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Method for raising activity of streptoverticillium to produce trans glutaminase

A technology of transglutaminase and Streptomyces verticillium, applied in the field of enzyme engineering, can solve problems such as unstable enzyme production

Inactive Publication Date: 2005-07-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the research on the production of transglutaminase by Streptomyces verticillium SK4.001 (preserved by the Food Science Research Laboratory of Jiangnan University), it was found that there was a problem of unstable enzyme production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Streptoverticillium (Streptoverticillium) SK4.001 is the production strain, and the composition of the liquid medium is the above composition, adding ZnSO 4 In liquid medium, Zn 2+ The addition amount is 3ug / mL, and the culture is based on sterilizing at 121°C for 20 minutes before use. Take a ring of fresh slant bacteria, put it into a 250mL Erlenmeyer flask containing 25mL liquid medium, and cultivate it at 30°C and 210rpm for 24h. Inoculate 4% of the inoculum into 25mL liquid medium, and culture continuously for 60 hours at 30°C and 210rpm to obtain a maximum dry cell weight of 16.99mg / mL and a transglutaminase activity of 2.289U / mL.

Embodiment 2

[0050] Add ZnSO 4 In liquid medium, Zn 2+ The addition amount was 8ug / mL, and the rest of the operations were the same as in Example 1. The maximum dry cell weight was 17.11mg / mL, and the activity of transglutaminase was 4.723U / mL.

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PUM

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Abstract

The method of raising activity of streptoverticillium to produce transglutaminase belongs to the field of enzyme engineering technology. Streptoverticillium, such as streptoverticillium SK4.001, is used as production strain and Zn ion in different amount is added to culture medium, the thallus amount, pH value, redisual glycerin and transglutaminase activity are detected. It is found that Zn ion can meet the need of promoting the thallus growth in streptoverticillium SK4.001 transglutaminase, and promote the enzyme production of transglutaminase. The amount of Zn ion in the culture medium in 3 microgram / ml has relatively high thallus amount, and further increase of the amount of Zn ion to 8 microgram / ml results in no obvious change in thallus amount but doubled transglutaminase activity, 4.723 U / ml.

Description

technical field [0001] The invention discloses a method for improving the activity of transglutaminase produced by Streptomyces verticillium, belonging to the technical field of enzyme engineering. Background technique [0002] Transglutaminase, also known as transglutaminase (Transglutaminase, EC 2.3.2.13, referred to as TGase), is a transferase that catalyzes the acyl transfer reaction. It can catalyze the intramolecular and intermolecular cross-linking reactions of proteins such as casein, lactoglobulin, myosin, gluten, etc., thereby changing the functional properties of proteins, so it has a good application prospect in the food industry, but animals and The high cost of plant tissue-derived transglutaminase hinders its further promotion in the food industry. [0003] In 1989, Japan Ajinomoto Co., Ltd. and Amano Pharmaceutical Co., Ltd. cooperated to screen out Streptoverticillium with high transglutaminase activity from more than 5,000 strains of actinomycetes, and cre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/80
Inventor 江波王璋曾新
Owner JIANGNAN UNIV