Description

technical field [0001] The invention relates to a method for cultivating rape strains of crops, in particular to a method for cultivating rape strains adapted to growth in aluminum-containing acidic red soil by using bioengineering. technical background [0002] Aluminum is the most abundant metal element in the earth's crust, usually in the form of insoluble alumina or silicate, which is not toxic to crops, but in acidic conditions, soluble Al 3+ Toxic to most crops. Therefore, the acid resistance of crops is consistent with the tolerance to aluminum toxicity, and aluminum toxicity is the most important problem of crop growth on acidic red soil (Kochian et al., 1995). Red loam is an important agricultural soil in southern my country, accounting for 21% of the total land area of ​​the country. At present, the rapeseed varieties popularized in my country generally have poor aluminum tolerance. The yield of rape planted on red soil is only about 798K...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00A01H1/02A01H4/00
Inventor 黄邦全黄邦莲
Owner HUBEI UNIV